Histological Study of Suprabranchial Chamber Membranes in Anabantoidei and Clariidae Fishes

Abstract

Accessory respiratory organs (AROs) are a group of anatomical structures found in fish, which support the gills and skin in the process of oxygen uptake. AROs are found in many fish taxa and differ significantly, but in the suborder Anabantoidei, which has a labyrinth organ (LO), and the family Clariidae, which has a dendritic organ (DO), these structures are found in the suprabranchial cavity (SBC). In this study, the SBC walls, AROs, and gills were studied in anabantoid (Betta splendens, Ctenopoma acutirostre, Helostoma temminckii) and clariid (Clarias angolensis, Clarias batrachus) fishes. The histological structure of the investigated organs was partially similar, especially in relation to their connective tissue core; however, there were noticeable differences in the epithelial layer. There were no significant species-specific differences in the structure of the AROs within the two taxa, but the SBC walls had diversified structures, depending on the observed location. The observed differences between species suggest that the remarkable physiological and morphological plasticity of the five investigated species can be associated with structural variety within their AROs. Furthermore, based on the observed histology of the SBC walls, it is reasonable to conclude that this structure participates in the process of gas exchange, not only in clariid fish but also in anabantoids.

Association of predation risk with a heterospecific vocalization by an anabantoid fish

“…Some non-otophysan fishes have independently become hearing specialists. The Anabantoidei (gouramis, bettas) of equatorial Asia and Africa occur in warm-water bodies with low levels of dissolved oxygen (Adamek-Urba nska et al, 2021). These fishes possess a labyrinth organ of vascularized canals in their head (the suprabranchial chamber) that hold atmospheric air for the purpose of gas exchange.…”

Experimental Studies on the Impact of the Projected Ocean Acidification on Fish Survival, Health, Growth, and Meat Quality; Black Sea Bream (Acanthopagrus schlegelii), Physiological and Histological Studies

“…Thin sample sections were stained with hematoxylin and eosin for histological description according to the standard method described by Munro [ 51 ] and used by Titford [ 52 ]. The gill, liver, skin, foregut midgut, and hindgut tissues were stained with H&E, put on small rectangle glass slides, and observed with the light microscope (Axiocam 506 color, ZEISS) [ 53 , 54 , 55 ]. All images were obtained in high-definition.…”

Anesthetic effect and acute toxicity of Citrus sinensis essential oil in betta

“…The slides were taken to an optical microscope for observation and photographed using the Leica Application Suite (LAS) software (40× magnification), then the height in microns (μm) of 10 gill filaments was measured. The height was obtained from the base to the apex of each filament, and the parameters evaluated according to the protocol previously described by Heluy et al (2020) and Adamek-Urbańska et al (2021).…”