Histological Change of Uterus Endometrium and Expression of the Eggshell-related Genes according to Hen Age

Park, Ji Ae; Cho, Eun Jung; Park, Jung Yeon; Sohn, Sea Hwan

doi:10.5536/kjps.2017.44.1.19

Cited by 8 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous study, the amount of telomeric DNA in the uterine endometrial cells also decreased with increasing hen age ( Park, 2018 ). Chronic stimuli such as sustained laying behavior caused fibrosis and atrophy of the endometrium, resulting in a functional decline in the uterus ( Bahr and Palmer, 1989 ; Park et al, 2017 ). The increase in the length of uterine cilia due to aging implies that the regenerative capacity of the cells degraded ( Woudstra and Thomson, 2002 ).…”

Section: Discussionmentioning

confidence: 99%

The Influence of Hen Aging on Eggshell Ultrastructure and Shell Mineral Components

Park

Sohn

2018

Korean J Food Sci Anim Resour

View full text Add to dashboard Cite

The eggshell, which is a complex and highly ordered structure, is very important factor for food safety and egg marketing. This study investigated the changes in eggshell structure and shell components in relationship to hen age. For this study, we examined the histological change of the endometrium of the 30-, 60-, and 72-wk-old commercial layers, and analyzed the ultrastructure and ionic composition of their eggshells. The results showed that histological deformation, fibrosis, atrophy and elimination of micro-villi in the uterus endometrium were found through microscopic observation that was associated with increasing hen age. Concentration of blood-ion components such as Ca2+, Na+, K+, and Cl– ions did not change with age. Along with the results from the ultrastructure analysis of the eggshell, the palisade layer ratio and the density of mammillary knobs were significantly decreased in older hens. In addition, the type B mammillary knobs were frequently observed with increasing hen age. In the mineral element assay from the eggshell, Ca2+, S2–, and Co2+ significantly decreased with increasing hen age, whereas Na+, K+, and V2+ significantly increased. Therefore, the damages of endometrial tissue inhibit the processes of ion transmission and the crystallization of eggshell formation, resulting in a large and non-uniform mammillary knob formation. This means the conditions of endometrial cells affect the formation of the eggshell structure. In conclusion, hen aging causes the weakness of the eggshell and degrades the eggshell quality.

show abstract

Section: Discussionmentioning

confidence: 99%

The Influence of Hen Aging on Eggshell Ultrastructure and Shell Mineral Components

Park

Sohn

2018

Korean J Food Sci Anim Resour

View full text Add to dashboard Cite

show abstract

“…Hence, deteriorations in eggshell ultrastructure and quality in the late phase of production were speculated to be associated with age-related dysregulation of gene expression in uterus. It was supported by the findings that the expression of genes encoding ion transporters and matrix proteins (ATP2A2, SCNN1G, CA2 and ovocalyxin-36) changed with the age of laying hens [ 19 ]. Age-related changes in the expression of matrix proteins (ovalbumin, ovotransferrin and ovocleidin-17) in uterus of laying hens were suggested by their varying concentration in eggshell [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 97%

Uterine transcriptome analysis reveals mRNA expression changes associated with the ultrastructure differences of eggshell in young and aged laying hens

Feng

Zhang

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

Background Lower eggshell quality in the late laying period leads to economic loss. It is a major threat to the quality and safety of egg products. Age-related variations in ultrastructure were thought to induce this deterioration. Eggshell formation is a highly complex process under precise regulation of genes and biological pathways in uterus of laying hens. Herein, we evaluated the physical, mechanical and ultrastructure properties of eggshell and conducted RNA sequencing to learn the transcriptomic differences in uterus between laying hens in the peak (young hens) and late phase (aged hens) of production. Results The declined breaking strength and fracture toughness of eggshell were observed in aged hen group compared to those in young hen group, accompanied with ultrastructure variations including the increased thickness of mammillary layer and the decreased incidence of early fusion. During the initial stage of eggshell formation, a total of 183 differentially expressed genes (DEGs; 125 upregulated and 58 downregulated) were identified in uterus of laying hens in the late phase in relative to those at peak production. The DEGs annotated to Gene Ontology terms related to antigen processing and presentation were downregulated in aged hens compared to young hens. The contents of proinflammatory cytokine IL-1β in uterus were higher in aged hens relative to those in young hens. Besides, the genes of some matrix proteins potentially involved in eggshell mineralization, such as ovalbumin, versican and glypican 3, were also differentially expressed between two groups. Conclusions Altered gene expression of matrix proteins along with the compromised immune function in uterus of laying hens in the late phase of production may conduce to age-related impairments of eggshell ultrastructure and mechanical properties. The current study enhances our understanding of the age-related deteriorations in eggshell ultrastructure and provides potential targets for improvement of eggshell quality in the late laying period.

show abstract

“…Hence, deteriorations in eggshell ultrastructure and quality in the late phase of production were speculated to be associated with age-related dysregulation of gene expression in uterus. It was supported by the ndings that the expression of genes encoding ion transporters and matrix proteins (ATP2A2, SCNN1G, CA2 and ovocalyxin-36) changed with the age of laying hens [19]. Age-related changes in the expression of matrix proteins (ovalbumin, ovotransferrin and ovocleidin-17) in uterus of laying hens were suggested by their varying concentration in eggshell [20,21].…”

Section: Introductionmentioning

confidence: 98%

Uterine Transcriptome Analysis Reveals mRNA Expression Changes Associated with the Ultrastructure Differences of Eggshell in Young and Aged Laying Hens

Feng

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Lower eggshell quality in the late laying period leads to economic loss. It is a major threat to the quality and safety of egg products. Age-related variations in ultrastructure were thought to induce this deterioration. Eggshell formation is a highly complex process under precise regulation of genes and biological pathways in uterus of laying hens. Herein, we evaluated the physical, mechanical and ultrastructure properties of eggshell and conducted RNA sequencing to learn the transcriptomic differences in uterus between laying hens in the peak (young hens) and late phase (aged hens) of production.Results: The declined breaking strength and fracture toughness of eggshell were observed in aged hen group compared to those in young hen group, accompanied with ultrastructure variations including the increased thickness of mammillary layer and the decreased incidence of early fusion. During the initial stage of eggshell formation, a total of 183 differentially expressed genes (DEGs; 125 upregulated and 58 downregulated) were identified in uterus of laying hens in the late phase in relative to those at peak production. The DEGs annotated to Gene Ontology terms related to antigen processing and presentation were downregulated in aged hens compared to young hens. The contents of proinflammatory cytokine IL-1β in uterus were higher in aged hens relative to those in young hens. Besides, the genes of some matrix proteins potentially involved in eggshell mineralization, such as ovalbumin, versican and glypican 3, were also differentially expressed between two groups.Conclusions: Altered gene expression of matrix proteins along with the compromised immune function in uterus of laying hens in the late phase of production may conduce to age-related impairments of eggshell ultrastructure and mechanical properties. The current study enhances our understanding of the age-related deteriorations in eggshell ultrastructure and provides potential targets for improvement of eggshell quality in the late laying period.

show abstract

Histological Change of Uterus Endometrium and Expression of the Eggshell-related Genes according to Hen Age

Cited by 8 publications

References 26 publications

The Influence of Hen Aging on Eggshell Ultrastructure and Shell Mineral Components

The Influence of Hen Aging on Eggshell Ultrastructure and Shell Mineral Components

Uterine transcriptome analysis reveals mRNA expression changes associated with the ultrastructure differences of eggshell in young and aged laying hens

Uterine Transcriptome Analysis Reveals mRNA Expression Changes Associated with the Ultrastructure Differences of Eggshell in Young and Aged Laying Hens

Contact Info

Product

Resources

About