Histological and immunohistochemical study of the effect of experimentally induced hypothyroidism on the thyroid gland and bone of male albino rats

Elkalawy, Seham A. M.; Abo-Elnour, Rahma K.; Deeb, Dalia F. El; Yousry, Marwa Mohamed

doi:10.1097/01.ehx.0000424169.63765.ac

Cited by 19 publications

(22 citation statements)

References 20 publications

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“…This decrease could be attributed to the decline in levels of T3 and T4 that have a potential effect on the metabolism and homeostasis in animal body. Also, certain study referred to the previous result of impairment in energy metabolic processes along with a decrease in the basal metabolic rate [34] . Moreover, a significant decline in the body weight gain was observed in the rats that have been treated with the different doses of Spirulina compared to those of control rats.…”

Section: Discussionmentioning

confidence: 99%

Impact of Spirulina on Propylthiouracil - Induced Hypothyroidism in Albino Rats, a histological, immunohistochemical and biochemical approach

El-Tantawi

Abo-Zeid

2019

Egyptian Journal of Histology

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Background: Hypothyroidism is a decrease in the production of the thyroid hormones and leads to gland dysfunction. Spirulina used as an antioxidant and supposed as antihypothyroidic agent. Objective: This study was carried out to investigate the impact of Spirulina on PTU-induced hypothyroidism in rats. Materials and Methods: The rats were divided into six groups, control group (G1), hypothyroid group (G2), SP-500 treated group (G3), SP-1000 treated group (G4), PTU+SP-500 treated group (G5) and PTU+SP-1000 treated group (G6). Thyroid gland was examined using biochemical, histological, and immunohistochemical studies. Duration of treatments were for 14 days. Results: A significant decline in the body weight gain was exhibited. Biochemically, a significant decrease in T3 and T4 hormone levels in the PTU-group and a substantial increase in groups treated with Spirulina alone. While PTU+ Spirulina treated groups revealed normal hormonal levels more or less similar to the control group. Histological changes such as congestion of the blood capillaries, follicular dilatation, and vacuolar degeneration of some follicular cells were exhibited in hypothyroid group G2 and Spirulina treated-groups (G3andG4). Hyperplastic cells with hyperchromatic nuclei, depleted vacuolated-thyroglobulin, and a significant increase in the epithelial heights and the follicular diameters, were observed. Immunohistochemically, low expression of the proliferative cellular marker KI-67 was expressed in the PTU and PTU+Spirulina treated groups. While negative expression of KI-67 in Spirulina treated groups was recorded. Conclusion: Administration of Spirulina alone displayed signs of hyperactivity on the thyroid gland, but it has a mild protective role in the PTU-induced hypothyroidism groups. Therefore, caution should be used in extrapolating these results to the human being situation within different doses and durations.

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Section: Discussionmentioning

confidence: 99%

Impact of Spirulina on Propylthiouracil - Induced Hypothyroidism in Albino Rats, a histological, immunohistochemical and biochemical approach

El-Tantawi

Abo-Zeid

2019

Egyptian Journal of Histology

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“…Пролиферация интрафолликулярного эпителия ЩЖ с образованием внутрифолликулярных сосочковых структур и признаки гиперемии в строме органа также были обнаружены другими авторами при введении тиомочевины и ее производных, к которым относится ПТУ, крысам, мышам, овцам [16][17][18]. Авторы объясняют данные изменения стимуляцией пролиферативной активности фолликулярного эпителия на фоне низкого уровня Т4 и повышенного уровня ТТГ.…”

Section: результаты и их обсуждениеunclassified

“…3, в), однако наблюдалось утолщение стенок цент ральных вен. Наблюдения предыдущих авторов также выявили венозную гиперемию ЩЖ, легких, печени, селезенки, кожи при введении ПТУ [16,18,19]. До сих пор до конца не ясен механизм, посредством которого ПТУ вызывает васкулит и кровоизлияния, однако известно о ведущей роли аутоимунных процессов в патогенезе данного осложнения [19].…”

Section: результаты и их обсуждениеunclassified

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Pobelensky

Legach

Pobelensky

et al. 2020

PEP

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Исследование выполнено в рамках научно-исследовательской работы «Свойства криоконсервированных первичных культур клеток эндокринных желез неонатальных животных in vitro и in vivo при трансплантации» (№ государственной регистрации 0116U003494). Учреждением, финансирующим исследование, является НАН Украины. Авторы гарантируют ответственность за объективность представленной информации. Авторы гарантируют отсутствие конфликта интересов и собственной финансовой заинтересованности. Рукопись поступила в редакцию 20.11.2019.

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“…У попередній роботі нами було встановлено, що низка гістопатологічних змін у ЩЗ щурів лінії SHR та Вістар при введенні ПТУ включає збільшення маси ЩЗ, висоти фолікулярного епітелію, кількості патологічних фолікулів з сосочковими структурами, зменшенням колоїдного вмісту у фолікулах [22][23][24][25]…”

Section: мета дослідженняunclassified

Comparative results of thyroid cryoablation in SHR and vistar rats

Pobielienskyi

Olefirenko

Pobielienskyi

et al. 2020

The Journal of v. N. Karazin Kharkiv National University, Series "Medicine"

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Introduction. Today, cryosurgery is considered as a promising minimally invasive approach to the treatment of benign nodules of thyroid gland. Blood circulation in the tissue can affect the effectiveness of cryoablation preventing decline of the temperature. This is true for the thyroid gland, which belongs to organs with a high blood circulation rate. The SHR rat can be used for modeling the processes of thyroid cryoablation in people with essential hypertension, which makes it possible to obtain calculated data on reaching the target freezing temperature in patients with arterial hypertension. Aim of the study – make a comparative assessment of histological characteristics of thyroid gland tissue in hypertensive SHR rats and normotensive Wistar rats after cryoablation. Materials and methods. In the experiments, SHR and Wistar rats weighing 250–280 g were used. Cryodestruction of the thyroid gland was carried out in intact animals and rats, which were administrated with a 0.1 % solution of propylthiouracil to induce diffuse thyroid hyperplasia. The operation was performed under general anesthesia. Cryotherapy was carried out for 40 s once on the left lobe of the thyroid gland using a copper cryoprobe, which was cooled to the temperature of liquid nitrogen. On day 21, the animals were taken out of the experiment; the thyroid gland were taken and used for histological studies. Results. It was found that at the site of cryotherapy in gland samples of animals of both lines on day 21, a cone-shaped zone of fibrosis was formed, in which the parenchyma of the gland was replaced by connective tissue with pronounced vascularization and insignificant lymphocytic-histiocytic infiltration. The mean area of fibrosis did not differ significantly between the groups of intact SHR and Wistar rats. After cryoablation of glands with administration of propylthiouracil, a decrease in the area of fibrotic changes was observed: in Wistar rats – by 2 times, in SHR rats – by 3–4 times. Conclusions. Parameters of blood circulation in arterial hypertension did not significantly affect the result of thyroid gland cryoablation in rats. However, after induction of tissue hyperplasia with propylthiouracil, an increase in gland resistance to cryodestruction is observed, which should be taken into account when choosing a cryotherapy regimen.

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Histological and immunohistochemical study of the effect of experimentally induced hypothyroidism on the thyroid gland and bone of male albino rats

Cited by 19 publications

References 20 publications

Impact of Spirulina on Propylthiouracil - Induced Hypothyroidism in Albino Rats, a histological, immunohistochemical and biochemical approach

Impact of Spirulina on Propylthiouracil - Induced Hypothyroidism in Albino Rats, a histological, immunohistochemical and biochemical approach

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Comparative results of thyroid cryoablation in SHR and vistar rats

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