Histological in vitro evaluation of the effects of Er:YAG laser on oral soft tissues

Romeo, Umberto; Libotte, Fabrizio; Palaia, Gaspare; Vecchio, Alessandro Del; Tenore, Gianluca; Visca, P.; Nammour, Samir; Polimeni, Antonella

doi:10.1007/s10103-011-0969-z

Cited by 40 publications

(32 citation statements)

References 15 publications

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“…Due to the important role of the biopsy, as the specimen taken is uniquely and thus not repeatable, even from a medical-legal point of view, this procedure needs to be performed within the full safety margins [14,15]. Since laser has been used in a versatile way as a useful device in the surgical field, even for biopsies, there have been several studies aimed to test its safety [10,18,20]; but there are several authors who, especially in the past, valued controversies in the use of these devices in the field of biopsy surgery [14,15,24]. The critical aspect is not the difficult diagnosis of the specimen, since the various laser devices are now able to guarantee a histological diagnosis, if used with the right parameters and by a skilled operator, but the legibility of the peri-incisional margins of the specimen.…”

Section: Discussionmentioning

confidence: 99%

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Histological Effects of an Innovative 445 Nm Blue Laser During Oral Soft Tissue Biopsy

Palaia

Pergolini

D’Alessandro

et al. 2020

IJERPH

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Continuously evolving laser devices can be used in various fields; they are an alternative to the traditional cold blade surgery to perform biopsies of oral soft tissues. The aspect focused on in this paper is the possibility to use the 445 nm diode laser (Eltech K-Laser srl, Treviso, Italy) in complete safety, by evaluating its thermal effects during microscopy. A histological evaluation of the alteration of the peri-incisional edges on 10 samples was realized. All excisional biopsies were related to clinically unsuspected lesions and performed by the same expert operator. The surgical procedure was performed with the same laser parameters and the same pathologist evaluated the thermal effect on the samples. An average value of the detected tissue alteration was calculated; the average damage of the epithelium was 650.93 μm, while in the connective tissue it was 468.07 μm. In all the cases a clear diagnosis was possible, and no clinical complications were observed; so, the 445 nm diode laser proved to be a device that can be safely used for biopsies of clinically unsuspicious lesions. Due to the small number of cases, this in vivo preliminary experience needs to be extended.

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Section: Discussionmentioning

confidence: 99%

“…Several studies have been made about the thermal effect on soft tissues of the oral cavity, both "ex vivo" and "in vivo", with CO 2 [6,16], diode [17], KTP [18], Nd:YAG [19] and Er:YAG [20] laser devices.…”

Section: Introductionmentioning

confidence: 99%

Histological Effects of an Innovative 445 Nm Blue Laser During Oral Soft Tissue Biopsy

Palaia

Pergolini

D’Alessandro

et al. 2020

IJERPH

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“…Another study carried out by Romeo et al [14] about the histological evaluation of Er:YAG laser effect on oral soft tissues showed that, using this device with intermediate power (80–100 mJ), the thermal damage was always under the millimeter involving only the epithelium layer. So, authors concluded that thermal damages was negligible and the readability of the perioincisional margins was always possible.…”

Section: Discussionmentioning

confidence: 99%

Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological Evaluation

Romeo

Russo

Palaia

et al. 2014

The Scientific World Journal

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Introduction. Oral biopsy aims to obtain clear and safe diagnosis; it can be performed by scalpel or laser. The controversy in this latter application is the thermal alteration due to tissue heating. The aim of this study is the histological evaluation of margins of “in vivo” biopsies collected by diode and KTP lasers. Material and Methods. 17 oral benign lesions biopsies were made by diode 808 nm (SOL, DenMatItalia, Italy) and KTP 532 nm (SmartLite, DEKA, Italy). Samples were observed at OM LEICA DM 2000; margin alterations were evaluated through Leica Application Suite 3.4. Results. Epithelial and connective damages were assessed for each pathology with an average of 0.245 mm and a standard deviation of ±0.162 mm in mucoceles, 0.382 mm ± 0.149 mm in fibromas, 0.336 mm ± 0.106 mm in hyperkeratosis, 0.473 mm ± 0.105 mm in squamous hyperplasia, 0.182 mm in giant cell granuloma, and 0.149 mm in melanotic macula. Discussion. The histologic aspect of lesions influenced the response to laser, whereas the greater inflammation and cellularity were linked with the higher thermal signs. Many artifacts were also associated to histologic procedures. Conclusion. Both tested lasers permitted sure histologic diagnosis. However, it is suggested to enlarge biopsies of about 0.5 mm, to avoid thermal alterations, especially in inflammatory lesions like oral lichen planus.

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“…Esta absorción produce interacciones fotoquímicas, fototérmicas y fotomecánicas en los tejidos. En las mucosas se ha demostrado que el láser no ablativo Er:YAG estimula la producción de proteínas de matriz y fibroblastos, generando contracción de las fibras de colágeno y cambios tróficos que se manifiestan en tejidos con mayor grosor, hidratación y tensión (24)(25)(26)(27)(28).…”

Section: Discussionunclassified

Láser erbium vaginal como tratamiento del síndrome genitourinario de la menopausia: resultados preliminares

Gambacciani

2015

Rev. chil. obstet. ginecol.

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RESUMENAntecedentes: El síndrome genitourinario de la menopausia (SGM) afecta hasta el 50% de las mujeres posmenopáusicas. Sus síntomas presentan un curso crónico y progresivo, pudiendo alterar significativamente la sexualidad y la calidad de vida. Objetivo: Evaluar efectividad a corto plazo y aceptabilidad del láser vaginal de Erbium en cristal de itrio-aluminio-granate (Er:YAG) como una terapia fototérmica no invasiva, no ablativa para el manejo del SGM. Método: Estudio longitudinal prospectivo, realizado en 28 mujeres posmenopáusicas con SGM. Se aplica láser vaginal Er:YAG en 3 sesiones cada 30 días. En todos los controles, se evalúa sequedad vaginal, dispareunia mediante escala visual analógica, y se registra el "vaginal health index score" (VHIS). Al final del tratamiento se evalúa la aceptabilidad del método. Resultados: Se observa mejoría significativa de los síntomas (sequedad vaginal y dispareunia; p<0,01) durante todo el periodo de estudio y del VHIS (8,2 ± 1,3 previo al tratamiento vs. 19,2 ± 0,4 posterior al tratamiento; p<0,01). El 93,4% de las pacientes calificó el procedimiento como excelente o bueno. Conclusión: Nuestro estudio piloto muestra que el tratamiento con láser Er:YAG en mujeres posmenopáusicas con síntomas de SGM es eficaz, factible y seguro en el corto plazo. Se requieren datos a más largo plazo y de mayor tamaño muestral para confirmar nuestros resultados.PALABRAS CLAVE: Menopausia, estrógenos, láser Erbium, atrofia vulvovaginal, síndrome genitourinario de la menopausia SUMMARY Background:The genitourinary syndrome of menopause (GSM) affects up to 50% of postmenopausal women. Symptoms are chronic and likely worsen over time. This complains may interfere with sexual function and quality of life. Objective: To evaluate the short-term effectiveness and acceptability of Erbium in yttrium aluminum-garnet crystal Laser (Er:YAG) as a non-invasive, non-ablative photothermal therapy for the treatment of GSM. Methods: Prospective longitudinal study performed in 28 postmenopausal women suffering from GSM. All participants were treated intravaginally with Er:YAG laser in 3 applications performed every 30 days. Symptoms (vaginal dryness and dyspareunia) were evaluated by a visual-analogic scale (VAS) and the vaginal health index score (VHIS). After therapy, the women were asked to evaluate the acceptability of the intervention. Results: Er:YAG laser treatment was effective to improve symptoms (vaginal dryness and dyspareunia; p<0.01) at all the study period, as well the VHIS (8.2 ± 1.3 at baseline vs. 19.2 ± 0.4 after treatment; p<0.01). Satisfaction with the procedure was reported as excellent or good by 93.4% of women. Conclusion: Our pilot study shows that the Er:YAG treatment in postmenopausal women with GSM is effective, feasible, and safe. Further larger, long-term and controlled studies are required to confirm this data.

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Histological in vitro evaluation of the effects of Er:YAG laser on oral soft tissues

Cited by 40 publications

References 15 publications

Histological Effects of an Innovative 445 Nm Blue Laser During Oral Soft Tissue Biopsy

Histological Effects of an Innovative 445 Nm Blue Laser During Oral Soft Tissue Biopsy

Biopsy of Different Oral Soft Tissues Lesions by KTP and Diode Laser: Histological Evaluation

Láser erbium vaginal como tratamiento del síndrome genitourinario de la menopausia: resultados preliminares

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