Histological Variations of Jejunal and Ileal Mucosa on Days 8 and 15 after Hypophysectomy in Rat: Morphometrical Analysis in Light and Electron Microscopy

Bastie, M. J.; Balas, D; Laval, Jacques; Senegas-Balas, F.; Bertrand, C.; Frexinos, J; Ribet, A

doi:10.1159/000145525

Cited by 21 publications

(22 citation statements)

References 19 publications

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“…18]. Thus repeated injections of somatostatin probably inhibit the intracellular synthesis of gastrin since we have never noticed any variations in light granules which would indicate associated modifications of the hormonal secretion [2]. But a more direct action of somatostatin might also be suspected as an early and significant de crease of labelling indexes was observed 2 h after the first injection of somatostatin.…”

Section: Discussionmentioning

confidence: 73%

Long-Term Effect of Somatostatin 14 on Mouse Stomach, Antrum, Intestine and Exocrine Pancreas

Senegas-Balas¹,

Balas²,

Pradayrol³

et al. 1985

Cells Tissues Organs

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Mice were injected 3 times a day for 12 days with 8 µg/kg of somatostatin 14 which caused ahypoplasia of parietal and goblet cells, a hypotrophy and hypofunctionality of pancreatic acinar cells with a decrease in lipase and chymotrypsin activities, a decrease in the secretory function of the Brunner gland and in the number of dark granules of G cells. Neither villous and microvillous areas nor brush border hydrolase activities were affected. The number of peptic cells and Paneth cells increase as the level of pepsin and lysozyme. Mice were injected 4 times per hour with 2 µg/kg of somatostatin. 2 h after the first injection of somatostatin and 90 min after a single injection of tritiated thymidine, fundic, antral, jejunal and ileal labelling indexes strongly decrease (maximal effect in ileum). The inhibitory effect of somatostatin on the digestive epithelial cell proliferation compared to its long-term action only directed on specific cell types evokes probable compensatory mechanisms induced to maintain the equilibrium of the digestive epithelia.

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Section: Discussionmentioning

confidence: 73%

Long-Term Effect of Somatostatin 14 on Mouse Stomach, Antrum, Intestine and Exocrine Pancreas

Senegas-Balas¹,

Balas²,

Pradayrol³

et al. 1985

Cells Tissues Organs

Self Cite

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“…Strong evidence has demonstrated that growth hormone (GH) is an important growth factor for intestine. Complete GH depletion due to hypophysectomy caused pronounced hypoplasia of small intestinal mucosa with decreased villus height and reduced crypt cell proliferation [36] . Simple replacement of GH can restore mucosal proliferative activity [37] .…”

Section: Discussionmentioning

confidence: 99%

Effect of bowel rehabilitative therapy on structural adaptation of remnant small intestine: animal experiment

Zhou

Li²,

Li³

et al. 2001

WJG

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AIM To investigate the individual and the combined effects of glutamine, dietary fiber, and growth hormone on the structural adaptation of the remnant small bowel. METHODS Forty-two adult male SpragueDawley rats underwent 85% mid-small bowel resection and received total parenteral nutrition ( T P N ) s u p p o r t d u r i n g t h e f i r s t t h r e e p o s t o p e r a t i o n a l d a y s . F r o m t h e 4 t h postoperational day, animals were randomly assigned to receive 7 different treatments for 8 days: TPNcon group, receiving TPN and enteral 20 g·L -1 glycine perfusion; TPN+Gln group, receiving TPN and enteral 20 g·L -1 glutamine perfusion; ENcon group, receiving enteral nutrition (EN) fortified with 20 g·L -1 glycine; EN+Gln group, enteral nutrition fortified with 20 g·L -1 glutamine; EN+Fib group, enteral nutrition and 2 g·L -1 oral soybean fiber; EN+GH group, enteral nutrition and subcutaneous growth hormone (GH) (0.3IU) injection twice daily; and ENint group, glutamine-enriched EN, oral soybean fiber, and subcutaneous GH injection. RESULTS Enteral glutamine perfusion during TPN increased the small intestinal villus height (jejunal villus height 250 µm ± 29 µm in TPNcon vs 330 µm ± 54 µm in TPN+Gln, ileal villus height 260 µm ± 28 µm in TPNcon vs 330 µm ± 22 µm in TPN+Gln, P<0.05) and mucosa thickness (jejunal mucosa thickness 360 µm ± 32 µm in TPNcon vs 460 µm ± 65 µm in TPN+Gln, ileal mucosa thickness 400µm ± 25 µm in TPNcon vs 490 µm ± 11 µm in TPN+Gln, P<0.05) in comparison with the TPNcon group. Either fiber supplementation or GH administration improved body mass gain (end body weight 270 g ± 3.6 g in EN+Fib, 265.7 g ± 3.3 g in EN+GH, vs 257 g ± 3.3 g in ENcon, P<0.05), elevated plasma insulinlike growth factor (IGF-I) level (880 µg·L -1 ± 52 µg·L -1 in EN+Fib, 1 200 µg·L -1 ± 96 µg·L -1 in EN ± GH, vs 620 µg·L -1 ± 43 µg·L -1 in ENcon, P<0.05), and increased the villus height (jejunum 560 µm ± 44 µm in EN ± Fib, 530 µm ± 30 µm in EN ± GH, vs 450 µm ± 44 µm in ENcon, ileum 400 µm ± 30 µm in EN+Fib, 380 µm ± 49 µm in EN ± GH, vs 320 µm ± 16 µm in ENcon, P<0.05) and the mucosa thickness (jejunum 740 µm ± 66 µm in EN ± Fib, 705 µm ± 27 µm in ENGH, vs 608 µm ± 58 µm in ENcon, ileum 570 µm ± 27 µm in EN ± Fib, 560 µm ± 56 µm in EN ± GH, vs 480 µm ± 40 µm in ENcon, P<0.05) in remnant jejunum and ileum. Glutamine-enriched EN produced little effect in body mass, plasma IGF-I level, and remnant small bowel mucosal structure. The ENint group had greater body mass (280 g ± 2. 2 g), plasma IGF-I level (1450 µg·L -1 ± 137 µg·L -1 ), and villus height (jejunum 620 µm ± 56 µm, ileum 450 µm ± 31 µm) and mucosal thickness (jejunum 800 µm ± 52 µm, ileum 633 µm ± 33 µm) than those in ENcon,EN+Gln (jejunum villus height and mucosa thickness 450 µm ± 47 µm and 610 µm ± 63 µm, ileum villus height and mucosa thickness 330 µm ± 39 µm and 500 µm ± 52 µm), EN+GH groups (P<0.05), and than those in EN+Fib group although no statistical significance was attained. CONCLUSION Both dietary fiber and GH when used separately c...

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“…Strong evidences have demonstrated that growth hormone (GH) was an important growth factor for intestine [25] . Complete GH depletion due to hypophysectomy could cause pronounced hypoplasia of small intestinal mucosa with decreased villus height and reduced crypt cell proliferation [26] . Simple replacement of GH could restore mucosal proliferative activity [27] , rhGH could promote normal growth and development in the body by changing chemical activity in cells.…”

Section: Discussionmentioning

confidence: 99%

Changes of biological functions of dipeptide transporter (PepT1) and hormonal regulation in severe scald rats

Sun

Zhao²,

Wang³

et al. 2003

WJG

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Histological Variations of Jejunal and Ileal Mucosa on Days 8 and 15 after Hypophysectomy in Rat: Morphometrical Analysis in Light and Electron Microscopy

Cited by 21 publications

References 19 publications

Long-Term Effect of Somatostatin 14 on Mouse Stomach, Antrum, Intestine and Exocrine Pancreas

Long-Term Effect of Somatostatin 14 on Mouse Stomach, Antrum, Intestine and Exocrine Pancreas

Effect of bowel rehabilitative therapy on structural adaptation of remnant small intestine: animal experiment

Changes of biological functions of dipeptide transporter (PepT1) and hormonal regulation in severe scald rats

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