Histological and lectin histochemical studies in the vomeronasal organ of the Korean black goat, Capra hircus coreanae

Yang, Wonjun; Choi, Yu-Mi; Park, Changnam; Lee, Kwang-Hyup; Ahn, Meejung; Kang, Wanchoul; Heo, Seungdam; Kim, Jeong-Tae; Shin, Taekyun

doi:10.1016/j.acthis.2021.151684

Cited by 9 publications

(29 citation statements)

References 35 publications

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“…The VSE of the VNO in Korean native cattle was formed by the prolongations of receptor cells and supporting cells in the superficial layer and scattered basal cells in the basal layer. These defined layers, as well as the neuroepithelium development, are consistent with previous observations in other ungulates, including goats (Yang et al, 2021) and pigs (Park et al, 2012). Consistent with previous studies, nuclei of the vomeronasal basal cells were situated at the basal region of the VSE, while those of the receptor cells were arranged in one or two layers with distinct nucleoli.…”

Section: Discussionsupporting

confidence: 92%

“….5 antibody was used to stain cattle VNOs as a marker of neurons and neuroendocrine cells (Thompson et al, 1983). PGP9.5 immunoreactivities were strongly expressed in neonatal and adult VSEs, as well as in some not uniform (Dennis et al, 2003), and immunopositivity has been observed in goats (Yang et al, 2021), but not in camels (Ibrahim et al, 2015). Although conditions such as age differ for each animal in these studies, additional studies are needed to clarify the role of PGP9.5 in relation to sensory neurons in VNO.…”

Section: Discussionmentioning

confidence: 96%

“…Numerous studies have been conducted on the anatomy of the VNO in cattle (Minett, 1925; Taniguchi and Mikami, 1985; Adams, 1986;Salazar et al, 1995;Salazar et al, 1997) (Yang et al, 2021), sheep (Ibrahim et al, 2014), pigs (Salazar et al, 2000), horses (Lee et al, 2003), and camels (Ibrahim et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Both protein gene product9.5 (PGP9.5; neuron and neuroendocrine cell marker) and olfactory marker protein (OMP; a mature olfactory receptor cell marker) are expressed in VNO receptor neurons (Thompson et al, 1983;Buiakova et al, 1994). Previous studies have shown that PGP9.5 and OMP are expressed in most VNO receptor cells of the VSE and in some isolated cells of the VNSE in various mammals, including mice (Lin et al, 2008), goats (Yang et al, 2021), camels (Ibrahim et al, 2015), horses (Lee et al, 2016), non-human primates (Dennis et al, 2004), and humans (Johnson et al, 1995).…”

Section: Histology and Lectin Histochemistry In The Vomeronasal Organ Of Korean Native Cattle Bos Taurus Coreanaementioning

confidence: 99%

“…In chemosensory systems, lectin histochemistry has commonly been utilized to discriminate terminal carbohydrate residues (Salazar et al, 2001). The carbohydrate (lectin-binding) moiety is expressed in the VNOs of various mammals, including rats (Mendoza and Kuhnel, 1991), mice (Barrios et al, 2014), rabbits (Villamayor et al, 2018), foxes (Ortiz-Leal et al, 2020), marmosets (Nakajima et al, 1998), goats (Yang et al, 2021), pigs (Salazar et al, 2004;Park et al, 2012), sheep (Ibrahim et al, 2014), roe deer (Park et al, 2014), and horses (Lee et al, 2016). However, little is known about the expression of PGP9.5, OMP, and diverse lectin-binding carbohydrates in various cell types and structures in the VNO of cattle even though the morphological features of the cattle VNO are well-characterized (Taniguchi and Mikami, 1985;Adams, 1986;Salazar et al, 1995;Salazar et al, 1997;Salazar et al, 2008).…”

Section: Histology and Lectin Histochemistry In The Vomeronasal Organ Of Korean Native Cattle Bos Taurus Coreanaementioning

confidence: 99%

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Histology and lectin histochemistry in the vomeronasal organ of Korean native cattle, Bos taurus coreanae

Jang

Kim

et al. 2021

J Anim Reprod Biotechnol

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The vomeronasal organ (VNO) is critical for reproduction and social behavior in ruminants, including cattle. The present study examined the structure of the VNO and its epithelial cells in neonatal and adult Korean native cattle (Hanwoo), Bos taurus coreanae, using immunohistochemistry and lectin histochemistry. Histologically, the VNO comprised two types of epithelia: medial vomeronasal sensory (VSE) and lateral vomeronasal non-sensory epithelia (VNSE). Numerous blood vessels and nerve bundles were observed within the vomeronasal cartilage encased lamina propria. Immunohistochemistry revealed high expression level of protein gene product9.5 and moderate expression level of olfactory marker protein in the neuroreceptor cells of the VSE and occasionally in some ciliated cells of the VNSE in both neonates and adults. The properties of the glycoconjugates in the VNO were investigated using 21 lectins, most of which were expressed at varied intensities in the VSE and VNSE, as well as in the lamina propria. Several lectins exhibited variations in their intensities and localization between neonatal and adult VNOs. This study is the first descriptive lectin histochemical examination of the VNO of Korean native cattle with a focus on lectin histochemistry, confirming that the VNO of Korean native cattle is differentiated during postnatal development.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Histology and Lectin Histochemistry In The Vomeronasal Organ Of Korean Native Cattle Bos Taurus Coreanaementioning

confidence: 99%

Section: Histology and Lectin Histochemistry In The Vomeronasal Organ Of Korean Native Cattle Bos Taurus Coreanaementioning

confidence: 99%

See 3 more Smart Citations

Histology and lectin histochemistry in the vomeronasal organ of Korean native cattle, Bos taurus coreanae

Jang

Kim

et al. 2021

J Anim Reprod Biotechnol

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Immunohistological study of the unexplored vomeronasal organ of an endangered mammal, the dama gazelle (Nanger dama)

Torres

Ortiz‐Leal

Ferreiro³

et al. 2023

Microscopy Res & Technique

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Dama gazelle is a threatened and rarely studied species found primarily in northern Africa. Human pressure has depleted the dama gazelle population from tens of thousands to a few hundred individuals. Since 1970, a founder population consisting of the last 17 surviving individuals in Western Sahara has been maintained in captivity, reproducing naturally. In preparation for the future implementation of assisted reproductive technology, certain aspects of dama gazelle reproductive biology have been established. However, the role played by semiochemical‐mediated communications in the sexual behavior of dama gazelle remains unknown due partially to a lack of a neuroanatomical or morphofunctional characterization of the dama gazelle vomeronasal organ (VNO), which is the sensory organ responsible for pheromone processing. The present study characterized the dama gazelle VNO, which appears fully equipped to perform neurosensory functions, contributing to current understanding of interspecies VNO variability among ruminants. By employing histological, lectin‐histochemical, and immunohistochemical techniques, we conducted a detailed morphofunctional evaluation of the dama gazelle VNO along its entire longitudinal axis. Our findings of significant structural and neurochemical transformation along the entire VNO suggest that future studies of the VNO should take a similar approach. The present study contributes to current understanding of dama gazelle VNO, providing a basis for future studies of semiochemical‐mediated communications and reproductive management in this species.Research Highlights This exhaustive immunohistological study of the vomeronasal organ (VNO) of the dama gazelle provides the first evidence of notable differences in the expression of neuronal markers along the rostrocaudal axis of the VNO. This provides a morphological basis for the implementation of pheromones in captive populations of dama gazelle.

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Exploring the vomeronasal organ in an endangered antelope species

Torres¹,

Ortiz‐Leal²,

Ferreiro³

et al. 2023

Preprint

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The dama gazelle is a threatened and scarcely studied species of northern Africa. Human pressure has depleted the population of dama gazelles from tens of thousands to a few hundred individuals. Since 1970, after deriving from a founder population of the last 17 surviving individuals in the Western Sahara, it has been reproduced naturally in captivity. Although certain aspects of the reproductive biology of the dama gazelle have been established in preparation for the future implementation of assisted reproductive technology there is a lack of information regarding the role of semiochemical-mediated communication in the sexual behavior of the dama gazelle. This is partially due to the lack of a neuroanatomical and morphofunctional characterization of the dama gazelle vomeronasal organ (VNO); the sensory organ responsible for the processing of pheromones. This study aims to determine the presence in the dama gazelle of a VNO fully equipped to carry out its neurosensory function and to contribute to the understanding of the interspecific variability of the VNO of ruminants. Employing histological, lectin-histochemical and immunohistochemical techniques we have performed a detailed morphofunctional evaluation of the dama gazelle VNO along its entire longitudinal axis. The findings suggest that studies of the VNO should take a similar approach, as there are significant structural and neurochemical transformations that the organ exhibits as a whole. This study contributes to the understanding of the VNO in dama gazelles and provides a basis for future studies on semiochemical-mediated communication and reproductive management of this species.

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Histological and lectin histochemical studies in the vomeronasal organ of the Korean black goat, Capra hircus coreanae

Cited by 9 publications

References 35 publications

Histology and lectin histochemistry in the vomeronasal organ of Korean native cattle, Bos taurus coreanae

Histology and lectin histochemistry in the vomeronasal organ of Korean native cattle, Bos taurus coreanae

Immunohistological study of the unexplored vomeronasal organ of an endangered mammal, the dama gazelle (Nanger dama)

Exploring the vomeronasal organ in an endangered antelope species

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