Histological Demonstration of Mucosal Invasion by Giardia lamblia in Man

Brandborg, Lloyd L.; Tankersley, Charlette B.; Gottlieb, Stephen E.; Barancik, Maurice; Sartor, Victor E.

doi:10.1016/s0016-5085(67)80001-5

Cited by 156 publications

(31 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Disturbance of intestinal motility, luminal competition by the parasite for substrates (Ament, 1972), bacterial colonisation (Yardley and Bayless, 1967;Ament and Rubin, 1972;Tandon et al, 1974) and epithelial damage produced by the parasite (Morecki and Parker, 1967) have been suggested as contributory factors. The role of tissue invasion in producing malabsorption is difficult to assess for the reports of its occurrence are very few (Morecki and Parker, 1967;Brandborg et al, 1967;Brandborg, 1971). Circulating antibody to G. lamblia in subjects with malabsorption (Ridley and Ridley, 1976) may indicate either that mucosal invasion is much more common than we at present appreciate or that this is only a marker of increased mucosal permeability with resultant absorption of parasite antigen.…”

Section: Discussionmentioning

confidence: 94%

Giardiasis: clinical and therapeutic aspects.

Wright¹,

Tomkins²,

Ridley³

1977

Gut

122

View full text Add to dashboard Cite

SUMMARY Malabsorption was present in 29 of 40 symptomatic patients with giardiasis. Twentythree had impaired D-xylose absorption; in 20 vitamin B12 absorption was low, and 15 patients had steatorrhoea. More severe malabsorption was associated with more marked histological abnormalities. Metronidazole, 2-0 g as a single daily dose on three successive days, produced a parasitological cure rate of 91%. In contrast, the standard course of mepacrine, 100 mg thrice daily for 10 days, eradicated the parasite in only 63% of patients. Improvements in absorption and jejunal morphology followed anti-giardial treatment. Tetracycline in eight patients failed to eradicate the parasite, intestinal absorption was unaltered, and histological appearances of the jejunal mucosa often deteriorated.

show abstract

Section: Discussionmentioning

confidence: 94%

Giardiasis: clinical and therapeutic aspects.

Wright¹,

Tomkins²,

Ridley³

1977

Gut

122

View full text Add to dashboard Cite

show abstract

“…Both humoral and T-cell immunity appear to be important. Penetration of Giardia trophozoites through breaks in the mucosa (32) or by actual inva sion (40) to intestinal lymphoid tissue may be important in the development of an immune response. Mice infected with large inocula of G. muris had a more rapid onset of infection, were sicker, and cleared the infection more rapidly than those receiving small inocula (41).…”

Section: (22)mentioning

confidence: 99%

Giardiasis

Smith¹,

Wolfe²

1980

Annu. Rev. Med.

View full text Add to dashboard Cite

Further ANNUAL REVIEWS PATHOGENESISEpidemiologic and experimental data suggest that individuals and various strains of experimental animals (21) differ in their susceptibility to infection, and that strains of G. lamblia differ in virulence. Immunocompromised individuals are partiCUla rl y likely to develop serious long-lasting infection Annu. Rev. Med. 1980.31:373-383. Downloaded from www.annualreviews.org Access provided by University of Waterloo on 02/05/15. For personal use only.

show abstract

“…Many species of protozoa induce diarrhea. In fact, protozoa such as Giardia lamblia, Toxoplasma gondii, Cryptosporidium parvum, and Entamoeba histolytica can invade and reproduce in the intestine, which is a cause of infection-associated diarrhea (Brandborg et al, 1967;Chen et al, 2000;Dubey et al, 1997;Saha et al, 1977;Yoshida et al, 2011). Since some myxosporean parasites invade and reproduce in the intestinal cells of the worm (El Matbouli et al, 1998), it has been hypothesized that Kudoa spp.…”

Section: Introductionmentioning

confidence: 99%

Kudoa septempunctataInvasion Increases the Permeability of Human Intestinal Epithelial Monolayer

Ohnishi

Kikuchi

Furusawa

et al. 2013

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

Kudoa septempunctata is a myxosporean parasite of Paralichthys olivaceus (olive flounder) and causes a foodborne illness that affects more than 100 cases in Japan each year. We previously reported that the consumption of raw olive flounder meat containing a high concentration of K. septempunctata spores induces transient but severe diarrhea and emesis through an unknown mechanism. Here, we demonstrate that K. septempunctata sporoplasm plays an important role in mediating the toxicity of K. septempunctata. When K. septempunctata spores were inoculated in Caco-2 human intestinal cells, K. septempunctata sporoplasms were released from spores, and they invaded the cells. Electron microscopic observations revealed that the sporoplasm invasion severely damaged the Caco-2 cells. The inoculation of K. septempunctata spores eliminated the transepithelial electrical resistance (TER) across the cell monolayer. Inhibiting the invasion of the sporoplasms prevented the observed loss in cell layer integrity, as illustrated by the rapid elimination of the TER. These results suggest that the invasion by sporoplasms severely damaged individual intestinal cells, resulting in a loss of cell monolayer integrity.

show abstract

Histological Demonstration of Mucosal Invasion by Giardia lamblia in Man

Cited by 156 publications

References 7 publications

Giardiasis: clinical and therapeutic aspects.

Giardiasis: clinical and therapeutic aspects.

Giardiasis

Kudoa septempunctataInvasion Increases the Permeability of Human Intestinal Epithelial Monolayer

Contact Info

Product

Resources

About