Histological and immunohistochemical study of the potential healing effects of glucosamine and platelets rich plasma on experimentally induced oral mucositis in adult male rats

Abstract

Background: Oral mucositis (OM) is mucosal barrier injury. It is commonly caused by 5 Flurouracil (5-FU). Glucosamine (GlcN) has many beneficial therapeutic effects. Platelets rich plasma (PRP) are a promising line for curing ofvarious clinical injuries. Objective: The aim of the study was to evaluate the effect of glucosamine and PRP on experimentally induced oral mucositis in adult male rats. Materials and methods: 40 adult male rats divided evenly into four groups. Group I (Control). Group II (Mucositis): rats injected one time intraperitoneally with 5-FU(150 mg/kg body weight).Group (III) (Mucositis treated with glucosamine): rats injected intraperitoneally with glucosamine (1000mg/kg body weight) following induction of oral mucositis for 3weeks. Group IV (Mucositis treated with PRP) rats injected with PRP(1ml/rat/day) locally into buccal mucosa following induction of oral mucositis for 3weeks. Buccal mucosa samples prepared and examined by using histological and immunohistochemical techniques. Results: Group II showed apparent diminish in the thickness of the epithelium with separation of keratin, hemorrhage and perinuclear vacuolization. There were areas of degeneration, intense subepithelial mononuclear infiltration and cell apoptosis (highly expressed caspase-3). A significant reduction of Periodic acid Schiff (PAS) positive reaction and anti-proliferating cell nuclear antigen (PCNA) positive nuclei (P<0.01). Group III&IV showed improvement of the histological and immunohistochemical changes described before. Conclusion: Glucosamine and PRP provide a promising treatment for oral mucositis accompanying with chemotherapy. PRP remedy is simple and the most efficient mean as it exhibits more rapid epithelial differentiation and wound healing.

Evaluation of the impact of platelet rich plasma and hyaluronic acid on ketamine-induced cystitis in albino rats : A histological and immunohistochemical study.

“…Proliferating cell nuclear antigen (PCNA) is a nonhistone nuclear protein that used as a standard marker for cellular proliferation activity [36] . Our findings were coincided with previous researches [12,23,37] , who stated that urothelium is a very stable type of tissue and has a slow proliferative activity. However, after exposure to an injury, which causes loss of superficial cells, tight junctions are damaged, and the proliferative activity of the basal urothelial cells are increased rapidly Desmin is the predominant intermediate filament protein in the visceral smooth muscle [38] .…”

“…Other researches [11,12,44] attributed the ability of PRP to accelerate the epithelial cell regeneration to the biological effects of many growth factors and different cytokines as platelet derived growth factor, vascular endothelial growth factor and transforming growth factor of platelets. These growth factor leads to cell proliferation of epithelial cells, promote tissue angiogenesis, increase blood flow, and improve oxygenation in the site of injury.…”

“…It has major advantages compared with other methods on clinical purposes as it is a cheap product, easily obtained, and being autologous diminishes the hazards of rejection or immune response. Additionally, PRP has an antimicrobial effect as it contains leukocytes, resulting of diminishing the risk of infection [9,10,12,13] .…”

Regenerative and Antioxidant Properties of Autologous Platelet-Rich Plasma Can Reserve the Aging Process of the Cornea in the Rat Model

“…They explained that due to a high concentration of platelets that contained growth factors attached to the corneal surface and accelerate tissue repair mechanism. Zheng et al [ 16 ] and Mansy et al [ 50 ] mentioned that PRP played a dominant role in cell migration and proliferation.…”

Effect of the Long-Term Gum Arabic Solutions Preservation on Histological Structure of Biological Tissues

“…Subsequently, pieces of 1 cm 3 size were cut from fresh (as a negative control) of each heart, kidney, and brain of adult sheep after the slaughter at an abattoir and fixed immediately after collection in 10% formalin (02144.CHEMANOL. KSA) for two days [6].…”