Histological changes evoked in mice by Freund's complete adjuvant

Cellular Reaction in the Footpad and Draining Lymph Nodes of Mice Induced by Mycobacterial Fractions and BCG Bacilli

“…In the regional lymph nodes, lymphoid hyperplasia was present throughout the experimental period, and accumulations of macrophages appeared after 15 days, but granulomas were absent. These findings confirm the results of Carter et al (8), who found increased numbers of macrophages in regional lymph nodes after subcutaneous injection of Freund's adjuvant into the footpad but no granulomas. In the present study, some lymph nodes showed round nodular groups of macrophages, resembling granulomas, but, since such foci consisted only of macrophages and epitheloid cells were absent, they were not classified by us as granulomas.…”

Effect of Immunosuppression on the Development of the Adjuvant-Induced Granuloma in Mice

“…The tissue reaction of intact mice to injections of CFA were similar to those described previously [1,2,7], Although no quantitative evaluation of histologic changes was attempted there appeared to exist an obvious relationship between the number of small lymphocytes in the primary granuloma and the positive tuberculin skin test. Mice with a predominant granulocyte reaction at the site of the primary adjuvant injection showed less frequent lymphohistiocytic perivascular infiltrates when skin tested.…”

“…While thymectomy and antilympho cyte serum affected most markedly the cellular immune response and its morphologic expression in the lymph nodes, azathioprine was less specific acting on all cells from a certain state of differention onwards. The dissemination of the primary granuloma to other organs was not significantly influenced by immunosuppression.The histology of the local granulomatous inflammation caused by the injection of complete Freund's adjuvant (CFA) has been described pre viously [1,2], This reaction is accompanied by the development of a state of hypersensitivity representing a cellular immune response [3,4], It is assumed that pattern and extent of this histological reaction is related to the dissemination of Mycobacteria [2], The reactivation of remote tuber culous foci under immunosuppressive regimen is known [5] and may lead to subsequent dissemination of active disease.In this study the effect of immunosuppressive treatment on the develop ment of the tubercle granuloma was investigated experimentally, and the histologic changes were correlated with the presence or absence of …”

The migration of cells through chronically inflamed tissues

“…The histological changes that follow the injection of complete or incomplete Freund's adjuvant have been described by Moore et al (1963), Spector and Lykke (1966), and Carter, Jamison and Vollum (1968). There is a sequence of cellular events in the tissues which initially involves polymorphonuclear neutrophils, and subsequently monocytes, lymphocytes and plasma cells.…”