Histological study of the effects of treatment with gonadotropin-releasing hormone agonist (GnRHa) on the reproductive maturation of captive-reared Atlantic bluefin tuna (Thunnus thynnus L.)

Corriero, Aldo; Medina, Antonio; Mylonas, Constantinos C.; Abascal, F.J.; Deflorio, M.; Aragón, Lourdes; Bridges, C.R.; Santamaría, N.; Heinisch, Gilad; Robert, Vincent; Belmonte, Antonio; Fauvel, Christian; Garcı́a, Antonio G.; Gordin, H.; Metrio, G. De

doi:10.1016/j.aquaculture.2007.08.030

Cited by 56 publications

(48 citation statements)

References 32 publications

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“…Indeed, considerable numbers of gonadal myoid cells were observed in the immature testes and ovaries of farmed bluefin tuna examined in this study. Similar histological observations with abundant myoid cells were also reported in bluefin tuna reared under different conditions [30][31][32]. Thus, in order to increase the rate of successful transplantation in tuna using SG, it is desirable to establish a spermatogonial enrichment procedure, such as magnetic or fluorescence-activated cell sorting procedures using cell surface antigens.…”

Section: Cloning Of Vasa Cdna and Phylogenetic Analysissupporting

confidence: 71%

cDNA cloning and expression analysis of a vasa-like gene in Pacific bluefin tuna Thunnus orientalis

et al. 2008

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In this study, a Pacific bluefin tuna (Thunnus orientalis) homolog of the Drosophila vasa gene, BtVLG (bluefin tuna vasa-like gene), was cloned and characterized for use as a molecular marker for germ cells in this species. Analysis of the nucleotide sequence revealed that BtVLG comprises 2,394 bps with an open reading frame of 1,932 bps encoding 644 amino acids. The deduced amino acid sequence contained arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The BtVLG sequence showed high similarity to Drosophila vasa (69.1%), zebrafish vasa homolog (80.5%), and tilapia vasa homolog (91.2%). In adult tissues, the BtVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that BtVLG messenger RNA (mRNA) was detected in oogonia and previtellogenic oocytes in the ovary. In the testis, while BtVLG mRNA was detected in spermatogonia, it was not detected in the primary and secondary spermatocytes, spermatids, spermatozoa or gonadal somatic cells. Consequently, consensus sequences, sequence similarity, and specific localization of BtVLG mRNA in the germ cells all suggest that BtVLG is the bluefin tuna vasa homolog of the Drosophila vasa gene. Further, BtVLG can be used as a molecular marker for bluefin tuna germ cells.

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Section: Cloning Of Vasa Cdna and Phylogenetic Analysissupporting

confidence: 71%

cDNA cloning and expression analysis of a vasa-like gene in Pacific bluefin tuna Thunnus orientalis

et al. 2008

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“…Importantly, the homology of FSHb and LHb to bluefin tuna was very high. Bluefin tuna is a commercially important cultured fish species, and reproductive problems such as the inhibition of FOM and a late age of sexual maturity is a serious issue (Corriero et al 2007(Corriero et al , 2009. It is difficult to analyze the physiological mechanism regulating gametogenesis in reared bluefin tuna because the adult body size is very large (30-150 kg) (Sawada et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Changes in the expression of pituitary gonadotropin subunits during reproductive cycle of multiple spawning female chub mackerel Scomber japonicus

Nyuji

Sethu

Kitano

et al. 2011

Fish Physiol Biochem

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The endocrine regulation of reproduction in a multiple spawning fish with an asynchronous-type ovary remains largely unknown. The objectives of this study were to monitor changes in the mRNA expression of three gonadotropin (GtH) subunits (GPα, FSHβ, and LHβ) during the reproductive cycle of the female chub mackerel Scomber japonicus. Cloning and subsequent sequence analysis revealed that the cDNAs of chub mackerel GPα, FSHβ, and LHβ were 658, 535, and 599 nucleotides in length and encoded 117, 115, and 147 amino acids, respectively. We applied a quantitative real-time PCR assay to quantify the mRNA expression levels of these GtH subunits. During the seasonal reproductive cycle, FSHβ mRNA levels remained high during the vitellogenic stages, while GPα and LHβ mRNA levels peaked at the end of vitellogenesis. The expression of all three GtH subunits decreased during the post-spawning period. These results suggest that follicle-stimulating hormone (FSH) is involved in vitellogenesis, while luteinizing hormone (LH) functions during final oocyte maturation (FOM). Both GPα and FSHβ mRNA levels remained high during the FOM stages of the spawning cycle and increased further just after spawning. Thus, FSH synthesis may be strongly activated just after spawning to accelerate vitellogenesis in preparation for the next spawning. Alternatively, LHβ mRNA levels declined during hydration and then increased after ovulation. This study demonstrates that chub mackerel are a good model for investigating GtH functions in multiple spawning fish.

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“…According to Takahashi (2006), the fecundity estimated for S. hilarii in the wild was 54,157 ± 19,991 oocytes (with diameters reaching no more than 1428 lm), which is similar to our data found for females collected in the river. Lower fecundity was also found in the Atlantic bluefin tuna (Thunnus thynnus) in captivity when compared to wild animals, and the authors suggest a probable dysfunction in vitellogenin plasma levels (Corriero et al 2007). However, Duponchelle et al (2000) observed that, under conditions of confinement, some asynchronous species (tilapias) show higher fecundity, but the oocytes produced are smaller when compared to animals collected in the natural environment.…”

Section: Mature/advanced Maturationmentioning

confidence: 92%

Patterns of oocyte development in natural habitat and captive Salminus hilarii Valenciennes, 1850 (Teleostei: Characidae)

Honji

Narcizo

Borella

et al. 2008

Fish Physiol Biochem

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Fecundity and oocyte development in Salminus hilarii female brood stock were analyzed with the aim of investigating the impact of migration impediment on oogenesis. Histological analyses of the ovaries were performed in adult females caught in two different environments--the Tietê River (natural) and captivity--and the gonadossomatic index, oocyte diameter and fecundity determined. Five germ cell development stages (oogonium, perinucleolar, cortical alveoli, vitellogenic, ripe) and two other structures (postovulatory follicles and atretic oocytes) were observed in females caught in the river. Captive animals lacked the ripe oocytes and postovulatory follicles and had a relatively higher number of atretic oocytes. Females in captivity are known to produce larger oocytes, and they release fewer eggs in each spawn (absolute fecundity) when compared with animals that are able to migrate. Our results suggest that the Tietê River is undergoing alterations which are being reflected in the reproductive performance of S. hilarii, mainly due to the presence of atretic oocytes in females caught in the river. The lack of postovulatory follicles and ripe oocytes in captive animals reveals that migratory impediment negatively impacts final oocyte maturation. However, the stage of maturation reached is adequate for ovulation induction with hormone manipulation.

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Histological study of the effects of treatment with gonadotropin-releasing hormone agonist (GnRHa) on the reproductive maturation of captive-reared Atlantic bluefin tuna (Thunnus thynnus L.)

Cited by 56 publications

References 32 publications

cDNA cloning and expression analysis of a vasa-like gene in Pacific bluefin tuna Thunnus orientalis

cDNA cloning and expression analysis of a vasa-like gene in Pacific bluefin tuna Thunnus orientalis

Changes in the expression of pituitary gonadotropin subunits during reproductive cycle of multiple spawning female chub mackerel Scomber japonicus

Patterns of oocyte development in natural habitat and captive Salminus hilarii Valenciennes, 1850 (Teleostei: Characidae)

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