Histological studies of shoot regeneration system in hypocotyl-derived callus of Phellodendron amurense Rupr.

Abstract

An efficient in vitro plant regeneration was achieved from hypocotyl-derived callus of a medicinal tree Phellodendron amurense. The expected morphogenic response was obtained on the media containing 0.89 mM 6-benzyl aminopurine (BAP) plus 4.52 mM 2.4-dichlorophenoxyacetic acid (2,4-D), 4.44 mM BAP plus 5.37 mM a-naphthaleneacetic acid (NAA), or 2.22 mM BAP plus 4.92 mM indole-3-butyric acid (IBA). A detailed histological study was undertaken to gain a better understanding of cellular changes that take place during the plant regeneration process from callus tissue. This study led to the identification of the cellular origin of shoot regeneration. Histological studies revealed that the new vegetative buds originated from callus that completely altered the morphology of the callus tissues by the 60th day of culture. It also revealed that BAP with NAA or IBA induces plant regeneration through organogenesis whereas BAP with 2,4-D induces embryo-like-structure (ELS) through hypocotylderived callus. The presence of ELS lacking root poles was also observed.

Plant regeneration through somatic embryogenesis of a medicinal plant, Phellodendron amurense Rupr.

“…Therefore, we could say that 2,4-D produces SEs but blocks root induction. Azad et al (2005c) reported the similar observation in P. amurense.…”

“…(e) Germination of SE without rooting showing shoot apex (SA), leaf primordium (LP), and PC, which were developed in MS medium containing 8.8 μM BA plus 4.0 μM 2,4-D. f Germination of SE showing SA, LP, PC, and RP which were developed in MS medium containing 8.8 μM BA plus 4.0 μM NAA. NB: (e) cited byAzad et al (2005c).…”

Plant regeneration through somatic embryogenesis and synthetic seed production in Rumex vesicarius L. – A Potent medicinal herb

“…In our present investigation type II callus showed greenish yellow nodular structure as in case of Curculigo orchioides [23]. Different developmental stages of somatic embryo like globular, leaf primordials were evident from histological studies of leaf derived calli as in case of Phellodendron amurense [24] Alginate is one of the common polymer used for production of synthetic seeds. It exhibits greater variation in viscosity, resulting in difficulty for decanting and encapsulation.…”

Provenance variations in berberine content ofPhellodendron amurense, a rare and endangered medicinal plant grown in Northeast China

“…Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions Introduction Phellodendron amurense Rupr. (Rutaceae) is a deciduous tree commonly called the Amur cork tree (Azad, Yokota, Ishiguri, et al 2005;Wang et al 2009). Indigenous to the Amur Valley at the boundary between Northeastern China and the Russian Far East (Lis et al 2004), P. amurense is now distributed in Northeastern China, Inner Mongolia, the Russian Far East, and southern Sakhalin, Korea, and Japan (Yang et al 2013).…”