Histological preparation of teeth and tooth growth

Williamson, Raymond

doi:10.7243/2053-5775-3-3

Cited by 4 publications

(6 citation statements)

References 28 publications

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“…However, apparently wider bands in enamel than dentine are in part the result of differences in formation rate. Strontium always fluoresces more strongly in enamel than in dentine in SXRF maps (Figures 6, 10) although other fluorochrome and tetracycline labels that chelate or bind with Ca in dental tissues present a different picture where fluorescence in enamel is weaker than in dentine (Ibsen & Urist, 1964;Baker, 1972;Pautke et al, 2007;Antonini & Luder, 2011;Williamson, 2015). These observations fit the suggestion that, unlike tetracycline and fluorochromes, Sr is not removed from enamel by maturational ameloblasts, at least not to the same extent.…”

Section: Strontium Overprinting During Enamel and Dentine Mineralisationsupporting

confidence: 59%

Synchrotron X-ray fluorescence imaging of strontium incorporated into the enamel and dentine of wild-shot orangutan canine teeth

Dean

Cabec

Malderen

et al. 2020

Archives of Oral Biology

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Objectives: Dental hard tissues contain trace elements of both dietary and environmental origin. One objective was to demonstrate that a longitudinal record of synchronous Sr incorporation into enamel and dentine can be retrieved from museum specimens of once-free-living endangered species. Further objectives were to quantify sudden fluctuations in Sr concentration and estimate the extent of Sr overprinting back into dentine and enamel formed prior to the time of Sr ingestion. Materials and Methods: Daily incremental markings were used to determine rates and times of tooth formation and synchrotron X-ray fluorescence of the same polished ground sections to image Sr distribution in a male and a female orangutan canine. The X-ray beam was monochromatised to 17.0 keV and focused to 500x500 nm 2 . Scans were performed at either 25.0 or 5.0 µm resolution. Results: Baseline Sr levels ranged between 215-750 ppm. Multiple short, intense Sr labels reaching 750-1,625 ppm occurred randomly throughout 15-22 years of tooth formation. In dentine, Sr concentration increased gradually away from the EDJ, while in enamel, it reduced towards the enamel surface. Using daily incremental markings, Sr overprinting into earlier formed dentine and enamel was estimated to be ~12-45 days. There was no evidence of Sr overprinting by maturational ameloblasts. Conclusions: A good record of growth and trace element incorporation into tooth tissues can be retrieved from museum specimens. Short, intense Sr labels were equally well time-resolved in enamel and dentine and could be distinguished from more diffuse background levels. Enamel maturation appears to have no quantifiable effect.

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Section: Strontium Overprinting During Enamel and Dentine Mineralisationsupporting

confidence: 59%

Synchrotron X-ray fluorescence imaging of strontium incorporated into the enamel and dentine of wild-shot orangutan canine teeth

Dean

Cabec

Malderen

et al. 2020

Archives of Oral Biology

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“…In Denmark each mink gets around 200 g feed per day, hence a female mink weighing 1.5kg gets around 90mg TC per kg bodyweight per treatment. A fraction of the ingested TC is subsequently embedded in the growth layers of the dentin, dental cements and bone tissue, where it is detectable by epifluorescence microscopy for several years or for the rest of the animal´s life [22,23]. TC appears as yellow florescent markings (Fig 2).…”

Section: Introductionmentioning

confidence: 99%

Methods for the identification of farm escapees in feral mink (Neovison vison) populations

et al. 2019

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In Denmark, American mink (Neovison vison) have been bred for their fur since the mid-1920s. Mink escaping from farms may supply the feral population. Often, it is of biological and management interest to separate the population of feral mink (i.e. mink caught in the wild) in two groups: 1) mink born on farms i.e., escapees, and 2) mink born in the wild. In this study, two methods were used for separating feral mink into the two groups: a) Comparison of body length of farmed mink and feral mink, and b) Presence of a biomarker (tetracycline: an oral antibiotic used on mink farms). A total of 367 wild caught mink (from the mainland of Denmark and the island of Bornholm), and 147 mink from farms, collected during the period 2014–2018, were used for the analysis of body length. For the testing of tetracycline (TC) as a biomarker, 78 mink from farms where there was knowledge about TC treatment (with or without) were examined for fluorescent markings in the canine teeth. Results from both univariate analyses and Gaussian mixture model analysis demonstrated clear divisions between the mean body length (mean ± S.E., range) of farmed males (52.1 cm ± 0.4, 48–68) and farmed females (mean 44.0 ± 0.2, 40–50), and between farmed mink and wild caught mink. Mixture analysis identified two groups within each sex of the wild caught mink, one assigned to farmed mink (born in captivity) and another group of smaller mink suspected of being born in the wild. On Bornholm, the mean (±SD, range) length of males born in the wild was 43.7cm (± 0.3, 36–57) and for females 37.5cm (± 0.3, 32–45). The mean length (±SD, range) of males born in the wild in the mainland of Denmark was 42.5cm (± 2.3, 36–46) and for females 36.1cm (± 1.0, 34–37). Among the feral mink from mainland Denmark, 28.4% of males and 21.6% of females were identified as escapees, while 0% of the males and 1% of the females were identified as escapees among the wild caught mink on Bornholm. Eight percent of mink from farms using tetracycline were false negatives, while no false positives were found among mink from farms not using TC. TC fluorescence was found in five of 217 mink caught in the wild equivalent to 22% escapees in mainland Denmark. No TC markings were found in mink caught in the wild on Bornholm. In conclusion, both methods a) the body length of mink, and b) fluorescent biomarkers in canine teeth are considered as useful tools to identifing mink that have escaped from farms.

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“…The most common methods are presented in Table 1. 8,[11][12][13][14][15][16][17][18][19][20][21][22][23] Dominici et al compared the effectiveness of 5 different methods of sterilization/disinfection of extracted human teeth. 11 Antimicrobial eradication was determined by the absence of Bacillus stearothermophilus (heat-resistant bacteria used to validate sterilization).…”

Section: Sterilization Of Extracted Human Teethmentioning

confidence: 99%

“…7 Vertical, horizontal and cross-sections are investigated with microscopic techniques in histological studies. 8 The crystalline structure of the teeth is revealed by observing non-decalcified sections under polarized light, and then the sample is decalcified to analyze the soft tissue component. Samples used in immunohistochemistry studies must be precisely prepared; sections can be obtained through cryomicrotomy (a freezing microtome).…”

Section: Introductionmentioning

confidence: 99%

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Extracted human teeth and their utility in dental research. Recommendations on proper preservation: A literature review

Nawrocka¹,

Łukomska−Szymańska²

2019

Dent. Med. Probl.

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Laboratory research in dentistry and dental education use extracted human teeth as a model for simulation for ex vivo procedures. Human-borne tissues are the first choice of material for bond strength assessment. To obtain comparable results in dental material tests and to ensure microbiological safety, specimens must be stored under specific, uniform conditions. The aim of this paper was to present the contemporary view and recommendations on preserving extracted human teeth. The antimicrobial properties of the storage medium are a crucial aspect, as extracted teeth pose a risk of cross-infection. A classification of different methods (using solutions and otherwise) is presented and their sterilizing efficiency is compared based on the literature. The emphasis is put on the interaction between the storage conditions and the substrate. Tooth specimens should be biologically safe and have normal mechanical properties. The sterilizing process must be neutral for the enamel and dentin microstructure, because even a minor change can affect the adhesive bonding. Autoclave sterilization and storage in 10% formalin solution are widespread and reliable methods, although they do have their disadvantages. There is a need for further investigation in order to establish uniform recommendations on preparing and preserving extracted human teeth used for research purposes.

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Histological preparation of teeth and tooth growth

Cited by 4 publications

References 28 publications

Synchrotron X-ray fluorescence imaging of strontium incorporated into the enamel and dentine of wild-shot orangutan canine teeth

Synchrotron X-ray fluorescence imaging of strontium incorporated into the enamel and dentine of wild-shot orangutan canine teeth

Methods for the identification of farm escapees in feral mink (Neovison vison) populations

Extracted human teeth and their utility in dental research. Recommendations on proper preservation: A literature review

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