Histological evaluations and inflammatory responses of different dental implant abutment materials: A human histology pilot study

Sampatanukul, Teeratida; Serichetaphongse, Pravej; Pimkhaokham, Atiphan

doi:10.1111/cid.12562

Cited by 21 publications

(27 citation statements)

References 23 publications

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“…However, the expression was observed only in the bottom part of the epithelium, towards the Pt-Au alloy, which indicates weaker adhesion to the epithelium to the Pt-Au alloy compared to the titanium and zirconia. This may lead to the result of soft/hard tissue apical shift, which has been reported previously [30,32].…”

Section: Discussionmentioning

confidence: 78%

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Soft Tissue Interface with Various Kinds of Implant Abutment Materials

Furuhashi

Ayukawa

Atsuta

et al. 2021

JCM

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Various materials, such as titanium, zirconia and platinum-gold (Pt-Au) alloy, have been utilized for dental implant trans-mucosal parts. However, biological understanding of soft tissue reaction toward these materials is limited. The aim of this study was to compare the response of cell lines and soft tissue to titanium, zirconia and Pt-Au substrata. The surface hydroxyl groups and protein adsorption capacities of the substrata were measured. Next, gingival epithelial-like cells (Sa3) and fibroblastic cells (NIH3T3) were cultured on the materials, and initial cell attachment was measured. Immuno-fluorescent staining of cell adhesion molecules and cytoskeletal proteins was also performed. In the rat model, experimental implants constructed from various materials were inserted into the maxillary tooth extraction socket and the soft tissue was examined histologically and immunohistochemically. No significant differences among the materials were observed regarding the amount of surface hydroxyl groups and protein adsorption capacity. Significantly fewer cells of Sa3 and NIH3T3 adhered to the Pt-Au alloy compared to the other materials. The expression of cell adhesion molecules and a well-developed cytoskeleton was observed, both Sa3 and NIH3T3 on each material. In an animal model, soft tissue with supracrestal tissue attachment was observed around each material. Laminin-5 immuno-reactivity was seen in epithelia on both titanium and zirconia, but only in the bottom of epithelia on Pt-Au alloy. In conclusion, both titanium and zirconia, but not Pt-Au alloy, displayed excellent cell adhesion properties.

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Section: Discussionmentioning

confidence: 78%

“…A previous study found that the tissues around the gold alloy abutment showed a more negative reaction than titanium and zirconia abutments. The Pt-Au alloy was demonstrated to have less attachment resistance and a higher inflammatory response compared with titanium and zirconia abutments [30].…”

Section: Discussionmentioning

confidence: 99%

Soft Tissue Interface with Various Kinds of Implant Abutment Materials

Furuhashi

Ayukawa

Atsuta

et al. 2021

JCM

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“…This study is designed to directly analyse, in a human model, different materials used as supragingival components in daily implant dentistry. Though a few clinical studies have attempted to test some of the materials used (mainly titanium and zirconia) [ 10 , 12 , 29 ], the main limitations of prior studies is that they focused on one or two materials and/or the study design was not a randomized clinical trial and/or the analyses performed did not allow for the direct observation of the interface between the abutment material and soft tissues (i.e. instead of histology, other surrogate outcomes have been used).…”

Section: Discussionmentioning

confidence: 99%

Peri-implant soft tissue integration in humans – influence of materials: A study protocol for a randomised controlled trial and a pilot study results

Borie

Lambert

Lecloux

et al. 2020

Contemporary Clinical Trials Communications

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Background Recently, there has been a growing interest in mucointegration as the formation of an early and long-standing soft tissue barrier seems essential for both the initial healing and long-term implant survival. Aim To develop an experimental method to characterize the mucointegration of different transgingival materials (titanium (Ti), polyetheretherketone (PEEK), polymethylmethacrylate (PMMA), zirconia (Zi), polymer infiltrated ceramic network (PICN), cobalt-chrome (Co–Cr), and lithium disilicate (LD)) in a human model. Methods The study is designed as a multi-part randomized controlled clinical trial. Ninety bone level Straumann implants will randomly receive an experimental, custom-made abutment to allow for the removal of the abutment together with the surrounding soft tissues using a punch biopsy device at 8 weeks of healing (10 per material). The specimens will be further processed for non‐decalcified histology, followed by histomorphometric analysis. The same protocol will be used for additional 90 implants-abutments, but during harvesting, soft tissues will be separated from the abutment and processed for immunohistochemistry in order to study tissue inflammation and vascularization, while the abutments will undergo SEM analysis. Additionally, in vitro analyses, including SEM and profilometry, will be performed in order to characterize surface topography of all experimental materials. Conclusion The limited number of pilot samples presented herein indicate that the use of custom-made abutments in humans is a reproducible method to study peri-implant soft tissue integration. This further intensifies the rationale to compare different abutment materials, used as transgingival components in daily practice, under the same conditions.

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“…A previous study showed that gold alloy abutment demonstrates a poor attachment to soft tissue compared with titanium and zirconia abutments. Moreover, inflammatory extent and inflammatory cells in the soft tissue, around the gold alloy abutment, were significantly higher than those around titanium and zirconia abutments 16 . However, the characteristics of inflammatory cells around different abutment types are not fully clarified.…”

Section: Introductionmentioning

confidence: 93%

Immunohistochemical assessment of the peri‐implant soft tissue around different abutment materials: A human study

Serichetaphongse

Chengprapakorn

Thongmeearkom

et al. 2020

Clin Implant Dent Rel Res

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Background Soft tissue reaction to dental implant abutments influences the sustainability of restoration. Several abutment materials, such as titanium and gold alloy, have been introduced for strength and esthetic solutions. Zirconia and titanium‐base abutments have also become popular in recent years due to high esthetic demands. Purpose To investigate the immune cell infiltration into the soft tissue in response to different abutment materials. Materials and Methods Twenty dental implants were placed in the posterior regions of 17 patients. Titanium, zirconia, gold alloy, and titanium‐base were randomly selected for each patient on the day of surgery. After 8 weeks of healing, the abutments were removed along with 1 mm of peri‐implant soft tissue. Immunohistochemical assessment was performed using labeled streptavidin‐biotin to identify T cells, B cells, macrophages, plasma cells, and microvascular infiltration. Results Gold alloy demonstrated an overall higher inflammatory cell infiltration and highest number of CD3+, CD20+, and CD 68+ cells (P value <.05). The number of plasma cell and new microvascular infiltrations among abutment materials was not significantly different. Conclusion Titanium, titanium‐base, and zirconia abutments showed comparable infiltration profiles; gold alloy abutments showed the highest B‐cell, T cell, and macrophage infiltration. None of the abutment materials caused clinical inflammation; hence, they can be effectively used.

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Histological evaluations and inflammatory responses of different dental implant abutment materials: A human histology pilot study

Abstract: The tissue around the gold alloy abutments resulted in worse attachment conditions compared with the titanium and zirconia abutments. Inflammation tended to be higher in the tissue around the gold alloy abutments than the titanium and zirconia abutments.

Cited by 21 publications

References 23 publications

Soft Tissue Interface with Various Kinds of Implant Abutment Materials

Soft Tissue Interface with Various Kinds of Implant Abutment Materials

Peri-implant soft tissue integration in humans – influence of materials: A study protocol for a randomised controlled trial and a pilot study results

Immunohistochemical assessment of the peri‐implant soft tissue around different abutment materials: A human study

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