Histological validation of adipogenic differentiation potential of ASC on collagen-based 2D scaffolds

Savadori, Paolo; Mariano, Sara; Cipolla, Laura; Lombardi, Giovanni

doi:10.1007/s00418-020-01902-9

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No Time To "Clam Up" About Bivalve Germ Granule Assembly1

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2023

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Cited by 2 publications

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“…Interest in human adipose tissue-derived mesenchymal stem cells (ASCs) has been high for many years due to their ability to differentiate into numerous cell types during cell culture (Dai et al 2016). Various types of matrices and scaffolds for cell culture have been tested to examine the potential for ASCs to differentiate into different cell lineages (Gomarasca et al 2020). Similarly, the effects of the composition of the conditioned culture media on lineage differentiation of ASCs have been well studied.…”

Section: No Time To "Clam Up" About Bivalve Germ Granule Assemblymentioning

confidence: 99%

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Taatjes

Roth

2021

Histochem Cell Biol

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Section: No Time To "Clam Up" About Bivalve Germ Granule Assemblymentioning

confidence: 99%

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Taatjes

Roth

2021

Histochem Cell Biol

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Arduino Automated Microwave Oven for Tissue Decalcification

et al. 2023

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Decalcification of hard tissues such as bone and teeth is a complex process that requires using chemicals such as acids and chelating agents. Acids act faster than chelating agents, but they have a greater risk of damaging biological samples. Increasing the reaction speed of the chelating agent may solve this issue. There are several strategies to speed up this process, and using microwaves seems to be one of the most effective. However, lab-dedicated microwave ovens are expensive, and their purchase may seem unjustified. Therefore, a low-cost modification of a commercial microwave oven, consisting of an Arduino automation device, has been developed. The setup has proven reliable for continuous work, thanks to implementing an electronic safety circuit. In addition, it may reduce the decalcification time using a chelating agent, achieving optimal results regarding tissue preservation and quality of histological sections.

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Histological validation of adipogenic differentiation potential of ASC on collagen-based 2D scaffolds

Cited by 2 publications

References 16 publications

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In focus in HCB

Arduino Automated Microwave Oven for Tissue Decalcification

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