Histological Demonstration of Bone Healing in Rat Tibiae Influenced by Diode Laser Irradiation

Yamazaki, Takahide; Kikui, Tetsuya; Yokose, Satoshi

doi:10.2530/jslsm.jslsm-37_0011

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…A bone defect-healing model in calvaria was adapted to the mental foramen bone to assess the effects of PRF on bone regeneration (1,28–31). Briefly, following anesthetisation with 4% sevoflurane, both sides of the mental foramen bone were treated with 100 µg of lidocaine and each side was surgically drilled to make holes 2.0 mm in diameter while avoiding injury to the roots of the teeth.…”

Section: Methodsmentioning

confidence: 99%

Platelet‑rich fibrin increases the osteoprotegerin/receptor activator of nuclear factor‑κB ligand ratio in osteoblasts

et al. 2019

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Platelet-rich fibrin (PRF) is a platelet concentrate derived from complete autologous blood rich in growth factors in the fibrin matrix. Although PRF has been used during oral surgery to optimize wound healing in soft and hard tissue, the precise role of PRF in bone healing remains unclear. The present study assessed the role of PRF in bone remodeling. PRF was prepared from whole blood by low speed centrifugation without any anti-coagulants. Culture of MC3T3-E1 cells with PRF induced the expression of osteoprotegerin (OPG), but had no effect on the expression of receptor activator of nuclear factor-κB ligand (RANKL), increasing the OPG/RANKL ratio. Expression of other osteoblastic differentiation makers, including BMP-2 and −4 and RUNX2, was not affected. PRF filling of a hole defect in the mental foramen bone of rats increased OPG positivity and decreased tartrate-resistant acid phosphatase positivity compared with unfilled control. In conclusion, PRF increased the OPG/RANKL ratio by inducing OPG expression, suggesting that PRF enhances early stage osteogenesis by optimizing osteoblastic differentiation. The present study provides a scientific basis for clinical findings showing that PRF can enhance bone regeneration such as sinus lift.

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Section: Methodsmentioning

confidence: 99%

Platelet‑rich fibrin increases the osteoprotegerin/receptor activator of nuclear factor‑κB ligand ratio in osteoblasts

et al. 2019

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“…In an in vivo study, Noda et al (2016) reported that laser irradiation significantly increased osteocalcin mRNA expression and proliferating cell nuclear antigen‐positive cell numbers in the wound healing of the tooth extraction socket model. Additionally, diode laser irradiation induced marked bone formation in the early phase of the bone healing process in a bone defect model using rat tibiae (Yamazaki et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…A high‐frequency pulsed diode laser (Lumix 2™, Fisioline s.r.l., Verduno, Italy) was employed, which emits pulse waves at a main wavelength of 904–910 nm with a peak power of 45 W, a maximum pulse repetition rate of 50 kHz, a pulse duration of 200 ns, and the diameter of the fibre is 1 mm. The laser was irradiated at 200 mW and 30 kHz for 7.5 min without any additional equipment from a vertical distance of 1 mm (2.0 mm spot diameter) on the ligated site at 1, 3, and 5 days after ligation, since 7.5 min of laser irradiation had induced marked bone formation in the early phase of the bone healing process in a bone defect model using rat tibiae (Yamazaki et al, 2016) and we had found that bone resorption was suppressed around 0.05 mm 3 by three times of 7.5 min of laser irradiation in ligature‐induced periodontitis in a pilot study. The total energy per day was 0.25 W and the total energy density per day was 114.6 J/cm 2 .…”

Section: Methodsmentioning

confidence: 99%

High‐frequency pulsed diode laser irradiation inhibits bone resorption in mice with ligature‐induced periodontitis

Ohsugi

Hatasa

Katagiri

et al. 2022

J Clinic Periodontology

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Aim The purpose of this study was to elucidate the suppressive effect of high‐frequency pulsed diode laser irradiation on bone resorption and its biological effects on gene expression and microbiome composition on the gingival tissue in ligature‐induced periodontitis in mice. Materials and Methods Ligating ligature around the teeth and/or laser irradiation was performed on the gingival tissue in mice as follows: Co (no ligature and no laser irradiation), Li (ligation without laser irradiation), La (no ligature but with laser irradiation), and LiLa (ligation with laser irradiation). Bone resorption was evaluated using micro‐computed tomography. RNA‐seq analysis was performed on gingival tissues of all four groups at 3 days after ligation. The differences in microbial composition between Li and LiLa were evaluated based on the number of 16S rRNA gene sequences. Results Bone resorption caused by ligation was significantly suppressed by laser irradiation. RNA‐seq in Co and La gingival tissue revealed many differentially expressed genes, suggesting diode laser irradiation altered gene expression. Gene set enrichment analysis revealed mTORC1 signalling and E2F target gene sets were enriched in gingival tissues both in La and LiLa compared with that in Co and Li, respectively. The amount of extracted DNA from ligatures was reduced by laser irradiation, and bacterial network structure was altered between the Li and LiLa. Conclusions High‐frequency pulsed diode laser irradiation showed biological effects and suppressed bone resorption in ligature‐induced periodontitis.

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Energy Medicine

Marquina¹

2017

The Scientific Basis of Integrative Health

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Histological Demonstration of Bone Healing in Rat Tibiae Influenced by Diode Laser Irradiation

Cited by 4 publications

References 25 publications

Platelet‑rich fibrin increases the osteoprotegerin/receptor activator of nuclear factor‑κB ligand ratio in osteoblasts

Platelet‑rich fibrin increases the osteoprotegerin/receptor activator of nuclear factor‑κB ligand ratio in osteoblasts

High‐frequency pulsed diode laser irradiation inhibits bone resorption in mice with ligature‐induced periodontitis

Energy Medicine

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