Histology of corpuscles of Stannius in Lake Van fish (Alburnus tarichi Güldenstädt, 1814) (Cyprinidae)

Abstract

In the present study, the location, histology and number of corpuscles of Stannius (Sc), which are endocrine glands associated with the kidneys of teleost fish, were investigated for the first time in Lake Van fish (Alburnus tarichi), an anadromous and endemic inhabiting Turkey's Lake Van Basin. The Sc, which were ovoid or spheroid and white or cream in colour, were found to vary in number between three and five among the examined fish. The glands were located in the caudal part of the kidney, and either partially or completely embedded, and found to be present on both the ventral and dorsal surface of either side of the caudal part of the kidney. The Sc were surrounded by a connective tissue capsule that penetrated and divided the gland into incomplete lobules. Two types of cells were determined in the parenchyma of the gland. Type‐I cells were predominant throughout the parenchyma and larger than the second (type‐II). In the type‐I cells, the cytoplasm was observed as weakly or moderately eosinophilic with haematoxylin and eosin staining and weakly or moderately acidophilic with Mallory's triple staining. In the type‐I cells, the cytoplasm exhibited weak to moderate periodic acid‐Schiff staining and slight or uniform staining with aldehyde fuchsin. The type‐II cells were round, had a darkly stained spherical nucleus and were dispersed among the type‐I cells. They displayed no cytoplasmic staining with the abovementioned stains.

Histochemical changes, cellular dynamics and antioxidant responses in the corpuscles of Stannius of the female Lake Van fish ( Alburnus tarichi Güldenstädt, 1814) (Cyprinidae) during migration from highly alkaline and brackish water to freshwater

“…The morphological and histological appearance of the CS is consistent with the observations described in our previous study (Kaptaner et al, 2021) that 3 to 5 glands are located ventro‐ or dorso‐caudal surface of the kidney and each of which is encapsulated by a connective tissue capsule that divides the gland into incomplete lobules and glandular parenchyma consists of two types of cell type, namely type‐I and type‐II cells based on their sizes and staining patterns with conventional histological stains.…”

“…As previously described, two distinct gland cells are present in the CS of the female Lake Van fish (Kaptaner et al, 2021). PAS staining was weak to moderate in the cytoplasms of predominant type‐I cells.…”

“…As previously described, two distinct gland cells are present in the CS of the female Lake Van fish (Kaptaner et al, 2021). PAS 1980).…”

“…This fish species is biologically appealing and worthy of scientific inquiry due to its ability to adapt to the lake's extreme living conditions and demonstrates spawning migration activity in freshwater. In our previous study (Kaptaner et al, 2021), we examined the anatomical position and histological structure of CS in Lake Van fish, finding that 3 to 5 CS were located on both the ventral and dorsal surfaces of the caudal kidney with two distinct gland cells using PAS and AF stains. Smaller type-II cells with darkly stained nuclei showed no cytoplasmic staining with PAS or AF, whereas the predominant and larger type-I cells showed weak to moderate staining with PAS and little staining with AF.…”

Histology of some major immune system organs in Lake Van fish Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)

“…The kidney of Lake Van fish consisted of two lobes that were attached dorsally to the wall of the thoracoabdominal cavity and divided into three parts: the head, trunk and tail, as described by Kaptaner (2017) and Kaptaner et al. (2021). It was covered by a mesothelium composed of single squamous cells (Figure 5a).…”