Histological effects of chronic sodium fluoride toxicity on some reproductive organs of male and female adult albino rats

Abstract

The current study aimed to determine the pathological effects of chronic poisoning with sodium fluoride on some reproductive organs like ovary, and testis of adult's male and female albino rats. Thirty-six male and female adult's albino rats were divided into six groups. The first and second group of male and female rats are control groups was given tap drinking water, the third and fourth groups of male and female rats was given 150 ppm of NaF, the fifth and sixth group of male and female rats was given 300 ppm of NaF respectively for 90 days. The weights of male and female genital were recorded. Histological exam of control groups of female rats showed the typical histological structure of the ovary, while the NaF treated groups showed a decrease in growing follicles, in addition to thickening in tunica albuginea and deposition of eosinophilic material. In male control groups, the sections showed the typical histological structures of the testis, while the treated groups showed multinucleated spermatids in addition to the deposition of amorphous eosinophilic material in the interstitial tissue, coagulative necrosis, in addition to apoptotic and sloughed spermatogonia in the lumen of seminiferous tubule. These results indicate that sodium fluoride with 300 ppm has toxic effects on organ body weights and on the histology of the gonads of adult's male and female albino rats.

Histological and histochemical observations of the prostate gland at resting and stimulating status in adult local Dog (Canis familiaris)

“…The specimens were processed by routine histological method for paraffin technique and sectioned serially at 5-7 µm. The prepared sections were stained with the hematoxylin and eosin, Masson's trichrome, combined PAS and modified alcian blue stains, and aldehyde fuchsine stain (7)(8)(9)(10). The histological observations were examined by using light microscope type (Olympus, BHC, Japan) and pictured by digital camera Sony type with a resolution of 2 megapixels (Optical Zoom, Japan) that USB connection with the computer slides which were pictured directly from the computer at various adjustment powers 100x, 40x and 10x (7,(11)(12)(13).…”