Histomorphological alterations in organs of the plaicePleuronectes obscurus from a polluted part of Peter the Great Bay, Sea of Japan

Syasina, I. G.; Arbuzova, L. L.; Жадько, Е.А.; Sokolovskii, A. S.

doi:10.1007/bf02759508

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…In Amursky Bay, biomonitoring of the marine environment and flatfish health has long been performed (Syasina et al 2000;Syasina and Sokolovskii 2001;Vashchenko et al 2005). Many species of flounders and plaices are subject to chronic anthropogenic pollution (residential and industrial sewage from the city of Vladivostok); as a result, some organs and tissues of flatfishes have accumulated toxic chemical substances, such as DDT and its metabolites, hexachlorocyclohexane and heavy metals (Syasina and Durkina 2008).…”

Section: Introductionmentioning

confidence: 99%

Enzyme-linked immunosorbent assay (ELISA) measurement of vitellogenin in plasma and liver histopathology in barfin plaice Liopsetta pinnifasciata from Amursky Bay, Sea of Japan

Shved

Kumeiko

Syasina

2011

Fish Physiol Biochem

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Vitellogenin (Vg) of the barfin plaice Liopsetta pinnifasciata was isolated and purified. In native polyacrylamide gel electrophoresis, Vg appeared as one band. After being subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), Vg fraction produced several polypeptides with molecular masses of 180, 98, 70, 52, 41 and 37 kDa. MALDI-TOF mass spectrometry (MS) of the 180- and 98-kDa Vg polypeptides from the SDS-PAGE gel and de novo sequencing of their four peptide fragments based on MS/MS analysis confirmed that the purified proteins were vitellogenins, which shared high similarity with the Vgs of the barfin flounder Verasper moseri and Atlantic halibut Hippoglossus hippoglossus. The most part of the predicted sequences obtained from the L. pinnifasciata 180-kDa polypeptide has previously been found in the V. moseri vitellogenin type B, the sequences obtained from the 98-kDa polypeptide were found in V. moseri vitellogenin type A, so these findings allow us to propose that L. pinnifasciata has at least two different forms of Vg. Rabbit polyclonal antibodies against Vg were produced, and a quantitative enzyme-linked immunosorbent assay was developed. The concentration of Vg in barfin plaice from the moderately contaminated area of Amursky Bay in the Sea of Japan was detected based on the maturity stage of their gonads. In November 2008, the Vg concentration in the plasma of females with advanced oogenesis varied from 5.295 to 28.367 mg/ml (mean 16.38 ± 6.73 mg/ml, CV = 41.1%); in the plasma of males, the concentration ranged from non-detectable to 0.957 mg/ml (0.29 ± 0.42 mg/ml, CV = 127.9%). In October 2009, the Vg concentration in female plasma was lower than in November 2008 (2.21-13.87 mg/ml). High individual variability of plasma Vg was characteristic for maturing males (CV = 200.3%) and immature females (CV = 255.5%), and there was no significant difference between plasma Vg concentrations in males captured in November 2008 and October 2009 or in maturing males and immature females. Vacuolisation of hepatocytes was more typical for males with low plasma Vg concentrations and females with high plasma Vg concentrations. Necrosis and pyknosis of hepatocyte nuclei were more frequent in males with high Vg concentrations and in females with low plasma Vg concentrations.

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Section: Introductionmentioning

confidence: 99%

Enzyme-linked immunosorbent assay (ELISA) measurement of vitellogenin in plasma and liver histopathology in barfin plaice Liopsetta pinnifasciata from Amursky Bay, Sea of Japan

Shved

Kumeiko

Syasina

2011

Fish Physiol Biochem

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“…It is believed that the gills of fish exposed to irritants are of the most important organs that are highly affected (Saad et al, 2011). Histological changes have been reported in the liver of fish as a result of exposure to industrial and municipal sewage waste (Syasina et al, 2000), heavy metals (Osman et al, 2009). Histological abnormalities were seen in the muscle of fish as a result of exposure to different toxicants (Abbas andAli, 2007 andOliveira Ribeiro et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Biochemical and histological assessment of some heavy metals on Biomphalaria alexandrina snails and Oreochromis niloticus fish in Lake Burullus, Egypt.

El-Khayat¹,

Abd-Elkawy²,

Ouf³

et al. 2018

Egypt. J. of Aquatic Biolo. and Fish.

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“…Flatfi shes are widely used as bioindicators of contamination of the marine environment [1][2][3][4] including moderate and heavily contaminated parts of Peter the Great Bay, Sea of Japan [5]. In our previous investigations we used endemic speciesbarfi n plaice Liopsetta pinnifasciata and one of the most common species -black plaice Pseudopleuronectes obscurus [6]. The black plaice is a typical inhabitant of the upper subtidal.…”

Section: Introductionmentioning

confidence: 99%

Histopathology of the Black Plaice Pseudopleuronectes Obscurus from a Marine Reference Area

Syasina¹

2017

Int J Aquac Fish Sci

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The Kievka Bay is one of the cleanest regions on the Far East coast of Russia. There are no anthropogenic impacts on the coastal area, and thus the infl uence of pollutants on the state of fi sh health is minimized. Histopathological examination of black plaice was performed to study the background level of morbidity and the occurrence of pathological alterations in tissues of fi shes from this ecologically clean region. Different pathological alterations were detected in fi shes sampled. The most surprising was a disease of black plaice, where necrotic changes were found in the gonads of 28% of the males, and the destructive changes were absent in females. In the gills, pathogenic microorganisms were detected, which induced strong hyperplasia of the respiratory epithelium, fusion of lamellas, and necrosis of pillar cells, in 28% of the individuals. Diseases of the liver were recorded for two fi shes that comprised 8% of the total number, in one case-granulomatous infl ammation. Besides that some fi shes had a diffuse necrosis in the liver. In the kidneys of all sampled fi sh, parasites were present in the lumen of the renal duct, very likely a moderately pathogenic species, as the consequences of parasitism were insignifi cant and for a smaller part of fi shes-moderate. Thus, different pathological alterations were found in all investigated organs, and, in most cases, it was possible to establish their occurrence with parasitism.

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Histomorphological alterations in organs of the plaicePleuronectes obscurus from a polluted part of Peter the Great Bay, Sea of Japan

Cited by 5 publications

References 18 publications

Enzyme-linked immunosorbent assay (ELISA) measurement of vitellogenin in plasma and liver histopathology in barfin plaice Liopsetta pinnifasciata from Amursky Bay, Sea of Japan

Enzyme-linked immunosorbent assay (ELISA) measurement of vitellogenin in plasma and liver histopathology in barfin plaice Liopsetta pinnifasciata from Amursky Bay, Sea of Japan

Biochemical and histological assessment of some heavy metals on Biomphalaria alexandrina snails and Oreochromis niloticus fish in Lake Burullus, Egypt.

Histopathology of the Black Plaice Pseudopleuronectes Obscurus from a Marine Reference Area

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