Histological study and immunohistochemical location of cytoskeletal proteins in the ovaries of the three species of lizards of the family Leiosauridae (Reptilia: Squamata)

Firmiano, Enely Maris da Silveira; Machado-Santos, Clarice; Serra-Campos, Amanda Oliveira; Sousa, Bernadete Maria de; Pinheiro, Nadja Lima; Nascimento, Aparecida Alves do

doi:10.1016/j.tice.2020.101477

Cited by 3 publications

(3 citation statements)

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“…Histological characterization in reproductive system has been described in lizards of Suborder Iguania, Family (a) Phrynosomatidae: in Sceloporus variabilis (Peña-Herrera et al, 2020); Sceloporus aeneus (Hernández-Gallegos et al, 2014); (b) Tropiduridae: Eurolophosaurus nanuzae (Melo et al, 2019); Tropidurus torquatus (Silva et al, 2016) and Tropidurus itambere (Ferreira et al, 2009). However, similar studies for the lizards Enyalius bilineatus, Enyalius perditus and Urostrophus vautieri, of the Leiosauridae family from the same suborder, have been described only in female lizard (da Silveira Firminiano et al, 2021).…”

Section: Introductionmentioning

confidence: 82%

“…Vimentin is expressed in all derived mesenchymal cells, such as peritubular myoid cells, fibroblasts and endothelial cells, which are all present in the testicular interstitium or tunica albuginea (Aire & Ozegbe, 2008; Bernardino et al, 2018; De Chiara et al, 2018; Rotgers et al, 2015; Zomer & Reddi, 2020). Intermediate filaments and microfilaments have already been studied through immunohistochemical studies in the ovary of Enyalius bilineatus, Enyalius perditus and Urostrophus vautieri (da Silveira Firminiano et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Histological study and immunohistochemical location of cytoskeletal proteins in the testis and epididymis of the three species of lizards of the family Leiosauridae (Reptilia: Squamata)

et al. 2022

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The objective of the study was to histologically describe the structure of the testis and epididymis, identifying the GAGs in them. Furthermore, the distribution of smooth muscle alpha‐actin, desmin, and vimentin is described of E. bilineatus, E. perditus, and U. vautieri, through immunohistochemical method. Two adult males of each species of the lizards were collected in southeastern Brazil. In the testicular albuginea, the presence of neutral glycoproteins was detected. In E. bilineatus, the epididymal duct epithelium is simple, composed of secretory cells, cylindrical in shape and acidophilic cytoplasm. In the testis of the three lizards there was a positive immunolocation accentuated for α‐SMA in the dense connective tissue of the tunica albuginea. A moderate reaction was observed in the connective tissue albuginous and in the layer of smooth muscle fibers in a circumferential arrangement of the epididymal duct. The immunolocation for desmin was accentuated in the fibers of the testicular albuginea of E. bilineatus, while in U. vautier and E. perditus was moderate. In the epididymis, only in E. perditus and E. bilineatus positive immunoreaction was verified. In the three lizards there were no observations of immunostaining for vimentin with the antibody utilized.

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Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Histological study and immunohistochemical location of cytoskeletal proteins in the testis and epididymis of the three species of lizards of the family Leiosauridae (Reptilia: Squamata)

et al. 2022

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“…A malignant Sertoli cell tumor was not completely ruled out given the presence of prominent connective septa, cytoplasmic vacuolation, and positive immunolabeling with NSE on the first round of immunohistochemical characterization—a marker expressed in Sertoli cell tumors in both mammalian and non-mammalian species ( 12 , 13 ). A Sertoli cell tumor, could, however, not be confirmed because immunolabeling with vimentin —a mesenchymal marker expressed in testicular tumors in mammals—was inconclusive (Meuten et al, 2016), as it was the case in normal testis of lizards of the Leiosauridae family ( 15 ). Second, antibodies specific to Sertoli cells (e.g., anti-Müllerian hormone and inhibin α) that have successfully been used in non-mammalian species were not available to the authors ( 13 , 16 ).…”

Section: Discussionmentioning

confidence: 99%

Case report: Intracoelomic neoplastic mass of undetermined origin in an Asia minor spur-thighed tortoise (Testudo graeca ibera)

François,

Paillusseau,

Bagatella

et al. 2023

Front. Vet. Sci.

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This article describes the diagnostic, treatment and attempted characterization of a neoplasia of undetermined origin in a Asia minor spur-thighed tortoise. A 21-year-old male Asia minor spur-thighed tortoise (Testudo graeca ibera) was admitted for a 4-month history of diarrhea, and a 2-month history of anorexia and lethargy. Physical examination revealed a firm midcoelomic mass in the right prefemoral fossa. Blood biochemistry indicated hypocalcemia and mild elevation of aspartate aminotransferase. Supportive care was administered in the form of heating, baths, and calcium injections. Ultrasound examination of the coelomic cavity revealed a 6-cm diameter, highly vascularized mass with liver-like echogenicity. Neoplasia was suspected, and endoscopy was performed, revealing a brown circumscribed mass with smooth edges. Surgical removal of the mass was evaluated by CT scan and achieved via a plastrotomy; however, the patient died 1 day post-surgery. The mass was located on the dorsal right side of the coelomic cavity in the anatomic location of the right testicle. Histopathology revealed neoplastic cells organized in packets supported by fibrous septa. Neoplastic cells showed moderate and inconsistent positive immunohistochemical labeling for S100 and NSE, and negative immunohistochemical labeling for pan-cytokeratin, vimentin, CD3, CD79a, chromogranin A, and synaptophysin. The prominent histological and anatomical characteristics of the mass indicated a possible testicular or neuroendocrine (e.g., adrenal gland) origin. Due to inconclusive immunohistochemical profiles and poorly differentiated neoplastic cells, only a final diagnosis of intracoelomic malignant tumor of undetermined origin could be established. This case underscores the difficulties encountered in achieving definitive diagnoses of neoplastic diseases in reptile medicine.

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Structural gonadal lesions observed in Japanese quail (Coturnix coturnix japonica) following exposure during puberty to the neonicotinoid pesticide, imidacloprid

Ibrahim,

Lensink,

Phaswane

et al. 2024

Tissue and Cell

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Histological study and immunohistochemical location of cytoskeletal proteins in the ovaries of the three species of lizards of the family Leiosauridae (Reptilia: Squamata)

Cited by 3 publications

References 34 publications

Histological study and immunohistochemical location of cytoskeletal proteins in the testis and epididymis of the three species of lizards of the family Leiosauridae (Reptilia: Squamata)

Histological study and immunohistochemical location of cytoskeletal proteins in the testis and epididymis of the three species of lizards of the family Leiosauridae (Reptilia: Squamata)

Case report: Intracoelomic neoplastic mass of undetermined origin in an Asia minor spur-thighed tortoise (Testudo graeca ibera)

Structural gonadal lesions observed in Japanese quail (Coturnix coturnix japonica) following exposure during puberty to the neonicotinoid pesticide, imidacloprid

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