Histomorphological and biochemical changes induced by triptolide treatment in male lesser bandicoot rat, Bandicota bengalensis

Dhar, Parul; Singla, Neena

doi:10.1016/j.pestbp.2014.09.012

Cited by 9 publications

(5 citation statements)

References 31 publications

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“…Similar observations were made earlier in rats treated with triptolide (Singla et al. 2013; Dhar & Singla 2014b).…”

Section: Resultssupporting

confidence: 88%

“…The slow recovery of spermatogenic cell counts after discontinuation of treatment during the study period may be due to the fact that the cumulative doses of quinestrol in some rats may have reached a critical level before it was too late for the testicular damage or degeneration to become too severe to be completely reversed 60 days after stopping the drug. Similar observations were made earlier in rats treated with triptolide (Singla et al 2013;Dhar & Singla 2014b).…”

Section: Effect Of 003% Quinestrol Treatment In Reducing Rodent Activ...supporting

confidence: 90%

“…2014). Anti‐fertility drugs can cause infertility in both sexes (Dhar & Singla 2014a,b). The most commonly used method of contraception in wild animals is the use of steroid hormones (Massei et al.…”

Section: Introductionmentioning

confidence: 99%

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Antifertility effects of quinestrol in male lesser bandicoot rat, Bandicota bengalensis, and potential in managing rodent population under field conditions

Sidhu

Singla

2023

Integr. Zool.

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Integrating fertility control techniques using steroid hormones after lethal control can help reduce post control rebuildup of rodent populations. The current study is the first to assess the antifertility effects of quinestrol in male lesser bandicoot rat, Bandicota bengalensis which is the predominant rodent pest species in Southeast Asia. Rats in different groups were fed bait containing 0.00%, 0.01%, 0.02%, and 0.03% quinestrol for 10 days in laboratory and evaluated immediately, and 15, 30, and 60 days after treatment discontinuation for effect on reproduction and other antifertility parameters. Effect of 0.03% quinestrol treatment for 15 days was also observed in managing rodent populations in groundnut crop fields. Treatment resulted in average consumption of 19.53 ± 1.80, 67.63 ± 5.50, and 246.67 ± 1.78 mg/kg bwt active ingredient by three treated groups of rats, respectively. No reproduction was observed in female rats mated with male rats treated with 0.03% quinestrol, even 30 days after cessation of treatment. Post‐mortem examination showed a significant (P < 0.0001) effect of treatment on organ weights (testis, cauda epididymis, seminal vesicles, and prostate gland) and different sperm parameters (sperm motility, sperm viability, sperm count, and sperm abnormality) in the cauda epididymal fluid with partial reversibility after 60 days. A significant (P < 0.0001) effect of quinestrol on the histomorphology of testis and cauda epididymis was observed, suggesting its effect on spermatogenesis. Affected cell association and cell count in seminiferous tubules did not fully recover within 60 days of stopping treatment. Evaluation of the effects of quinestrol treatment in groundnut fields showed greater reductions in rodent activity in fields treated with 2% zinc phosphide followed by 0.03% quinestrol treatment as compared to fields treated with 2% zinc phosphide alone. Research concludes that quinestrol has the potential to reduce fecundity and post control rebuildup of B. bengalensis populations, but long‐term studies of the effectiveness of quinestrol under large‐scale field conditions are needed to use it as part of an integrated pest control program for rodents.

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“…Similar observations were made earlier in rats treated with triptolide (Singla et al. 2013; Dhar & Singla 2014b).…”

Section: Resultssupporting

confidence: 88%

Section: Effect Of 003% Quinestrol Treatment In Reducing Rodent Activ...supporting

confidence: 90%

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Antifertility effects of quinestrol in male lesser bandicoot rat, Bandicota bengalensis, and potential in managing rodent population under field conditions

Sidhu

Singla

2023

Integr. Zool.

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“…Triptolide alone has been shown to have significant effects for more than 60 days on male reproductive parameters (Norway rats: Huynh et al. 2000; Bandicota bengalensis : Dhar & Singla 2014). As the main objective for fertility control of most wildlife species is to markedly limit recruitment, females are the targeted sex, with effects in males a bonus (see Jacoblinnert et al.…”

Section: Resultsmentioning

confidence: 99%

Developing fertility control for rodents: a framework for researchers and practitioners

2023

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Fertility control is often heralded as a humane and effective technique for management of overabundant wildlife, including rodents. The intention is to reduce the use of lethal and inhumane methods, increase farm productivity and food security as well as reduce disease transmission, particularly of zoonoses. We developed a framework to guide researchers and stakeholders planning to assess the effectiveness of a potential contraceptive agent for a particular species. Our guidelines describe the overarching research questions which must be sequentially addressed to ensure adequate data are collected so that a contraceptive can be registered for use in broad‐scale rodent management. The framework indicates that studies should be undertaken iteratively and, at times, in parallel, with initial research being conducted on (1) laboratory‐based captive assessments of contraceptive effects in individuals; (2) simulation of contraceptive delivery using bait markers and/or surgical sterilization of different proportions of a field‐based or enclosure population to determine how population dynamics are affected; (3) development of mathematical models which predict the outcomes of different fertility control scenarios; and (4) implementation of large‐scale, replicated trials to validate contraceptive efficacy under various management‐scale field situations. In some circumstances, fertility control may be most effective when integrated with other methods (e.g. some culling). Assessment of non‐target effects, direct and indirect, and the environmental fate of the contraceptive must also be determined. Developing fertility control for a species is a resource‐intensive commitment but will likely be less costly than the ongoing environmental and economic impacts by rodents and rodenticides in many contexts.

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“…Others such as triptolide (TP) have been shown to have medium-term effects in males, and shorter-term effects in females. It impairs spermatogenesis and reduces the diameter of seminiferous tubules as well as sperm motility and viability (Huynh et al 2000;Li et al 2009;Singla & Challana 2014;Dhar & Singla 2014a;Witmer et al 2017). Singla and Challana (2014) found that TP causes severe structural and morphological changes in sperm, such as head-tail separation, a degenerated mitochondria sheath, absent plasma membrane, or the aggregation of sperm tails.…”

Section: Plant Extractsmentioning

confidence: 99%

The status of fertility control for rodents—recent achievements and future directions

et al. 2021

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Management of overabundant rodents at a landscape scale is complex but often required to sustainably reduce rodent abundance below damage thresholds. Current conventional techniques such as poisoning are not species specific, with some approaches becoming increasingly unacceptable to the general public. Fertility control, first proposed for vertebrate pest management over 5 decades ago, has gained public acceptance because it is perceived as a potentially more species-specific and humane approach compared with many lethal methods. An ideal fertility control agent needs to induce infertility across one or more breeding seasons, be easily delivered to an appropriate proportion of the population, be species specific with minimal side-effects (behavioral or social structure changes), and be environmentally benign and cost effective. To date, effective fertility control of rodents has not been demonstrated at landscape scales and very few products have achieved registration. Reproductive targets for fertility control include disrupting the hormonal feedback associated with the hypothalamic-pituitary-gonadal axis, gonad function, fertilization, and/or early implantation. We review progress on the oral delivery of various agents for which laboratory studies have demonstrated efficacy in females and/or males and synthesize progress with the development and/or use of synthetic steroids, plant extracts, ovarian specific peptides, and immunocontraceptive vaccines. There are promising results for field application of synthetic steroids (levonorgestrel, quinestrol), chemosterilants (4-vinylcyclohexene diepoxide), and some plant extracts (triptolide). For most fertility control agents, more research is essential to enable their efficient and cost-effective delivery such that rodent impacts at a population level are mitigated and food security is improved.

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Histomorphological and biochemical changes induced by triptolide treatment in male lesser bandicoot rat, Bandicota bengalensis

Cited by 9 publications

References 31 publications

Antifertility effects of quinestrol in male lesser bandicoot rat, Bandicota bengalensis, and potential in managing rodent population under field conditions

Antifertility effects of quinestrol in male lesser bandicoot rat, Bandicota bengalensis, and potential in managing rodent population under field conditions

Developing fertility control for rodents: a framework for researchers and practitioners

The status of fertility control for rodents—recent achievements and future directions

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