Histometric Analysis of the Effect of Enamel Matrix Derivative on the Healing of Periodontal Defects in Rats With Diabetes

Abstract

DM may produce a significant detrimental effect on BD. EMD may provide greater DF under diabetic or normal conditions; however, it may not significantly increase NCF in animals with DM.

Outcome of Enamel Matrix Derivative Treatment in the Presence of Chronic Stress: Histometric Study in Rats

“…At the end of the daily 12‐hour cycle, all animals were returned to their cages. Fifteen days after the beginning of CS (for the G1 group), both groups were subjected to the creation of the fenestration defects as previously described 22,23 . Periodontal fenestration defects (4 mm in width, 3 mm in length, and ≈1 mm deep) were created bilaterally by removing the superficial bone with a round dental bur (diameter 2 mm) at slow speed under saline irrigation.…”

“…Periodontal fenestration defects (4 mm in width, 3 mm in length, and ≈1 mm deep) were created bilaterally by removing the superficial bone with a round dental bur (diameter 2 mm) at slow speed under saline irrigation. With a chisel, the distal root of the first mandibular molar was carefully denuded of its periodontal ligament, cementum, and superficial dentin 22,23 . After defect creation, rats were randomly assigned to two subgroups: non‐treated control and treatment with EMD, § following a split‐mouth design.…”

“…Ten sections per specimen representing the middle portion of the defect (24 μm apart) were selected, and the percentage of defect fill (DF), the density of newly formed bone (bone density [BD)]) and new cementum formation (NCF) were histomorphometrically assessed by a single masked and calibrated examiner (MGC) with the aid of an image analysis system ¶ . The percentages of both BD and DF were obtained by the point‐counting technique 22,23 . A square grid was overlaid on the defect area, constituting a drawing with intersections.…”

“…The values were averaged, and a mean value (in percent) was obtained for each animal for statistical analysis. The percentage of NCF was obtained by the ratio of the extent of new cementum extension and the total extent of the instrumented root, multiplied by 100 22,23 …”

“…Furthermore, a recent systematic review supports the positive effect of the use of EMD in furcations and infrabony defects 21 . The use of EMD under behavioral and systemic factors has been suggested as an alternative to improve the repair of periodontal tissues 22,23 …”