Histological, biochemical and transcriptomic analyses reveal liver damage in zebrafish (Danio rerio) exposed to phenanthrene

Mai, Yongzhan; Peng, Songyao; Li, Haiyan; Lai, Zhigang

doi:10.1016/j.cbpc.2019.108582

Cited by 20 publications

(11 citation statements)

References 96 publications

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“…In fish, the liver is the basic organ for the metabolism of toxic substances, which is vulnerable to the attack of harmful substances (Hook et al, 2018;Rodd et al, 2017;Mai et al, 2019). The attacked liver is prone to damage such as liver sinusoids, liver necrosis, hepatocyte vacuolization, and nuclear enlargement, which is closely related to liver inflammation (Bentivegna et al, 2015;Kerambrun et al, 2012).…”

Section: Histopathological Changesmentioning

confidence: 99%

Biomarker Responses, Gene Expression Alterations, and Histological Changes in Zebrafish (Danio rerio) After In Vivo Exposure to Polychlorinated Diphenyl Ethers

Xiong²,

Shi³

et al. 2022

Front. Physiol.

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Polychlorinated diphenyl ethers (PCDEs) have been detected in various aquatic matrices, which pose potential threats to aquatic ecosystem security. In this work, both micro and macro analysis methods were used to assess the toxicity of PCDEs to zebrafish. Results indicated that after in vivo PCDE exposure, the oxidative stress and related gene of Danio rerio were significantly changed. The higher concentration or longer exposure time could cause more severe oxidative stress in zebrafish tissues. Compared with among the five tested compounds, more obvious changes in the level of oxidative biomarkers of lower chlorinated PCDEs’ (4-mono-CDE and 4,4′-di-CDE) exposure groups were observed. The integrated biomarker response analysis and gene expression results also indicate a similar trend. Histopathological observation suggested that 4,4′-di-CDE could render liver nuclei enlargement and necrosis, hepatocyte vacuolation, and the development inhibition of ovarian cells. Transmission electron microscope photos showed that 4,4′-di-CDE caused organelle damage in the liver and ovary, including the rupture of the endoplasmic reticulum, swelling of mitochondria, and condensation of chromatin in the liver and mitochondria disappeared significantly in the ovary. The degree of damage is enhanced with the increasing exposure doses. In addition, PCDEs also significantly altered vitellogenin content and related gene (vtg1) expression, suggesting that PCDEs may be estrogen endocrine disruptors. Overall, these results provided some valuable toxicological data of PCDEs on aquatic species.

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Section: Histopathological Changesmentioning

confidence: 99%

Biomarker Responses, Gene Expression Alterations, and Histological Changes in Zebrafish (Danio rerio) After In Vivo Exposure to Polychlorinated Diphenyl Ethers

Xiong²,

Shi³

et al. 2022

Front. Physiol.

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“…The discrepancy could also be related to the endpoints itself, since previous studies concluded that the induction responses of phase II systems, such as GST activity, are less uniform and, therefore, less reliable compared to phase I enzymes [68,121]. In this context, while different PAHs have been shown to increase GST activity in fish [33,53,58,92], other studies found that PAHs decreased GST activity [80,98,129], some showed contrary results of both inhibition and induction [116], or even no alterations of GST activity by PAH exposure [68] in liver, gills or kidney of fish.…”

Section: Biotransformation Responsementioning

confidence: 99%

“…Biotransformation biomarkers have been applied in several studies focusing on the toxicity pathways behind PAH exposure in zebrafish [53,64]. Representative for biotransformation phase I oxygenase activity, mRNA levels of cyp1a1 and protein levels of EROD activity have been found to be up-regulated in fish after the exposure to individual PAHs, such as phenanthrene [80,97]. Besides, Arukwe et al [10] observed an increment in cyp1a1 mRNA levels and EROD activity in a comparable experiment to the present study applying the identical oil type and zebrafish.…”

Section: Biotransformation Responsementioning

confidence: 99%

Multilevel responses of adult zebrafish to crude and chemically dispersed oil exposure

Esteban‐Sánchez

Johann

Alemany³

et al. 2021

Environ Sci Eur

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Background The application of chemical dispersants is a common remediation strategy when accidental oil spills occur in aquatic environments. Breaking down the oil slick into small droplets, dispersants facilitate the increase of particulate and dissolved oil compounds, enhancing the bioavailability of toxic oil constituents. The aim of the present work was to explore the effects of water accommodated fractions (WAF) of a naphthenic North Sea crude oil produced with and without the addition of the chemical dispersant FINASOL OSR 52 to adult zebrafish exposed for 3 and 21 d. Fish were exposed to environmentally relevant concentrations of 5% and 25% WAFOIL (1:200) and to 5% WAFOIL+D (dispersant–oil ratio 1:10) in a semi-static exposure setup. Results The chemically dispersed WAF presented a 20-fold increase of target polycyclic aromatic hydrocarbons (PAHs) in the water phase compared to the corresponding treatment without dispersant and was the only treatment resulting in markedly bioaccumulation of PAHs in carcass after 21 d compared to the control. Furthermore, only 5% WAFOIL+D caused fish mortality. In general, the undispersed oil treatments did not lead to significant effects compared to control, while the dispersed oil induced significant alterations at gene transcription and enzyme activity levels. Significant up-regulation of biotransformation and oxidative stress response genes (cyp1a, gstp1, sod1 and gpx1a) was recorded in the livers. For the same group, a significant increment in EROD activity was detected in liver along with significant increased GST and CAT activities in gills. The addition of the chemical dispersant also reduced brain AChE activity and showed a potential genotoxic effect as indicated by the increased frequency of micronuclei in erythrocytes after 21 d of exposure. Conclusions The results demonstrate that the addition of chemical dispersants accentuates the effect of toxic compounds present in oil as it increases PAH bioavailability resulting in diverse alterations on different levels of biological organization in zebrafish. Furthermore, the study emphasizes the importance to combine multilevel endpoints for a reliable risk assessment due to high variable biomarker responses. The present results of dispersant impact on oil toxicity can support decision making for oil spill response strategies.

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“…Previous studies in zebrafish have identified potential genetic biomarkers after exposure to the environmental pollutant phenanthrene. 59 Recent studies have shown that changes in protein expression related to neurodegenerative diseases can be measured in peripheral body fluids such as blood and saliva. 60,61 It has also been shown that damage to the BBB in certain neurodegenerative diseases leads to the movement of biomarkers out of the brain into the blood 62 .…”

Section: Figure 2: Distribution Of Log2fc Observed For Gene Expressio...mentioning

confidence: 99%

Transcriptomics effects of per- and polyfluorinated alkyl substances in differentiated neuronal cells

Running,

Cristobal,

Karageorgiou

et al. 2024

Preprint

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Per- and polyfluorinated alkyl substances (PFAS) are ubiquitous in most environments, accumulate in several tissues, and can adversely affect human health. PFAS have been implicated in neurodegenerative and behavioral disorders. However, the mechanisms through which PFAS accumulation in neurons affects biological function remain unknown. In this study, SH-SY5Y neuroblastoma cells were used to investigate how perfluorooctanoic acid (PFOA), perfluorooctansulfonic acid (PFOS), perfluorodecanoic acid (PFDA) perfluorodecanesulfonic acid (PFDS), 8:2 fluorotelomer sulfate (8:2 FTS) and 8:2 fluorotelomer alcohol (8:2 FTOH) exposures influence neuronal health. After a 30 microM-, 24-hour exposure, cells accumulated up to 800 ng PFAS/mg protein. Transcriptomics analysis of control and PFAS-exposed cells revealed 721 genes were differentially expressed across six treatments (padj < 0.05). Eleven of these differentially expressed genes were observed for all treatments, suggesting that these genes are potential markers for neuronal PFAS exposure. In PFOA-treated cells, we observed multiple downregulated genes are enriched for functions related to synaptic growth and neural function. In contrast, upregulated genes in PFOS, PFDS, FTS, and FTOH-treated cells showed enrichment in functions related to response to hypoxia and amino acid metabolism. Overall, our results highlight specific biological processes that potentially underlie negative effects of PFAS on neuronal health.

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Histological, biochemical and transcriptomic analyses reveal liver damage in zebrafish (Danio rerio) exposed to phenanthrene

Cited by 20 publications

References 96 publications

Biomarker Responses, Gene Expression Alterations, and Histological Changes in Zebrafish (Danio rerio) After In Vivo Exposure to Polychlorinated Diphenyl Ethers

Biomarker Responses, Gene Expression Alterations, and Histological Changes in Zebrafish (Danio rerio) After In Vivo Exposure to Polychlorinated Diphenyl Ethers

Multilevel responses of adult zebrafish to crude and chemically dispersed oil exposure

Transcriptomics effects of per- and polyfluorinated alkyl substances in differentiated neuronal cells

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