Histological assessment of regenerating plants at callus, shoot organogenesis and plantlet stages during the in vitro micropropagation of Asparagus cochinchinensis

Kim, Yonggoo; Okello, Denis; Yang, Sungyu; Komakech, Richard; Rahmat, Endang; Kang, Young Min

doi:10.1007/s11240-020-01967-3

Cited by 14 publications

(16 citation statements)

References 16 publications

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“…Koroch et al (2002) observed that an increased concentration of NAA resulted in less shoot organogenesis and more callus multiplication. Regeneration responses to PGRs vary depending on the species, as observed by Kim et al (2021).…”

Section: Discussionmentioning

confidence: 95%

“…The presence of compact, small-sized, and isodiametric cells with dense cytoplasm cells in the callus tissue indicated that such regions of the callus tissue were embryogenic (Binte Mostafiz and Wagiran, 2018;Kim et al, 2021). According to the study by Vega et al (2009), cells with dense cytoplasm and abundant starch granules are metabolically active and can undergo dedifferentiation, forming embryogenic calli.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we observed that the MS plant culture medium supplemented with 1.0 mg/L BAP and 0.05 mg/L NAA was very effective to induce shoot regeneration from calli from both root and leaf explants of A. africana. The presence of cytokinins and auxins is essential for callus differentiation and regeneration in plants (Kim et al, 2021). In fact, a combination of NAA and BAP has been shown to induce high shoot organogenic potential in calli of many species in the family Asteraceae such as Echinacea purpurea (Koroch et al, 2002), E. scaber (Abraham and Thomas, 2015), Dendranthema grandiflorum (Khandakar et al, 2014), Carthamus tinctorius (Ghasempour et al, 2014), and Eclipta alba (Sharma et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

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Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

et al. 2021

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The medicinal plant, Aspilia africana, has been traditionally used in several African countries to treat many diseases such as tuberculosis, cough, inflammation, malaria, osteoporosis, and diabetes. In this study, we developed a protocol for in vitro propagation of A. africana using indirect shoot organogenesis from leaf and root explants of in vitro-grown seedlings and assessed the tissues at different developmental stages. The highest callus induction (91.9 ± 2.96%) from leaf explants was in the Murashige and Skoog (MS) medium augmented with 1.0 mg/L 6-Benzylaminopurine (BAP) and 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) while from root explants, the highest callus induction (92.6 ± 2.80%) was in the same plant tissue culture medium augmented with 0.5 mg/L BAP and 1.0 mg/L 2,4-D. The best shoot regeneration capacity from leaf-derived calli (i.e., 80.0 ± 6.23% regeneration percentage and 12.0 ± 6.23 shoots per callus) was obtained in medium augmented with 1.0 mg/L BAP and 0.05 mg/L α-Naphthaleneacetic acid (NAA); the best regeneration capacity for root-derived calli (i.e., 86.7 ± 6.24% shoot regeneration percentage and 14.7 ± 1.11 shoots per callus) was obtained in the MS medium augmented with 1.0 mg/L BAP, 0.05 mg/L NAA, and 0.1 mg/L Thidiazuron (TDZ). Regenerated plantlets developed a robust root system in 1/2 MS medium augmented with 0.1 mg/L NAA and had a survival rate of 93.6% at acclimatization. The in vitro regenerated stem tissue was fully differentiated, while the young leaf tissue consisted of largely unorganized and poorly differentiated cells with large intercellular airspaces typical of in vitro leaf tissues. Our study established a protocol for the indirect regeneration of A. africana and offers a basis for its domestication, large-scale multiplication, and germplasm preservation. To the best of our knowledge, this is the first study to develop an indirect regeneration protocol for A. africana and conduct anatomical assessment through the different stages of development from callus to a fully developed plantlet.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

et al. 2021

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“…Cytokinins generally facilitate shoot multiplication and elongation in plants ( Kim et al, 2021 ). Previous studies on in vitro regeneration from nodal explants of a number of species in the Asteraceae family indicated maximum shoot proliferation when plant tissue culture media were supplemented with only cytokinin(s) without auxins for instance, Stevia rebaudiana ( Ahmed et al, 2007 ; Rafiq et al, 2007 ; Thiyagarajan and Venkatachalam, 2012 ), Artemisia vulgaris ( Sujatha and Kumari, 2008 ), Spilanthes acmella ( Singh and Chaturvedi, 2010 ; Kurian and Thomas, 2015 ), Eclipta alba ( Borthakur et al, 2000 ), and Spilanthes calva ( Razaq et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, during acclimatization, these plant parts grew thicker and larger ( Shekhawat and Manokari, 2018 ). The presence of well-developed and fully differentiated plant tissues may be related to their functionality ( Kim et al, 2021 ). Differentiated tissues, such as vascular bundles in roots and stems, indicate that these organs were fully developed and functional.…”

Section: Discussionmentioning

confidence: 99%

An in vitro Propagation of Aspilia africana (Pers.) C. D. Adams, and Evaluation of Its Anatomy and Physiology of Acclimatized Plants

et al. 2021

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Aspilia africana (Pers.) C. D. Adams is an important medicinal plant, that has been used as traditional medicine in many African countries for the treatment of various health problems, including inflammatory conditions, osteoporosis, tuberculosis, cough, measles, diabetes, diarrhea, malaria, and wounds. We developed an efficient and reproducible protocol for in vitro regeneration of A. africana from nodes. We assessed the effects of plant tissue culture media on A. africana growth, cytokinins for in vitro shoot regeneration and proliferation, and auxins for the rooting of regenerated shoots. Furthermore, chlorophyll content, photosynthetic rates, anatomy (leaves, stems, and roots), and Fourier transform near-infrared (FT-NIR) spectra (leaves, stems, and roots) of the in vitro regenerated and maternal A. africana plants were compared. Murashige and Skoog media, containing vitamins fortified with benzylaminopurine (BA, 1.0 mg/l), regenerated the highest number of shoots (13.0 ± 0.424) from A. africana nodal segments. 1-naphthaleneacetic acid (NAA, 0.1 mg/l) produced up to 13.10 ± 0.873 roots, 136.35 ± 4.316 mm length, and was the most efficient for rooting. During acclimatization, the in vitro regenerated A. africana plants had a survival rate of 95.7%, displaying normal morphology and growth features. In vitro regenerated and mother A. africana plants had similar chlorophyll contents, photosynthetic rates, stem and root anatomies, and FT-NIR spectra of the leaf, stem, and roots. The established regeneration protocol could be used for large-scale multiplication of the plant within a short time, thus substantially contributing to its rapid propagation and germplasm preservation, in addition to providing a basis for the domestication of this useful, high-value medicinal plant.

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Development of an efficient micropropagation protocol for Nematanthus wettsteinii using leaf and shoot-tip explants

He,

Qi,

Yang

et al. 2023

In Vitro Cell.Dev.Biol.-Plant

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Histological assessment of regenerating plants at callus, shoot organogenesis and plantlet stages during the in vitro micropropagation of Asparagus cochinchinensis

Cited by 14 publications

References 16 publications

Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

An in vitro Propagation of Aspilia africana (Pers.) C. D. Adams, and Evaluation of Its Anatomy and Physiology of Acclimatized Plants

Development of an efficient micropropagation protocol for Nematanthus wettsteinii using leaf and shoot-tip explants

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