Histological Study on the Effect of Adipose Mesenchymal Stem Cells Derived Microvesicles and the Role of its RNA Content on Experimentally-Induced Ulcerative Colitis in Albino Rats

Abstract

Background: Ulcerative colitis (UC) is a bowel inflammatory illness, which shows remission and deterioration episodes of abdominal pain and bloody diarrhea. Nowadays, treatment options are not satisfactory other than their various complications. Adipose tissue derived mesenchymal stem cell microvesicles (MSC-MVs) are considered an emerging promising alternative therapeutic agent. Aim of the work: Evaluation of the therapeutic potential of MSC-MVs on acetic acid (AA) induced UC in addition to the potential role of its RNA content. Materials and Methods: Forty adult male albino rats were equally divided to 4 groups: group I (control), group II (colitis), group III (MVs treated) and group IV (RNase-MVs treated). Colitis was induced by intracolonic AA enema for groups II, III and IV. On day three after enema a single intravenous injection with either PBS, MSC-MVs or RNase treated MSC-MVs was administered to groups II, III and IV, respectively. Seven days later, colon specimens were harvested and cut equally into two parts. The proximal parts were used for biochemical study to measure myeloperoxidase enzyme (MPO) levels. The distal parts were processed for H&E, Alcian blue, iNOS and COX-2 immunohistochemistry. Morphometric and statistical analysis were done. Results: Group III showed improved histological features of UC and marked increase in the mean area percent and optical density of mucin. Also, it showed significant decrease in iNOS and COX-2 immunoreaction and MPO levels. Conclusion: MSC-MVs administration improved UC features. RNA content of the vesicles proved to have a crucial role in such protective effects. Consequently, MSC-MVs could be a promising tool in UC treatment.

Therapeutic role of adipose tissue–derived stem cells versus microvesicles in a rat model of cerebellar injury

“…Specimens were removed 1 week after MV administration. 25 Three rats died (2 rats from group II and one rat from group IV). They were excluded from the experiment.…”

“…On the 11th day, each rat received a single dose of 200 μg MVs diluted in 1 ml of PBS, intravenous through the caudal vein. Specimens were removed 1 week after MV administration 25 . Three rats died (2 rats from group II and one rat from group IV).…”

“… 24 Thus, MVs are promising therapeutic approach for various pathological conditions such as neural degeneration, liver fibrosis, kidney injury and myocardial infarction. 25 Thus, the purpose of our work was to compare the therapeutic effect of ASCs versus MVs on the cerebellar damage induced by MSG.…”

“…Preclinical animal models showed that MSC‐derived MVs (MSC‐MVs) display pro‐angiogenic and cellular protective effects and can be used for tissue regeneration 24 . Thus, MVs are promising therapeutic approach for various pathological conditions such as neural degeneration, liver fibrosis, kidney injury and myocardial infarction 25 . Thus, the purpose of our work was to compare the therapeutic effect of ASCs versus MVs on the cerebellar damage induced by MSG.…”

Comparing the Therapeutic Potential of Mesenchymal Stem Cells- Derived Microvesicles and Infliximab in Chronic Pancreatitis Induced in Rats (Histological and Biochemical Study)

“…Group III (Microvesicles-treated): After induction of chronic pancreatitis, rats received single IV injection of 200μg PKH26 labeled MSCs-MVs diluted with 1ml phosphate buffered saline (PBS) via tail vein, then they were sacrificed after 3 weeks [14] .…”