Histomorphological and microanatomical characteristics of the olfactory organ of freshwater carp, Cirrhinus reba (Hamilton)

Abstract

The morphoanatomy, cellular organization, and surface architecture of the olfactory apparatus in Cirrhinus reba (Hamilton) is described using light and scanning electron microscopy. The oval shaped olfactory rosette contained 32 ± 2 primary lamellae on each side of the median raphe, and was lodged on the floor of the olfactory chamber. The olfactory lamellae were basically flat and compactly arranged in the rosette. The olfactory chamber communicated to the outside aquatic environment through inlet and outlet apertures with a conspicuous nasal flap in between. The mid dorsal portion of the olfactory lamellae was characterized by a linguiform process. Sensory and non-sensory regions were distributed separately on each lamella. The sensory epithelium occupied the apical part including the linguiform process, whereas the resting part of the lamella was covered with non-sensory epithelium. The sensory epithelium comprised both ciliated and microvillous receptor cells distinguished by the architecture on their apical part. The non-sensory epithelium possessed mucous cells, labyrinth cells, and stratified epithelial cells with distinctive microridges. The functional importance of the different cells lining the olfactory mucosa was correlated with the ecological habits of the fish examined.

The Influence of Fixatives on the Validity of Histological Preparations of Olfactory Organ in Teleostei

“…For the study of olfactory structures, diff erent methods are used such as light microscopy, scanning electron microscopy, and transmission electron microscopy. Th e most popular fi xatives are 2.5 % glutaraldehyde fi xative (Zielinski & Hara, 1988;Arvedlund et al, 2000Arvedlund et al, , 2007Cobcroft & Pankhurst, 2003;Pashchenko & Kasumyan, 2015, 2017, Carnoy fi xative (Devitsina & Radishcheva, 1989), 70 % ethanol (Chen & Arratia, 1994), formalin (Zeiske et al, 1976;Breucker et al, 1979;Yamamoto et al, 2004;Doldan et al, 2011;Kim &Park, 2016) and Bouin's solution (Jahn, 1972;Camacho et al, 2010;Doldan et al, 2011;Ghosh & Chakrabarti, 2016;Tytiuk et al 2018), Bouin-ethanol solution (Diaz et al, 2002).10 % formalin and Bouin's solution are commonly used for histological studies of olfactory structures in Teleostei.…”