Histological and immunohistochemical investigations of ovarian interstitial glands during non‐breeding season in camels (Camelus dromedarius)

Abstract

The aim of this was to investigate the histology and immunohistochemistry of interstitial glands during non-breeding season in camel ovaries. A total of 21 mature, non-pregnant and apparently healthy camels aged between 8 and 12 years were slaughtered. The ovaries were removed within 15 min, cleaned from adipose tissue, weighted and examined grossly. The histological preparation was made, and then, the blocks were cut at 3-5 microns thickness and stained by H&E for histological examinations. Moreover, some sections were stained with Sudan Black for lipid detection. Immunohistochemical analysis of paraffin-embedded ovarian tissues was performed to detect the localization of S-100, vimentin, progesterone receptors (PR) and oestrogen receptors (ER). Immunoreactive signals were detected using UltraVision Detection System. The results revealed that the interstitial glands were located in the cortical region and they were arranged in various arrangements either single, in couple or in groups rich in lipid droplet. All interstitial gland arrangements were enclosed by connective tissue capsules containing fibroblasts and collagenous fibres separated them from the surrounding ovarian structures. Both interstitial glands and their surrounding CT were penetrated by several blood vessels. There was a strong immunoreactive signal for S-100 in the nuclei of interstitial cells, and no signals were detected either in cells of the interstitial glands or their connective tissue with PR. We could conclude that the interstitial gland is distinct in ovary of camel and further studies are needed to elucidate its rule in steroid synthesis.

Spatial Transcriptomics of Aging Rat Ovaries Reveals Unexplored Cell Subpopulations with Reduced Antioxidative Defense

“…Besides, an increase in the proportions of interstitial gland cells with higher expression of androgen synthesis-related genes was detected in aged ovary. In rodent species, the interstitial gland comprises a complex population of androgen-producing cells which proliferate during the growth and differential of the ovary [48][49][50][51]. These cells are thought to arise primarily from theca components and possess functions similar to theca cells [52].…”

The relationship between ovarian hormones and mast cell distribution in the ovaries of dromedary camel (Camelus dromedaries) during the follicular wave

“…In comparison to other seasons, the ovary in the non-breeding season showed less activity, fewer follicles, and more interstitial tissue. In contrast, the camel’s ovary showed more activity and many follicles during the breeding season [ 32 ]. Because of this, we did not observe the MC distribution during the non-breeding season.…”

“…Ultrasonographic examinations were completed for all ovaries using an ultrasound device (Eickemeyer Magic 500 Digital, Germany) integrated with a linear probe (5.0–7.5 MHz). Tissue samples from the ovaries, including luteinized follicles, were classified into the following phases: Growing phase (n = 12 animals, presence of 0.3–0.8 cm follicle[s] Ø), mature phase (n = 9 animals, 0.9–2.2 cm follicle[s] Ø), and regression phase (n = 7, oversized follicle[s] >2.5 cm), as shown in Figures- 1a and b [ 32 ].…”

Paraffin-embedding for large volume bio-tissue

“…Although FFPE had a great potential in embedding large brains, traditional FFPE was usually used to embed small samples in 0.5–1 cm-thickness 15 . Clearing was a key factor, which limited the size of samples in paraffin embedding process.…”

“…It was convenient for long-term imaging. Finally, FFPE allowed semithin successive sections while leaving sections amenable to further characterization such as hematoxylin-eosin staining (H&E), hybridization in situ (ISH) 13 Although FFPE had a great potential in embedding large brains, traditional FFPE was usually used to embed small samples in 0.5-1 cm-thickness 15 . Clearing was a key factor, which limited the size of samples in paraffin embedding process.…”