Histological characterization of resistance in lactuca saligna to lettuce downy mildew (Bremia lactucae)

Lebeda, A.; Reinink, K.

doi:10.1016/s0885-5765(05)80106-7

Cited by 34 publications

(56 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two days after the treatment, leaf discs 12 mm in diameter were cut from treated leaves using a cork borer and inoculated with O. neolycopersici. At 48 h post-inoculation, five leaf discs were transferred to glacial acetic acid for 48 h and then mounted in glycerol prior to observations under a light microscope (Olympus BX50) (Lebeda and Reinink, 1994). Pathogen structures were stained with 1 % Evans Blue.…”

Section: Oidium Neolycopersici Phytoprotection Testmentioning

confidence: 99%

Diverse responses of wild and cultivated tomato to BABA, oligandrin andOidium neolycopersiciinfection

Satková

Starý

Plešková

et al. 2016

Ann Bot

View full text Add to dashboard Cite

Background and Aims Current strategies for increased crop protection of susceptible tomato plants against pathogen infections include treatment with synthetic chemicals, application of natural pathogen-derived compounds or transfer of resistance genes from wild tomato species within breeding programmes. In this study, a series of 45 genes potentially involved in defence mechanisms was retrieved from the genome sequence of inbred reference tomato cultivar Solanum lycopersicum 'Heinz 1706'. The aim of the study was to analyse expression of these selected genes in wild and cultivated tomato plants contrasting in resistance to the biotrophic pathogen Oidium neolycopersici, the causative agent of powdery mildew. Plants were treated either solely with potential resistance inducers or by inducers together with the pathogen.Methods The resistance against O. neolycopersici infection as well as RT-PCR-based analysis of gene expression in response to the oomycete elicitor oligandrin and chemical agent b-aminobutyric acid (BABA) were investigated in the highly susceptible domesticated inbred genotype Solanum lycopersicum 'Amateur' and resistant wild genotype Solanum habrochaites.Key Results Differences in basal expression levels of defensins, germins, b-1,3-glucanases, heveins, chitinases, osmotins and PR1 proteins in non-infected and non-elicited plants were observed between the highly resistant and susceptible genotypes. Moreover, these defence genes showed an extensive up-regulation following O. neolycopersici infection in both genotypes. Application of BABA and elicitin induced expression of multiple defence-related transcripts and, through different mechanisms, enhanced resistance against powdery mildew in the susceptible tomato genotype.Conclusions The results indicate that non-specific resistance in the resistant genotype S. habrochaites resulted from high basal levels of transcripts with proven roles in defence processes. In the susceptible genotype S. lycopersicum 'Amateur', oligandrin-and BABA-induced resistance involved different signalling pathways, with BABAtreated leaves displaying direct activation of the ethylene-dependent signalling pathway, in contrast to previously reported jasmonic acid-mediated signalling for elicitins.

show abstract

Section: Oidium Neolycopersici Phytoprotection Testmentioning

confidence: 99%

Diverse responses of wild and cultivated tomato to BABA, oligandrin andOidium neolycopersiciinfection

Satková

Starý

Plešková

et al. 2016

Ann Bot

View full text Add to dashboard Cite

show abstract

“…The materials were prepared as described below. 48 h glacial acetic acid (acetic acid 99%; Lach-Ner, Neratovice, Czech Republic), mounting, and storage in glycerol (glycerolum 85%; Tamda, Olomouc, Czech Republic), staining with 1% cotton blue (methyl blue, Anilin blue; Sigma-Aldrich, Prague, Czech Republic) before observation (Lebeda & Reinink 1994;Mieslerová et al 2004). The 4 th leaf of the tested plants was photographed.…”

Section: Methodsmentioning

confidence: 99%

“…At various terms (1/3, 1, 2, and 3 dai) the randomly selected leaf discs (12 mm in diameter) were cut from infected 4 th leaves (HS-treated and non-treated), then immersed for 48 h in glacial acetic acid, mounted in glycerol, and stained with 1% cotton blue prior to observation in an Olympus BX60 light microscope (Lebeda & Reinink 1994;Mieslerová et al 2004). Fungal development was assessed as the number of germ tubes per conidia, expressed in percent (%) of conidia producing germ tubes per 100 conidia, counted for each leaf disc at 1/3, 1, 2, and 3 dai; and as the length of the germ tubes assessed at 1/3, 1, 2, and 3 dai (1 st tube with lobbed appressorium, and 2 nd and 3 rd tubes with nipple shaped appressoria).…”

Section: Methodsmentioning

confidence: 99%

Effect of heat-shock pre-treatment on tomato plants infected by powdery mildew fungus

Nožková¹,

Mieslerová²,

Luhová³

et al. 2019

Plant Prot. Sci.

View full text Add to dashboard Cite

Citation: Nožková V., Mieslerová B., Luhová L., Piterková J., Novák O., Špundová M., Lebeda A. (2019): Effect of heat-shock pre-treatment on tomato plants infected by powdery mildew fungus. Plant Protect. Sci., 55: 31-42. Abstract:The effect of plant heat-shock (HS) pre-treatment (40.5°C, 2 h) on Pseudoidium neolycopersici development in the susceptible and moderately resistant Solanum spp. genotypes was studied together with biochemical responses (endogenous concentrations of salicylic (SA), jasmonic (JA), abscisic acid (ABA), and peroxidase (POX) activity). In HS pre-treated S. lycopersicum, an acceleration of pathogen, chlorosis and necrosis development, strong SA, JA accumulation, and increased POX activity were detected. In S. chmielewskii, HS pre-treatment caused a slight suppression of pathogen development, increase in JA, ABA concentrations, and POX activity. HS accelerated and strengthened the development of symptoms and biochemical responses to the infection in the susceptible genotype in contrast to moderately resistant genotype with a robust defence response to an infection per se.

show abstract

“…4). Infected leaf areas expand with time and ultimately turn brown (Sargent et al 1973;Lebeda and Reinink 1994;Lebeda et al 2008).…”

Section: Downy Mildewmentioning

confidence: 99%

“…Microscopic observations on L. saligna inoculated with B. lactucae indicated that NHR is mainly based on prehyphal or prehaustorial resistance. Only malformed hypha-like structures and no haustoria were observed (Lebeda and Reinink 1994;Zhang et al 2009b).…”

Section: Downy Mildew Controlmentioning

confidence: 99%

Nonhost wild lettuce as a donor for resistance to downy mildew in cultivated lettuce

Giesbers¹

View full text Add to dashboard Cite

Histological characterization of resistance in lactuca saligna to lettuce downy mildew (Bremia lactucae)

Cited by 34 publications

References 33 publications

Diverse responses of wild and cultivated tomato to BABA, oligandrin andOidium neolycopersiciinfection

Diverse responses of wild and cultivated tomato to BABA, oligandrin andOidium neolycopersiciinfection

Effect of heat-shock pre-treatment on tomato plants infected by powdery mildew fungus

Nonhost wild lettuce as a donor for resistance to downy mildew in cultivated lettuce

Contact Info

Product

Resources

About