Histological and molecular changes in gill and liver of fish (Astyanax lacustris Lütken, 1875) exposed to water from the Doce basin after the rupture of a mining tailings dam in Mariana, MG, Brazil

Macêdo, Anderson Kelvin Saraiva; Santos, Keiza Priscila Enes dos; Brighenti, Ludmila Silva; Windmöller, Cláudia Carvalhinho; Barbosa, Francisco A. R.; Ribeiro, Rosy Iara Maciel de Azambuja; Santos, Hélio Batista dos; Thomé, Ralph Gruppi

doi:10.1016/j.scitotenv.2020.139505

Cited by 63 publications

(30 citation statements)

References 70 publications

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“…Our statement agreed with Samanta et al Rodrigues, et al and Huang et al the authors mentioned that structural changes are relevant to oxidative stress 78,82,84 . Cellular anomalies could be an indication of oxidative damage in the tissue or biochemical changes or physiological disturbances 68,78,85 …”

Section: Discussionsupporting

confidence: 88%

“…Necrosis could occur in the tissue due to inhibition of enzymes and damages in the cellular membrane integrity. Moreover, fish gills anomalies could occur as a compensatory adjustment to maintain ions homeostasis 68 . Gills tissue morphological changes could be considered as the direct toxic action of chemicals since it directly contacts with the external environment 69 .…”

Section: Discussionmentioning

confidence: 99%

“…Occurrence of hepatocyte vacuoles is to specify the disruption between the rate of synthesis and release of substances in the parenchymal cells 76 . The authors Macêdo et al mentioned that hepatocyte vocalization indicates inhibition of protein synthesis, energy depletion, microtubule disintegration, or changes in substrate utilization 68 . Vacuolation indicates tissue damage and the release of hepatic enzymes due to apoptosis and RBCs membranes devastation 77 .…”

Section: Discussionmentioning

confidence: 99%

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Responses of Cirrhinus mrigala to second‐generation fluoroquinolone (ciprofloxacin) toxicity: Assessment of antioxidants, tissue morphology, and inorganic ions

Ramesh

Sujitha

Anila

et al. 2020

Environmental Toxicology

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Ciprofloxacin drugs are a second‐generation fluoroquinolone highly prescribed medication against various bacterial infections in human and aquaculture practices. These drugs are chemically designed to persist in the body long enough to achieve target objectives. Extensive usage has resulted in ciprofloxacin becoming a ubiquitous contaminant in the environment. Unfortunately, the ecotoxicological profiles for ciprofloxacin are scanty. This study was aimed to assess the ecotoxicity of ciprofloxacin at environmentally relevant concentrations (1 μg/L, and 1.5 μg/L) to a cultivable fish Cirrhinus mrigala. Responses of antioxidant enzymes, histological anomalies, and inorganic ion levels were studied. SOD activity in gill, liver, and kidney tissues was elevated in ciprofloxacin‐exposed groups when compared with the control group. CAT activity was predominantly decreased in ciprofloxacin treated groups relative to the control group. GST activity in the ciprofloxacin treated groups was increased (except kidney tissues [Treatment I (1 μg/L)], and gill tissues fifteenth day) significantly (p < .05). The LPO level was elevated in the ciprofloxacin treatment groups throughout the study period (except Treatment II (1.5 μg/L) tenth day in kidney tissues). A series of histological anomalies were noticed in the gill, liver, and kidney tissues of the ciprofloxacin treated groups. Ciprofloxacin exposure caused a significant decrease of sodium, potassium, and chloride levels in the plasma of C. mrigala. A parallel among an imbalanced oxidative defense system, tissue structural changes, and alterations of plasma inorganic ions could be considered as a reliable biomarker for antibiotic toxicity study. This study could be a primary platform for further toxicity studies to understand the potential molecular impacts and adverse effects of ciprofloxacin on aquatic organisms.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Responses of Cirrhinus mrigala to second‐generation fluoroquinolone (ciprofloxacin) toxicity: Assessment of antioxidants, tissue morphology, and inorganic ions

Ramesh

Sujitha

Anila

et al. 2020

Environmental Toxicology

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“…Although GSH levels may vary among fish species, the species captured in the Rio Doce estuary exhibited higher GSH expression when compared to fish from uncontaminated freshwater ecosystems upstream ( Weber et al, 2020 ). In addition, fish species from contaminated freshwater ecosystems upstream exhibited similar oxidative stress effects, with higher biomarker levels in addition to internal tissue degeneration ( Weber et al, 2020 ; Macêdo et al, 2020 ). The GSH expression in fish captured in the estuary suggests that local estuarine ecosystem health has also been severely compromised by trace metal contamination, with possible sub-optimal conditions for the development of fish species ( Andrades et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Contamination and oxidative stress biomarkers in estuarine fish following a mine tailing disaster

Gabriel

Hauser‐Davis

Soares

et al. 2020

PeerJ

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Background The Rio Doce estuary, in Brazil, was impacted by the deposition of iron mine tailings, caused by the collapse of a dam in 2015. Based on published baseline datasets, the estuary has been experiencing chronic trace metal contamination effects since 2017, with potential bioaccumulation in fishes and human health risks. As metal and metalloid concentrations in aquatic ecosystems pose severe threats to the aquatic biota, we hypothesized that the trace metals in estuarine sediments nearly two years after the disaster would lead to bioaccumulation in demersal fishes and result in the biosynthesis of metal-responsive proteins. Methods We measured As, Cd, Cr, Cu, Fe, Mn, Pb, Se and Zn concentrations in sediment samples in August 2017 and compared to published baseline levels. Also, trace metals (As, Cd, Cr, Cu, Fe, Hg, Mn, Pb, Se and Zn) and protein (metallothionein and reduced glutathione) concentrations were quantified in the liver and muscle tissues of five fish species (Cathorops spixii, Genidens genidens, Eugerres brasilianus, Diapterus rhombeus and Mugil sp.) from the estuary, commonly used as food sources by local populations. Results Our results revealed high trace metal concentrations in estuarine sediments, when compared to published baseline values for the same estuary. The demersal fish species C. spixii and G. genidens had the highest concentrations of As, Cr, Mn, Hg, and Se in both, hepatic and muscle, tissues. Trace metal bioaccumulation in fish was correlated with the biosynthesis of metallothionein and reduced glutathione in both, liver and muscle, tissues, suggesting active physiological responses to contamination sources. The trace metal concentrations determined in fish tissues were also present in the estuarine sediments at the time of this study. Some elements had concentrations above the maximum permissible limits for human consumption in fish muscles (e.g., As, Cr, Mn, Se and Zn), suggesting potential human health risks that require further studies. Our study supports the high biogeochemical mobility of toxic elements between sediments and the bottom-dwelling biota in estuarine ecosystems.

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“…When exposed to sublethal concentrations of atrazine, fish showed an increase in oxidative stress, behavioral changes, endocrine disruption, reduced fertilization rate, and increased frequency of histopathological alterations (Adeyemi et al, 2015; Bautista et al, 2018; Hayes et al, 2011; Jin et al, 2010; Khoshnood et al, 2015; Liu et al, 2016; Oliveira et al, 2018; Owolabi & Omotosho, 2017). The study of histological alterations of various organs in fish has been used as a marker to assess the effect of contaminants in aquatic environments (Badroo et al, 2020; Macêdo et al, 2020; Nassar et al, 2020). These biomarkers might be examined in key organs such as the gills, liver, and kidney, that have vital functions, for example, respiration, excretion, accumulation, and biotransformation of xenobiotics (Hinton & Lauren, 1990; Rey et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Can the exposure system adopted influence the results of the atrazine toxicity in hepatic tissue of fish?

et al. 2021

Self Cite

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The widespread use of atrazine, a herbicide used to control weeds, has contributed to the increased contamination of aquatic environments. To assess the toxicological effects of a xenobiotic on a nontarget organism in the laboratory, different models of toxicological exposure systems have been widely used. Therefore, the aim of this study was to evaluate and compare the action of sublethal concentrations of atrazine on the hepatic histology of Oreochromis niloticus, considering two models of exposure: static (where atrazine was only added once) and semi-static (where atrazine was periodically renewed). Fish were exposed to a concentration of 2 ppm atrazine for 15 days, which was verified by high-performance liquid chromatography. The livers were stained with hematoxylin and eosin and histopathological data were collected. In addition, they were submitted to immunohistochemistry for inducible nitric oxide synthase (iNOS). A maximum variation of 45% (static) and 12.5% (semi-static) was observed between the observed and nominal atrazine concentration. Nuclear and cytoplasmic changes were observed in both experimental models. Hepatocytes from the livers of the static system showed a degenerative appearance, while in the semi-static system, intense cytoplasmic vacuolization and necrosis were observed. iNOS positive cells were identified only in macrophages in the hepatocytes of fish in the semi-static system. These results directly showed how the choice of exposure system can influence the results of toxicological tests. However, future analysis investigating the by-products and nitrogen products should be carried out since the histopathological findings revealed the possibility of these compounds serving as secondary contamination routes.

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Histological and molecular changes in gill and liver of fish (Astyanax lacustris Lütken, 1875) exposed to water from the Doce basin after the rupture of a mining tailings dam in Mariana, MG, Brazil

Cited by 63 publications

References 70 publications

Responses of Cirrhinus mrigala to second‐generation fluoroquinolone (ciprofloxacin) toxicity: Assessment of antioxidants, tissue morphology, and inorganic ions

Responses of Cirrhinus mrigala to second‐generation fluoroquinolone (ciprofloxacin) toxicity: Assessment of antioxidants, tissue morphology, and inorganic ions

Contamination and oxidative stress biomarkers in estuarine fish following a mine tailing disaster

Can the exposure system adopted influence the results of the atrazine toxicity in hepatic tissue of fish?

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