Histological changes of vascular clipping in Wistar rats

Abstract

Background: During aneurysm microsurgery, the aneurysmal sac is excluded from circulation by placing one or more clips at the base of the aneurysm. In some cases of complex aneurysms or subarachnoid hemorrhage history, transient clipping before definitive clipping is necessary. The closing force of the transient clip is less than the permanent clip; however, it is sufficient to stop circulation to the aneurysmal sac. The aim of the following work is to analyze and describe histological changes caused by transient and permanent clipping of the abdominal aorta in Wistar-type rats, to study the correlation between the closing force of the clip and the time, it remains on the vascular tissue structures. Methods: Six groups were formed, with 10 rats each, whereby temporary clipping of the abdominal aorta was performed with subsequent sampling of the site where the vascular clip was placed. The groups were: control and temporary clipping with: 2, 5, 10, and 15 and permanent clipping with 5 min. Results: Resection samples of the 3 μm thick aorta were obtained through the routine histological technique and special histochemical techniques (Masson’s Trichrome and orcein) from the six groups. Transmural changes were found from Group II–VI. Conclusion: There is a vascular histological effect after both transient and permanent clipping. The sum of time and strength of the clip induce vascular changes visible at 5 min.

Aerobic Exercise versus Electronic Cigarette in Vascular Aging Process: First Histological Insight

“…According to the CD68 marker immunohistochemistry results, the protein expressed in monocytes showed no macrophages, which means there had not been an early stage of atherosclerotic lesions [18]. This study showed that one of the earliest defects in e-cigarette seen from histological observations is the elastic fragmentation of the lamina [18][19][20]. Therefore, the absence of CD68positive staining is in fact supporting the evidence that electronic cigarette could have already caused elastic lamina fragmentation even before the early stage of atherosclerotic developed.…”

“…The study assessed that someone who smokes and is physically inactive has stiffer blood vessels than those who do not smoke and are physically inactive [ 26 ]. Smoking harms the vascular system, but there was no difference in the vascular stiffness in the active sports group, both smokers and nonsmokers [ 19 , 20 , 26 ]. The difference in our study with the Wistar rat experiment was directly observing the structure that contributes to the stiffness of blood vessels, namely, the elastic lamina.…”