Histological and Immunohistochemical Study of the Effect of Alendronate on the Submandibular Salivary Gland of Adult Male Albino Rat and the Possible Protective Effect of Propolis

Abstract

Background: Alendronate is an anti-bone-resorptive drug used in the treatment of post-menopausal osteoporosis. Its long administration induced organ damage especially the submandibular salivary glands. Propolis is a natural resinous substance with an antioxidant and anti-inflammatory actions. Aim of Study:This work was performed to study the effect of alendronate on the histological structure of the submandibular salivary gland of adult male albino rat and the possible protective effect of propolis.Material and Methods: 40 adult male albino rats were divided into 4 groups (10 rats each); control group, Group A: Rats received 100mg/kg body weight propolis, Group B: Rats received 2.5mg/kg body weight alendronate and Group C: Rats received propolis 30 minutes before alendronate. Doses were given orally for consecutive 30 days then gland specimens were taken for histological and immunohistochemical studies using light and electron microscopes.Results: H & E and toluidine blue results of Group B revealed disturbed architecture, separated lobules and acini, cytoplasmic vacuolations, dark nuclei, irregular ducts and congested blood vessels. PAS-AB showed significant increase in its mean color intensity as compared with control group while a-SMA glandular myoepithelial cells immunostaining expressed significant decrease in its mean color intensity in comparison to control group. Electron microscopic examination of acinar and duct cells revealed cytoplasmic vacuolations, rarefaction, abnormal shaped mitochondria with lost cristae, electron dense bodies, dilated RER and Golgi apparatus, apparently increased secretory granules with coalescence, compressed shrunken hyperchromatic nuclei, nuclear membrane irregularities, and dilated perinuclear and intercellular spaces. Group C showed moderate improvement of the previous pathological findings.Conclusion: Alendronate induced damaging effects on the histological structure of submandibular salivary gland in Egypt rats and the administration of propolis could moderately improve such damage through its anti-oxidant and antiinflammatory effects.

Effects of aflatoxin B1 on the submandibular salivary gland of albino rats and possible therapeutic potential of Rosmarinus officinalis: a light and electron microscopic study

“…Coppes et al 25 attributed the presence of acinar intracytoplasmic vacuoles to intra-cytoplasmic degenerative changes caused by the presence of proteolytic enzymes in their secretory granules which might infiltrate and cause damage to the cytoplasm, leading to autolysis and cellular death. However, Samah et al 26 suggested that cytoplasmic vacuolations seen in acinar, GCT and excretory duct cells might be due to the ROS formation, resulting in lipid peroxidation and damage of cell membranes as well as membranes of the cell organelles, ending in impairment of the energy-dependent Na+ K+ ion pumps in the cell. These changes lead to accumulation of Na+ inside the cells with entry of water resulting in cellular swelling and vacuolations.…”