Histological and Histochemical Studies on the Harderian Gland of the Osprey (Pandion haliaetus)

Rev. Bras. Cienc. Avic.

Kowalczyk

et al. 2016

Harderian gland, histology, histochemistry, common pheasant, hybrid of Italian amber and common pheasant. ABStRACtThe aim of the present study was to demonstrate the histological and histochemical structure of the Harderian gland in wild and hybrid of wild and domestic birds. The samples were stained with haematoxylin-eosin, methyl green-pyronin Y, periodic acid-Schiff, alcian blue pH 2.5, aldehyde fuchsin and Hale's dialyzed iron staining's. In both species, the glands had multilobar tubuloacinar structure type I. The Harderian gland was located in the orbit near the inter-orbital septum, between the medial rectus muscle, the pyramidal muscle of the third eyelid, and the ventral oblique muscle. In the common pheasant, the gland was wider in the proximal and distal part. The common pheasant had more elongated lobes of the Harderian gland than in the hybrid. In the common pheasant, the glandular cells presented darkly-stained serous secretion and lightly-stained mucous secretion. In the hybrid, the glandular cells presented seromucous secretion. The central lobar space, interacinar space, and apical parts of the acini of the Harderian glands were filled with many lymphocytes and plasma cells, particularly in the common pheasant, where centers of all large lobes were abundantly filled with plasma cells. The plasma cells dominated in common pheasant's Harderian gland, while in the hybrid, lymphocytes and plasma cells were present at similar quantities. The cells positive for periodic acid of Schiff staining were dominant in hybrid. Periodic acid-Schiff, Hale's dialyzed iron and alcian blue pH 2.5 stainings demonstrated acid-carboxylated mucopolysaccharides in the glandular cells cytoplasm of the examined birds.

Section: Structural Differences Of the Harderian Gland Between Commonsupporting

confidence: 71%

Section: Structural Differences Of the Harderian Gland Between Commonmentioning

confidence: 49%

“…These two types of cells were also described by Kozlu et al (2010) in the HG of osprey and by Frahmand & Mohammadpour (2015) in the HG of Canadian ostrich.…”

Section: Structural Differences Of the Harderian Gland Between Commonmentioning

confidence: 90%

Section: Structural Differences Of the Harderian Gland Between Commonmentioning

confidence: 99%

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Structural Differences of the Harderian Gland between Common Pheasants (Phasianus Colchicus Talischensis) and Hybrids of Italian Amber and Common Pheasants

Rev. Bras. Cienc. Avic.

Kowalczyk

et al. 2016

“…In our study, the HG of the Capercaillies was located ventromedially, near the inter-orbital septum of the orbit, between the medial straight muscle, pyramidal third eyelid muscle and ventral oblique muscle. This location was similar to that of other avian species [2,7,30,44]. In the examined birds, the HG was flattened and elongated in shape and light pink in colour.…”

Section: Discussionsupporting

confidence: 52%

Histological, histochemical and ultrastructural studies on Harderian and lacrimal glands of the Capercaillie (Tetrao urogallus majorL.)

Acta Biologica Hungarica

Kowalczyk

et al. 2016

This study describes the macroscopic anatomy and the microscopic and ultrastructural features of the Harderian gland and lacrimal gland of the Capercaillies. It was conducted both on adult male and female Capercaillies. Tissue sections were stained with hematoxylin and eosin, azan trichrome, modified Mallory's trichrome, methyl green-pyronin Y, periodic acid-Schiff, alcian blue pH 2.5, aldehyde fuchsin and Hale's dialysed iron. The morphometric study of the Harderian and lacrimal glands indicated that they are both larger in male than in female Capercaillies. The histological analysis showed that the HG has a multilobar tubulo-alveolar structure with numerous lymphocytes and plasma cells. The LG has a multilobar tubulo-acinar structure without lymphocytes and plasma cells. The periodic acid-Schiff staining and alcian blue pH 2.5 staining demonstrated a mild positive reaction in the epithelial cells of the Harderian gland and weak positive reaction in the lacrimal gland. The HDI staining detected the presence of carboxylated acid mucopolysaccharides in the Harderian and lacrimal glands. Transmission electron microscopy revealed the presence of two types of secretory vesicles in the cytoplasm of both studied glands. It also showed that lipid droplets and glycogen granules were more abundant in the Harderian gland than in the lacrimal gland of this species.

Morphological Studies on the Harderian Gland in the Ostrich (Struthio camelus domesticus) on the Embryonic and Post-natal Period

Barszcz

et al. 2014

Anat. Histol. Embryol.

The present investigation was performed on 50 ostriches from 28th day of incubation until the 7th month of life. The morphological (morphometric, histological, histometric and histochemical) studies were conducted. Tissue sections were stained with haematoxylin-eosin, methyl green-pyronin Y, periodic acid-Schiff, alcian blue pH 2.5, aldehyde fuchsin and Hale's dialyzed iron studies. The Harderian gland becomes macroscopically visible on the 28th day of incubation. It is situated in the ventronasal angle of the orbit near inter-orbital septum, between medial rectus muscle, pyramidal and ventral oblique muscles. The Harderian gland of ostrich is a tubulo-acinar gland. The acini were composed of tall conical cells which formed a small lumen and were surrounded by myoepithelial cells. These cells had a granular basophilic, vacuolated cytoplasm. Each of the lobes has a system of complex branching ducts - tertiary, secondary and primary. In the III of research group (3rd week of life), the presence of few plasma cells was demonstrated, which were located within acini and tertiary and secondary ducts, whereas the biggest concentration of plasma cells was observed in group IV of research tissue (4th month of life). The dark cells were observed first time in main ducts 72 h after hatching of nestlings (group II). The morphometric and histometric studies showed that the most intensive growth of Harderian gland occurred between the third week and the seventh month of birds' life. The histochemical study indicated the presence of neutral and acidic mucins, glycoproteins and carboxylated acid mucopolysaccharides.