Histological and Histochemical Characterization of the Mucosa of the Digestive Tract in Flower Fish (<i>Pseudophoxınus antalyae)</i>

Çınar, Kenan; Şenol, Nurgül

doi:10.1111/j.1439-0264.2005.00629.x

Cited by 35 publications

(28 citation statements)

References 20 publications

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“…This pattern of ample folds of the mucosa is common in carnivores, acting to expand the surface absorption area, since carnivores have a relatively short intestine compared to animals with other feeding habits [30] . Sections of the lining epithelium of both regions, when submitted to the PAS and AB histochemical protocols, reacted positively, showing cells with pink (PAS) and blue (AB) coloring, indicating the presence of neutral and acid GCs, respectively, as also observed by Cinar et al [32] in Pseudophoxinus antalyae, by Carrassón et al [21] in D. dentex and by Leknes [26] in H. anisitsi, but unlike that observed in P. fulvidraco [25] and Chanos chanos [29] . This allows the hindgut to lubricate the tube and to trap particles to be eliminated, permitting the food bolus to reach this region in dehydrated form [33] .…”

Section: Discussionsupporting

confidence: 48%

Immunohistochemical study of the digestive tract ofOligosarcus hepsetus

Vieira-Lopes

Pinheiro²,

Sales³

et al. 2013

WJG

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AIM:To describe the histology of the digestive tract and to investigate the occurrence of endocrine cells in Oligosarcus hepsetus (O. hepsetus ). METHODS:The digestive tract (DT) of O. hepsetus was divided into esophagus, two stomach regions (glandular and non-glandular) and two intestinal regions (anterior and posterior). These specimens were processed by routine histological techniques and stained with hematoxylin-eosin, Gomori's trichrome, periodic acid Schiff (PAS) and Alcian blue (AB). An immunohistochemical method using avidin-biotin-peroxidase was employed. RESULTS:The esophagus is lined with a non-keratinized stratified squamous epithelium that is reactive to PAS and AB. The stomach has a mucosa lined with a simple columnar epithelium with mucus-secreting cells that are reactive only to PAS. The intestine has a simple columnar epithelium with a brush border and goblet cells that are reactive to PAS and AB. Somatostatin, serotonin and cholecystokinin immunoreactive cells were identified throughout the DT. CONCLUSION:This study revealed adaptations for the species' diet and showed that the distribution and relative frequency of immunoreactive cells are similar to those of other fish.

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Section: Discussionsupporting

confidence: 48%

Immunohistochemical study of the digestive tract ofOligosarcus hepsetus

Vieira-Lopes

Pinheiro²,

Sales³

et al. 2013

WJG

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“…The results of the present work revealed some differences in the structure of the oesophagus of both species related to type of food and feeding habits of both species. The gastrointestinal tracts of fish show remarkable differences in function and structure, these differences were related to type of food, feeding habits, body weight, shape, and sex [16][17][18].…”

Section: Discussionmentioning

confidence: 99%

Comparative Histomorphological Studies on Oesophagus of Catfish and Grass Carp

Hafez

Mokhtar

Abou-Elhamd

et al. 2013

Journal of Histology

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The present work was carried out on 40 specimens of oesophaguses of both sexes of catfish (carnivorous fish) and grass carp (herbivorous fish) in order to observe the morphological and histological differences between the two species. Oesophagus of catfish was divided into 2 parts: anterior and posterior ones. The anterior part of the oesophagus of catfish was characterized by the presence of numerous mucosal folds. It was lined by stratified epithelium with goblet cells. In addition to club cells were observed in between the stratified epithelium. Scanning electron examination of the oesophageal epithelium of catfish demonstrated the presence of microvilli and fingerprint-like microridges in the superficial cell layer. The posterior part of the oesophagus of catfish was characterized by simple columnar mucus-secreting epithelium. The oesophagus of grass carp had shown the same structure along its entire length. It consisted of less folded mucosa than that observed in the oesophagus of catfish. The epithelium was characterized by the presence of taste buds. In conclusion, the present work revealed some differences in the structure of catfish oesophagus and grass carp oesophagus. These differences are related to type of food and feeding habits of each species.

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“…The presence of MC is a common feature in the intestinal epithelium of teleosts (Grau et al, 1992;Çinar & Şenol, 2006;Hernández, Pérez Gianeselli, & Domitrovic, 2009). In the I1 of C. paleatus, the MC were scarce and had a low content of highly sulfated mucins and a high content of weak acid mucins.…”

Section: Discussionmentioning

confidence: 99%

Histochemical differences along the intestine of Corydoras paleatus (Siluriformes: Callichthyidae)

Plaul

Barbeito

Díaz

2016

RBT

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Abstract:The Neotropical catfish Corydoras paleatus is a facultative air-breather and the caudal half of the intestine is involved in gas exchange. In South America, air-breathing fishes are found in tropical or sub-tropical freshwaters where the probability of hypoxia is high. The aim of this study was to characterize by traditional histochemical and lectinhistochemical methods the pattern of carbohydrate in the intestinal mucosa. Intestine samples were taken from 25 healthy adult specimens collected in Buenos Aires (Argentina). Samples were fixed by immersion in 10 % buffered formalin and routinely processed and embedded in paraffin wax. Subsequently, these sections were incubated in the biotinylated lectins battery. Labeled Streptavidin-Biotin (LSAB) system was used for detection, diaminobenzidine as chromogen and haematoxylin as a contrast. To locate and distinguish glycoconjugates (GCs) of the globet cells, we used the following histochemical methods: PAS; PAS*S; KOH/ PA*S; PA/Bh/KOH/PAS; KOH/PA*/Bh/PAS; Alcian Blue and Toluidine Blue at different pHs. Microscopically, the general structure of vertebrate intestine was observed and showed all the cell types characteristic of the intestinal epithelium. The cranial sector of catfish intestine is a site of digestion and absorption and its structure is similar to other fish groups. In contrast, enterocytes of the caudal portion are low cuboidal cells; and between these, globet cells and capillaries are observed, these latter may reach the mucosal lumen. Underlying the epithelium, observed a well-developed lamina propria-submucosa made of connective tissue; this layer was highly vascularized and did not exhibit glands. According to histochemistry, the diverse GCs elaborated and secreted in the intestine are associated with specific functions in relation to their physiological significance, with special reference to their role in lubrication, buffering effect and prevention of proteolytic damage to the epithelium together with other biological processes, such as osmoregulation and ion exchange. The lectinhistochemical analysis of the intestinal mucosa reveals the presence of terminal residues of glucose, mannose and galactose. In conclusion, this study has shown that GCs synthesized in the intestine of C. paleatus exhibit a high level of histochemical complexity and that the lectin binding pattern of the intestinal mucosa is characteristic of each species and the variations are related with the multiple functions performed by the mucus in the digestive tract. The information generated here may be a relevant biological tool for comparing and analyzing the possible glycosidic changes in the intestinal mucus under different conditions, such as changes in diet or different pathological stages. Rev. Biol. Trop. 64 (1): 327-340. Epub 2016 March 01.

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Histological and Histochemical Characterization of the Mucosa of the Digestive Tract in Flower Fish (Pseudophoxınus antalyae)

Cited by 35 publications

References 20 publications

Immunohistochemical study of the digestive tract ofOligosarcus hepsetus

Immunohistochemical study of the digestive tract ofOligosarcus hepsetus

Comparative Histomorphological Studies on Oesophagus of Catfish and Grass Carp

Histochemical differences along the intestine of Corydoras paleatus (Siluriformes: Callichthyidae)

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