Histological patterns and intra-tumor heterogeneity as prognostication tools in high grade serous ovarian cancers

Azzalini, Eros; Barbazza, Renzo; Stanta, Giorgio; Giorda, Giorgio; Bortot, L.; Bartoletti, Michele; Puglisi, Fabio; Canzonieri, Vincenzo; Bonin, Serena

doi:10.1016/j.ygyno.2021.09.012

Cited by 10 publications

(9 citation statements)

References 33 publications

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“…Their tumors frequently had a classic phenotype (mostly with an infiltrative and papillary pattern), psammoma bodies, a low mitotic rate and HR proficiency. These factors have already been associated with a poor outcome and response to chemotherapy [ 17 ] and, accordingly, in our study, the “low/low “group also displayed the highest rate of resistance.…”

Section: Discussionsupporting

confidence: 69%

“…Pascale” of Naples. Samples from CRO have already been characterized in two previous studies [ 16 , 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…to determine the histo-morphological features according to Soslow and colleagues [ 18 ]. The assessment of the number of mitoses and lymphocytes, tumor growth patterns and SET/Classic groups was carried out as previously described [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…ab16780) for 36 min at room temperature and the signal was detected with I-View DAB detection system. The semi-quantitative analysis of the antibody staining was performed using the H-score method as described elsewhere [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…The hematoxylin and eosin-stained slides of 100 samples together with the respective paraffin blocks were retrieved from the archives of the National Cancer Institute of Aviano (CRO), while 21 samples from the National Cancer Institute "G. Pascale" of Naples. Samples from CRO have already been characterized in two previous studies [16,17].…”

Section: Cases Selectionmentioning

confidence: 99%

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AKT Isoforms Interplay in High-Grade Serous Ovarian Cancer Prognosis and Characterization

Azzalini

Tierno

Bartoletti

et al. 2022

Cancers

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High-grade serous ovarian cancer (HGSOC) is among the deadliest gynecological malignancies. The acquired resistance to platinum-based therapies and the intrinsic heterogeneity of the disease contribute to the low survival rate. To improve patients’ outcomes, new combinatorial approaches able to target different tumor vulnerabilities and enhance the efficacy of the current therapies are required. AKT inhibitors are promising antineoplastic agents able to act in synergy with PARP inhibitors, but the spectrum of patients who can benefit from this combination is unclear, since the role of the three different isoforms of AKT is still unknown. Here, we study the expression of AKT isoforms on a retrospective cohort of archive tissue by RT-droplet digital PCR (ddPCR) analyzing their association with the clinicopathological features of patients. Based on AKT1/AKT2 and AKT1/AKT3 ratios, we define four AKT classes which were related to patients’ survival, tumor morphology and BRCA1 expression. Moreover, our results show that high AKT3 expression levels were frequently associated with tumors having classic features, a low number of mitoses and the presence of psammoma bodies. Overall, our study obtains new insights on AKT isoforms and their associations with the clinicopathological features of HGSOC patients. These evidences could help to better define the subsets of patients who can benefit from AKT and PARP inhibitors therapy in future clinical trials.

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Section: Discussionsupporting

confidence: 69%

“…Pascale” of Naples. Samples from CRO have already been characterized in two previous studies [ 16 , 17 ].…”

Section: Methodsmentioning

confidence: 99%