Histological assessment of cellular changes in gingival epithelium in ante-mortem and post-mortem specimens

Pradeep, G L; Uma, K.; Prakash, Swayam; Prakash, Nilima

doi:10.4103/0974-2948.60375

Cited by 21 publications

(28 citation statements)

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“…For gingival tissue, PMI has been related to different histological changes (Pradeep et al. ; Yadav et al. ; Mahalakshmi et al.…”

Section: Introductionmentioning

confidence: 99%

“…Over the decades, histological and immunohistochemical analyses of different post mortem tissues have become increasingly important, as they might provide additional hints that may be useful in determining the time interval after death (Thaik-Oo et al 2002;Dettmeyer, 2011). For gingival tissue, PMI has been related to different histological changes (Pradeep et al 2009;Yadav et al 2015;Mahalakshmi et al 2016), but the associated post mortem immunohistochemical changes seem to be overlooked.…”

Section: Introductionmentioning

confidence: 99%

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HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

et al. 2018

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Estimating the post mortem interval (PMI) is still a crucial step in Forensic Pathology. Although several methods are available for assessing the PMI, a precise estimation is still quite unreliable and can be inaccurate. The present study aimed to investigate the immunohistochemical distribution and mRNA expression of hypoxia inducible factor (HIF‐1α) in post mortem gingival tissues to establish a correlation between the presence of HIF‐1α and the time since death, with the final goal of achieving a more accurate PMI estimation. Samples of gingival tissues were obtained from 10 cadavers at different PMIs (1–3 days, 4–5 days and 8–9 days), and were processed for immunohistochemistry and quantitative reverse transcription‐polymerase chain reaction. The results showed a time‐dependent correlation of HIF‐1α protein and its mRNA with different times since death, which suggests that HIF‐1α is a potential marker for PMI estimation. The results showed a high HIF‐1α protein signal that was mainly localized in the stratum basale of the oral mucosa in samples collected at a short PMI (1–3 days). It gradually decreased in samples collected at a medium PMI (4–5 days), but it was not detected in samples collected at a long PMI (8–9 days). These results are in agreement with the mRNA data. These data indicate an interesting potential utility of Forensic Anatomy‐based techniques, such as immunohistochemistry, as important complementary tools to be used in forensic investigations.

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“…For gingival tissue, PMI has been related to different histological changes (Pradeep et al. ; Yadav et al. ; Mahalakshmi et al.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

et al. 2018

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“…The cell thus undergoes a process of autolysis. [ 7 ] Histomorphologically, the autolysis represents the intravital or postmortal disintegration of living structures and biochemically corresponds to a loss in the system of metabolic balance with demotion of the metabolic substance which results in energy and material loss. Autolysis matches with the activity of certain enzymes, called autolytical enzymes, proved to exist in lysosomes of living cells, which after death lead to the destruction of their own cell components.…”

Section: Discussionmentioning

confidence: 99%

“…These changes include autolysis, algor mortis, rigor mortis, livor mortis, postmortem clotting putrefaction, and adipocere. [ 7 ] Attempts have also been made to determine time passed since death by studying biochemical changes in blood, cerebrospinal fluid, and intraocular fluids. The biochemical methods have been found to be of not much use once the decomposition changes start.…”

Section: Introductionmentioning

confidence: 99%

Documentation of postmortem changes in salivary gland architecture and staining characteristics

Agarwal

Chaudhary

Gawande

et al. 2016

J Forensic Dent Sci

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Context:Estimation of time passed since death continues to be a major problem for the forensic pathologist and its determination plays an important and vital role in medico-legal cases. The histological studies on various tissues after death have been mostly confined to single organ or tissue by individual workers at different atmospheric conditions.Aims:The aim of this study is to determine the best rehydrating solution for dehydrated tissues in postmortem examination.Settings and Design:This study was specific to salivary gland tissues and certain pattern of changes were determined during postmortem time intervals using hematoxylin and eosin stain and special stains like mucicarmine and alcian blue.Materials and Methods:The study was divided into two groups. (1) Group A: Normal tissue samples (twenty normal salivary gland tissue samples left without fixation for varying periods of time). (2) Group B: Control group (twenty normal salivary gland tissue samples immediately fixed in formalin). The three different rehydrating agents used in this study were glycerol, normal saline and modified Ruffer solution.Statistical Analysis Used:Not required.Results:Modified Ruffer solution is the best when compared to glycerol and normal saline for rehydration of dehydrated tissues.Conclusions:Thus in our study we conclude that the tissue which had been dehydrated at the crime scene for a fairly long period showed better rehydration with modified Ruffer solution and yield good cellular and nuclear details.

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“…Histological examination of gingival tissues procured from postmortem and antemortem samples at different time intervals revealed that decomposition process is initiated within 10 h after death and other cellular changes occur subsequently. [ 5 ] The use of oral epithelium cells harvested by pressure application of a toothbrush to assess the minute qualities of DNA of individuals followed by gender identification by sex determining region-Y (SRY) gene amplification was carried out using real-time polymerase chain reaction. The authors claimed that this was a valuable and sensitive tool and functional amounts of DNA could suffice from SRY gene amplification for human gender identification.…”

Section: Assesment Of Gingival Epitheliummentioning

confidence: 99%

Forensic dentistry: Adding a perio 'scope' to it!

Gattani¹,

Deotale²

2016

J Indian Soc Periodontol

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Human identification during mass disasters using the stereotyped techniques such as fingerprint analysis is a cumbersome task. With a view to overcome this predicament, dental evidence of the deceased has gained importance in the recent years. Various branches of dentistry have augmented forensic research through innumerable scientific contributions. The following narrative review of literature aims to summarize the findings of studies that reported periodontal associations or potential associations that could play a role in forensic research. It is designed to inspire the “perio-society” to lend a helping hand to forensic odontology.

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Histological assessment of cellular changes in gingival epithelium in ante-mortem and post-mortem specimens

Cited by 21 publications

References 1 publication

HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

Documentation of postmortem changes in salivary gland architecture and staining characteristics

Forensic dentistry: Adding a perio 'scope' to it!

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