Histomorphological researches on large porous hydroxyapatite cylinder tubes with polylactic acid surface coating in different nonskeletal sites in vivo

Abstract

Porous hydroxyapatite (HA) ceramic cylinder tubes coated with polylactic acid on the exposed surfaces were implanted in four nonskeletal sites (omentum, peritoneum, vastus lateralis, and side of femur). Six months postoperatively, proper amount of Chinese ink was injected to dye the implanting areas. Decalcified and nondecalcified sections were observed under inverted microscope. The results showed that the soft tissues around the HA cylinder tubes in peritoneum, vastus lateralis, and side of femur groups appeared visible black. Some small blacked vascular architectures were also discernible. However in omentum group, only small number of blacked vessels existed. Histological observations indicated that vascularization and ossification occurred in peritoneum, vastus lateralis, and side of femur groups. In omentum group, there was no any sign of vascularization and ossification. A conclusion could be made in this study that excepting bones and muscles, parietal peritoneum could serve as a potential spot for culturing histoengineering hydroxyapatite (HA)-polylactic acid (PLA) ceramic bone substitutes.

A novel porous bioceramics scaffold by accumulating hydroxyapatite spherulites for large bone tissue engineering in vivo. II. Construct large volume of bone grafts

“…Moreover, this sphere‐accumulation design can be applied to fabricate scaffolds of controllable hierarchical structures, well interconnected pores, and suitable sizes for the repair of critical‐sized bone defects. Previous studies observed new bone formation in these scaffolds 6 months after implantation at nonosseous sites . The objective of this study was to investigate the evolution of new bone generation, vascularization and biomechanical properties in large volume of HASA scaffolds cultured in different ectopic sites in vivo .…”

“…The resulting HA sphere‐accumulated (HASA) scaffolds featured interconnected macropores and micropores with controllable structures, including shape, size, and hierarchical assembly . Preliminary studies observed the formation of bone tissues in HASA scaffolds six months after implantation at nonosseous sites . In this study, HASA scaffolds were implanted into two non‐osseous sites in dogs (i.e., the abdominal cavity and dorsal muscle) to prepare in vivo TE bone grafts.…”

“…We have previously developed a new scaffold design by accumulating HA spheres in a porous HA tube. [25][26][27] The resulting HA sphere-accumulated (HASA) scaffolds featured interconnected macropores and micropores with controllable structures, including shape, size, and hierarchical assembly. 25 Preliminary studies observed the formation of bone tissues in HASA scaffolds six months after implantation at nonosseous sites.…”

“…25 Preliminary studies observed the formation of bone tissues in HASA scaffolds six months after implantation at nonosseous sites. 26,27 In this study, HASA scaffolds were implanted into two non-osseous sites in dogs (i.e., the abdominal cavity and dorsal muscle) to prepare in vivo TE bone grafts. The evolution of new bone generation, vascularization, and biomechanical property of scaffolds implanted at the two sites were compared.…”

Controlled degradation pattern of hydroxyapatite/calcium carbonate composite microspheres

“…Spherical hydroxyapatite (HAP) is recognized as a preferable biomaterial because of its chemical similarities to inorganic composition of natural bone, excellent biocompatibility, bioactivity, and osteoconductivity (G€ uler et al, 2011;Hannink and Chris Arts 2011 ;Wang et al, 2014) and better flowability (Guo et al, 2009;Li and Bing, 2013;Wu et al, 2013;Zhang et al, 2012). Many organics have been selected to fabricate HAP with modulated forms to meet the requirement.…”