Histological and miRNAs postmortem changes in incisional wound

Ibrahim, Samah F.; Ali, Musab A. M.; Basyouni, Hoda; Rashed, Laila Ahmed; Amer, Eman; El-Kareem, Dalia Abd

doi:10.1186/s41935-019-0141-7

Cited by 16 publications

(23 citation statements)

References 23 publications

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“…According to world literature sources, experts in practice use mostly visual and descriptive methods, and then to clarify the results -histological, histochemical and immunohistochemical methods of research. (Siddiqui et al, 2016;Ibrahim et al, 2019). Siddiqui et al described the possibility of using a histopathological method to establish a causal relationship between the time of injury and the time of death.…”

Section: Resultsmentioning

confidence: 99%

Spectrophotopolarimetry as a Method for Determining the Time Dynamics of Changes in the Morphological Structure of Muscle Tissue

Harazdiuk

Kozan

Kryvetskyi

et al. 2021

PMP

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Introduction: The article presents the current state of issues regarding the diagnosis of pathological conditions for forensic medical experts, in particular the study of normal muscle tissue and in the presence of hemorrhages. Particular attention is paid to biophysical research methods with evidence-based accuracy, objectivity, reproducibility, and rapid results. Objectives: The purpose of the research was to study the possibilities of using spectrophotopolyarimetric methods to analyze and determine the relationship of morphological structure of biological tissues with the temporal dynamics of changes in their optical parameters, to establish the possibility of differentiating necrotic changes and signs of damage by applying Mueller matrices of human muscle tissue samples. Results: During the study, we found a number of features and patterns of change in the properties of the laser beam as a result of passing through biological tissue. Polarization-correlation methods provide new, objective information about the dynamics of change in laser polarimetric imaging of morphological structure of biological tissues. By analyzing the statistical distributions of the ellipticity of polarization of laser images of normal human muscle tissue and in the presence of hemorrhages, the possibility of identifying the relationship between the development of statistically significant changes with increasing time of death and the possibility of differentiating them are considered. Conclusion: Based on these results, we can conclude that employing laser polarization methods will allow us to open new doors in the study of the biological tissues of the human body. The results showed the effectiveness of the studied methodology and the prospect of further research in this direction.

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Section: Resultsmentioning

confidence: 99%

Spectrophotopolarimetry as a Method for Determining the Time Dynamics of Changes in the Morphological Structure of Muscle Tissue

Harazdiuk

Kozan

Kryvetskyi

et al. 2021

PMP

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“…In the rat's brain, a slight downregulation of miR-16 was found throughout the 24 h of PMI (Nagy et al, 2015). On the contrary, miR-124a, miR-205, and miR-21 were found upregulated within the first 24 h of PMI in brain and skin (Nagy et al, 2015;Ibrahim et al, 2019). These studies and our results suggest that some miRNAs could be actively involved in the decomposition process, possibly regulating the expression of other genes, rather than being inert molecules which heavily resist degradation.…”

Section: Discussionmentioning

confidence: 99%

Dysregulation of miR-381-3p and miR-23b-3p in skeletal muscle could be a possible estimator of early post-mortem interval in rats

Martínez-Rivera

Cárdenas-Monroy

Millan-Catalan

et al. 2021

PeerJ

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Background The post-mortem interval (PMI) is the time elapsed since the dead of an individual until the body is found, which is relevant for forensic purposes. The miRNAs regulate the expression of some genes; and due to their small size, they can better support degradation, which makes them suitable for forensic analysis. In the present work, we evaluated the gene expression of miR-381-3p, miR-23b-3p, and miR-144-3p in skeletal muscle in a murine model at the early PMI. Methods We designed a rat model to evaluate the early PMI under controlled conditions. This model consisted in 25 rats divided into five groups of rats, that correspond to the 0, 3, 6, 12 and 24 hours of PMI. The 0 h-PMI was considered as the control group. Muscle samples were taken from each rat to analyze the expression of miR-381-3p, miR-23b-3p, and miR-144-3p by quantitative RT-PCR. The gene expression of each miRNA was expressed as Fold Change (FC) and compared among groups. To find the targets of these miRNAs and the pathways where they participate, we performed an in-silico analysis. From the gene targets of miR-381-3p identified in the silico analysis, the EPC1 gene was selected for gene expression analysis by quantitative RT-PCR in these samples. Also, to evaluate if miR-381-3p could predict the early PMI, a mixed effects model was calculated using its gene expression. Results An upregulation of miR-381-3p was found at 24 h-PMI compared with the control group of 0 h-PMI and (FC = 1.02 vs. FC = 1.96; p = 0.0079). This was the opposite for miR-23b-3p, which had a down-regulation at 24 h-PMI compared to 0 h-PMI (FC = 1.22 vs. FC = 0.13; p = 0.0079). Moreover, the gene expression of miR-381-3p increased throughout the first 24 h of PMI, contrary to miR-23b-3p. The targets of these two miRNAs, participate in biological pathways related to hypoxia, apoptosis, and RNA metabolism. The gene expression of EPC1 was found downregulated at 3 and 12 h of PMI, whereas it remained unchanged at 6 h and 24 h of PMI. Using a multivariate analysis, it was possible to predict the FC of miR-381-3p of all but 6 h-PMI analyzed PMIs. Discussion The present results suggest that miR-23b-3p and miR-381-3p participate at the early PMI, probably regulating the expression of some genes related to the autolysis process as EPC1 gene. Although the miR-381-3p gene expression is a potential estimator of PMI, further studies will be required to obtain better estimates.

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“…In the rat's brain, a slight downregulation of miR-16 was found throughout the 24 h of PMI (Nagy et al, 2015). On the contrary, miR-124a, miR-205, and miR-21 were found upregulated within the first 24 h of PMI in brain and skin (Nagy et al, 2015;Ibrahim et al, 2019). These studies and our results suggest that some miRNAs could be actively involved in the decomposing process, possibly regulating the expression of other genes, rather than being inert molecules which heavily resist degradation.…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #2 of "Dysregulation of miR-381-3p and miR-23b-3p in skeletal muscle could be a possible estimator of early post-mortem interval in rats (v0.1)"

2021

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Histological and miRNAs postmortem changes in incisional wound

Cited by 16 publications

References 23 publications

Spectrophotopolarimetry as a Method for Determining the Time Dynamics of Changes in the Morphological Structure of Muscle Tissue

Spectrophotopolarimetry as a Method for Determining the Time Dynamics of Changes in the Morphological Structure of Muscle Tissue

Dysregulation of miR-381-3p and miR-23b-3p in skeletal muscle could be a possible estimator of early post-mortem interval in rats

Peer Review #2 of "Dysregulation of miR-381-3p and miR-23b-3p in skeletal muscle could be a possible estimator of early post-mortem interval in rats (v0.1)"

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