Histology and ultrastructure of the fat body of Anticarsia gemmatalis (HÜBNER, 1818) (LEPIDOPTERA: NOCTUIDAE)

Carvalho, Renata Baldo Rodrigues; Andrade, Fábio Goulart de; Levy, Sheila Michele; Moscardi, F.; Falleiros, Ângela Maria Ferreira

doi:10.1590/s1516-89132013000200016

Cited by 11 publications

(7 citation statements)

References 20 publications

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“…Thus, Plodia interpunctella Hübner (Shirk and Malone 1989) shows one dark-colored fat body located in the posterior part of the abdomen, and another whitish one in its anterior part; whereas Helicoverpa zea (Boddie) (Haunerland and Shirk 1995) presents a parietal fat body in the anterior part of the abdomen, its color being lighter than that of the previsceral when treated with hematoxiline-eosine. In Anticarsia gemmatalis Hübner (Carvalho et al 2013), however, two fat bodies, one parietal and one perivisceral, can be observed. The former is underneath the tegument and beside the digestive tube, with an irregular distribution between the thorax-abdomen regions, and the latter is beside the digestive tube.…”

Section: Resultsmentioning

confidence: 97%

Histology and histochemistry of Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) fat body during the post embrionary development

Vaca¹,

Michel²,

Alcaide³

2019

RCHE

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Revista Chilena de Entomología (2019) 45 (4): 521-532.Este es un artículo de acceso abierto distribuido bajo los términos de la licencia Creative Commons License (CC BY NC 4.0) Research Article Histology and histochemistry of Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) fat body during the post embrionary developmentHistología e histoquímica del cuerpo graso de Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) durante el desarrollo postembrionario Abstract. Phyllocnistis citrella (Stainton), is one of the most worrying pests in the citrus growing sector. Insects' fat body is an important tissue where numerous metabolic processes take place; its significance can be clearly seen in its main functions, i.e., assimilation and oxidation of substances so as to obtain energy and generate tissue; beside, it is the main storage source of fats and carbohydrates. The objective of the present work is to analyse its histological and histochemical changes during the post embryonic development of this species. The study material was collected in a lemon growing area free from pesticides. This material was fixed in specific solutions so as to keep proteins, lipids and carbohydrates and was stained with Hematoxylin-Eosin, Schiff Periodic Acid, Nile Blue, Sudan III and Sudan Black. During larval development, the trophocyte cytoplasmatic drops have a lipidic nature. In the prepupa stage, a decrease of lipidic drops and an increase of basophilic granulations and mild PAS positivity in the trophocyte cytoplasm were observed, indicating the beginning of the synthesis and storage of proteins and carbohydrates. Pupa's fat body accumulates protein granules with low content of glycogen. The lipid content decreases with a predominance of acid lipids. Changes in the fat body chemical composition during the post-embryonic development of Phyllocnistis citrella are related to lysis of larval tissues and the formation of adult structures during metamorphosis.Resumen. Phyllocnistis citrella (Stainton) es una de las plagas que más ha preocupado al sector citrícola. El cuerpo graso de los insectos es un importante tejido en el cual ocurren procesos metabólicos, como la utilización de sustancias absorbidas por el intestino, su asimilación y oxidación para la obtención de energía y génesis de tejidos, siendo también la principal fuente de reserva de grasas y carbohidratos. El objetivo del presente trabajo es analizar los cambios histomorfológicos e histoquímicos durante el desarrollo postembrionario de la especie. Las muestras obtenidas de quintas sin aplicación de insecticidas se fijaron en soluciones específicas para la conservación de proteínas, carbohidratos y lípidos. Se coloreo con Hematoxilina-Eosina, Ácido Peryódico Schiff, Azul de Nilo, Sudan III y Sudan Black. Durante el desarrollo larval se identifican trofocitos de aspecto vacuolar con contenido lipídico. En prepupa se observa disminución de vacuolas lipídicas, aumento de granulaciones basófilas y leve PAS positividad en el citoplasma de los trofocitos, confi...

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Section: Resultsmentioning

confidence: 97%

Histology and histochemistry of Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) fat body during the post embrionary development

Vaca¹,

Michel²,

Alcaide³

2019

RCHE

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“…In Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) and Phlebotomus papatasi (Scopoli, 1786) (Diptera: Psychodidae), it was observed that glycogen accumulation was higher in PV fat body cells after feeding the flies with sugar (Assis et al, 2014). Although the PF and PV fat bodies of the caterpillar Anticarsia gemmatalis Hübner, 1818 (Lepidoptera: Erebidae) have different roles, it has been stated that both of them were involved in the synthesis of lipids and other substances (Carvalho et al, 2013). In our study on B. orientalis, we observed that larger lipid droplets were stored in the PF fat body while more protein granules and glycogen were deposited in the PV fat body similar to the results reported by Dean et al (1985) and Haunerland & Shirk (1995).…”

Section: Discussionmentioning

confidence: 99%

“…These are peripheral (PF) fat body located below the integument and perivisceral (PV) fat body, observed around the digestive tract (Resh & Carde, 2003;Lipovsek & Novak, 2016). These two regions can have different lipid, glycogen, and protein densities (Carvalho et al, 2013). It has been suggested that lipid synthesis and storage occur mainly in PF fat body, whereas PV fat body is associated with protein synthesis and storage in various insects (Dean et al, 1985;Roma et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Doğu hamam böceği Blatta orientalis (L., 1758) (Blattodea: Blattidae)’in trofositlerindeki makromoleküllerin histokimyasal ve ince yapı analizi

Zülfikaroğlu¹,

Izzetoğlu

Yıkılmaz

et al. 2023

TED

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The fat body is a major storage area for glycogen, lipid and protein. The trophocyte is the main cell of fat body and stores these macromolecules. The fat body consists of two parts; peripheral and perivisceral. Peripheral fat body is located below the integument while perivisceral fat body is around the digestive tract. The study was conducted in EGEMIKAL Analysis Laboratory and Histology Laboratory of Ege University between 2018 and 2020. The fat body contents of insects at all stages were examined comparatively in three selected sections through histochemical and ultrastructural studies. We identified macromolecules stored in the trophocytes. Both the granular form of proteins and asterisk structure of glycogen localized around the lipid droplets were observed clearly. It was found that accumulation of protein continued in the trophocytes, but glycogen accumulation decreased considerably in adults compared to all nymphal stages. We also found that larger lipid droplets were stored in the PF fat body, while glycogen and protein accumulation was much higher in the PV fat body. These results may contribute to understanding of the mechanisms underlying activities such as amino acid, nitrogen, lipid and carbohydrate metabolism and protein synthesis in insects.

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“…These two regions may differ from each other in shape as well. While the PF fat body is in the form of a cylindrical cell layer, the PV fat body is formed by cell clusters (Carvalho et al, 2013). In B. orientalis , PF fat body possessed one or several rows of cells under the integument, while PV fat body had cell clusters around the midgut, hindgut and gonads.…”

Section: Discussionmentioning

confidence: 99%

Demonstrating the general structure and cell types of the fat body in Blatta orientalis (Oriental Cockroach)

Zülfikaroğlu

Izzetoğlu

Yıkılmaz

et al. 2021

Anat Histol Embryol

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Histology and ultrastructure of the fat body of Anticarsia gemmatalis (HÜBNER, 1818) (LEPIDOPTERA: NOCTUIDAE)

Abstract: The aim of this study was to analyze the morphologically the fatty body of fourth-instar

Cited by 11 publications

References 20 publications

Histology and histochemistry of Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) fat body during the post embrionary development

Histology and histochemistry of Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) fat body during the post embrionary development

Doğu hamam böceği Blatta orientalis (L., 1758) (Blattodea: Blattidae)’in trofositlerindeki makromoleküllerin histokimyasal ve ince yapı analizi

Demonstrating the general structure and cell types of the fat body in Blatta orientalis (Oriental Cockroach)

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