Histological Disorders Related to the Focal Disappearance of the Epiphyseal Growth Plate in Rats Induced by High Dose of Vitamin A.

Soeta, Satoshi; Mori, Ryoichi; Kodaka, Tetsuo; Naito, Yoshihisa; Taniguchi, Kazuyuki

doi:10.1292/jvms.62.293

Cited by 8 publications

(7 citation statements)

References 16 publications

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“…In addition to reduction of the hypertrophic cell zone, tearing of hypertrophic cell with signs of degeneration were observed. These findings are similar with studies that clarified that hypervitaminosis A induced chondroclast cells to absorb the calcified cartilage matrix and result in the erosions of hypertrophic zone (27,28).Also, Firat.D, Kuntsal .L and Sirin. Y,2005, had reported that Retinoic acid induced chondrocytes degeneration of fetal Meckel's cartilage (29).…”

Section: Discussionsupporting

confidence: 89%

Effect of Hypervitaminosis a on Growth of Mandibular Condyle in Rats

halawa

Abdelmajeed

El-Adway

et al. 2023

Alexandria Dental Journal

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BACKGROUND: Vitamin A is present in our food such as meat, dairy products, and vegetables. A control balanced diet maintains our nutritional needs of vitamin A. Over consumption of products as well as supplementations with vitamin A increases the risk of hypervitaminosis A. Hypervitaminosis A has been associated with thinning of the long bones and reducing of bone formation OBJECTIVES: to investigate the effect of over as well as optimum doses of vitamin A on growth of mandibular condyle in Wister albino rats. MATERIALS AND METHODS: Eight healthy (2month old age) male Wister albino rats weighting (274 -375grams) were used in present study. The rats were randomly divided into 2 equal group, study and control group .In the control group; rats fed a standard diet containing an optimum dose of vitamin A (12 IU/g of each pellet). In the study group, rats fed a standard diet supplemented with overdose of vitaminA (1700 IU /g of each pellet). After 2months, all rats were euthanized. Blood samples were collected from all rats the day after administering the last dose of vitamin and the concentration of vitamin A in serum was determined for all samples. The condyles of each rat of all groups were dissected out and processed for histological study and histomorphometric analysis. RESULTS: In the study group, the histological examination revealed marked reduction in thickness of hypertrophic zones and increase in the width of chondroblastic zones of condylar cartilage. Bone trabeculae were thin with irregular outline. The subchondral bone showed marked bone resorption with numerous osteoclasts in Howship's lacunae as compared with the control group. CONCLUSIONS: findings from the present study have indicated that, hypervitaminosis A increases resorption of the bone and diminishes endochondral bone growth.

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Section: Discussionsupporting

confidence: 89%

Effect of Hypervitaminosis a on Growth of Mandibular Condyle in Rats

halawa

Abdelmajeed

El-Adway

et al. 2023

Alexandria Dental Journal

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“…On the 12 th day, it appeared that abnormal cartilage matrix had developed in the epiphyseal growth plate of rats administered vitamin A. On the 19 th day there was a reduction in columnar arrangement and one or two focal losses in the epiphyseal growth plate of all rats administered vitamin A. Additionally, the calcified cartilage matrix surfaces were covered with a fine layer of bone tissue [13].…”

Section: Discussionmentioning

confidence: 99%

“…Some previous publications have shown that high dose vitamin A use reduces the epiphyseal growth plate [ 11 – 13 ]. Some publications have shown that excess vitamin A increases the number and volume of osteoclasts, reducing the osteoid surface causing bone resorption [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

Biochemical and histopathologic assessment of effects of acitretin on epiphyseal growth plate in rats

Önder

Bilgili

Bulut

et al. 2020

pdia

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Introduction: Acitretin is a commonly used retinoid in dermatology. Although there are generally known side effects, the effects on the epiphyseal plaque and bone metabolism are not clear in the literature. Aim: To histopathologically investigate the effects on the epiphyseal plate and assess variations in bone metabolism caused by acitretin. Material and methods: Three groups were formed with 10 rats in each group. The 1 st group (n = 10, 5 male, 5 female) were administered 10 mg/kg/day oral acitretin solution and the 2 nd group (n = 10, 5 male, 5 female) were administered 3 mg/kg/day oral acitretin solution. The control group were given normal standard feed and water. Rats were sacrificed at the end of 4 weeks. The proximal tibias were excised and histopathologically and immunohistochemically assessed. Biochemical assessment was also carried out. Results: Staining with haematoxylin-eosin found reductions in the epiphyseal plate in the 1 st and 2 nd group compared to the control group, though this situation was not statistically significant. Immunohistochemical studies did not encounter Type II collagen in the epiphyseal bone, proliferative zone and hypertrophic zone in the control group, low dose acitretin solution group and high dose acitretin solution group. Type II collagen was not observed in osteoids and osteoblasts. Type I collagen was not observed in the hypertrophic zone and proliferative zone of any group. Conclusions: Our data show that though acitretin caused degeneration of the epiphyseal plate, it did not cause clear thinning and we identified no significant variations in bone metabolism markers.

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“…Lectin-histochemistry for griffonia simplicifolia lectin (GSL)-I Lectin-histochemistry was performed according to Soeta et al (2000). Briefly, after blocking of endogenous peroxidase activity, sections were treated with 1% bovine serum albumin in 0.01 M phosphate-buffered saline (PBS; pH 7.4).…”

Section: Animalsmentioning

confidence: 99%

Accumulation of type VI collagen in the primary osteon of the rat femur during postnatal development

et al. 2015

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In rodents, the long bone diaphysis is expanded by forming primary osteons at the periosteal surface of the cortical bone. This ossification process is thought to be regulated by the microenvironment in the periosteum. Type VI collagen (Col VI), a component of the extracellular matrix (ECM) in the periosteum, is involved in osteoblast differentiation at early stages. In several cell types, Col VI interacts with NG2 on the cytoplasmic membrane to promote cell proliferation, spreading and motility. However, the detailed functions of Col VI and NG2 in the ossification process in the periosteum are still under investigation. In this study, to clarify the relationship between localization of Col VI and formation of the primary osteon, we examined the distribution of Col VI and osteoblast lineages expressing NG2 in the periosteum of rat femoral diaphysis during postnatal growing periods by immunohistochemistry. Primary osteons enclosing the osteonal cavity were clearly identified in the cortical bone from 2 weeks old. The size of the osteonal cavities decreased from the outer to the inner region of the cortical bone. In addition, the osteonal cavities of newly formed primary osteons at the outermost region started to decrease in size after rats reached the age of 4 weeks. Immunohistochemistry revealed concentrated localization of Col VI in the ECM in the osteonal cavity. Col VI-immunoreactive areas were reduced and they disappeared as the osteonal cavities became smaller from the outer to the inner region. In the osteonal cavities of the outer cortical regions, Runx2-immunoreactive spindle-shaped cells and mature osteoblasts were detected in Col VI-immunoreactive areas. The numbers of Runx2-immunoreactive cells were significantly higher in the osteonal cavities than in the osteogenic layers from 2 to 4 weeks. Most of these Runx2-immunoreactive cells showed NG2-immunoreactivity. Furthermore, PCNA-immunoreactivity was detected in the Runx2-immunoreactive spindle cells in the osteonal cavities. These results indicate that Col VI provides a characteristic microenvironment in the osteonal cavity of the primary osteon, and that differentiation and proliferation of the osteoblast lineage occur in the Col VI-immunoreactive area. Interaction of Col VI and NG2 may be involved in the structural organization of the primary osteon by regulating osteoblast lineages.

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Histological Disorders Related to the Focal Disappearance of the Epiphyseal Growth Plate in Rats Induced by High Dose of Vitamin A.

Cited by 8 publications

References 16 publications

Effect of Hypervitaminosis a on Growth of Mandibular Condyle in Rats

Effect of Hypervitaminosis a on Growth of Mandibular Condyle in Rats

Biochemical and histopathologic assessment of effects of acitretin on epiphyseal growth plate in rats

Accumulation of type VI collagen in the primary osteon of the rat femur during postnatal development

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