Histomorphology and Histochemistry of the Oviduct in Laying Turkey Hens with Emphasis on the Sperm Host Glands

Nabil, Taghreed Mohamed; Hassan, Randa M.; Mahmoud, HebatAllah H.; Tawfiek, Mohamed Gomaa; Moawad, Usama Kamal

doi:10.17582/journal.aavs/2022/10.5.1076.1089

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Formation and maintenance of SSTs throughout the laying cycle involves cytoskeletal remodeling and extracellular matrix organization, including actin microfilament formation and chondrocyte proliferation ( Mendonca et al, 2019 ; Nabil et al, 2022 ). Cytoskeletal remodeling and extracellular matrix organization regulate other physiological processes, such as angiogenesis, that are important for nutrient delivery.…”

Section: Discussionmentioning

confidence: 99%

Turkey hen sperm storage tubule transcriptome response to artificial insemination and the presence of semen

Brady,

Krasnec,

Hanlon

et al. 2024

Front. Physiol.

View full text Add to dashboard Cite

Introduction: Sperm storage within the uterovaginal junction (UVJ) of avian species occurs in specialized structures termed sperm storage tubules (SSTs) and allows for prolonged storage of semen, though the molecular mechanisms involved in semen preservation are not well understood. Little work has been done examining how function of the SSTs is impacted by insemination and by semen present in the SSTs.Methods: Transcriptome analysis was performed on isolated SSTs from turkey hens receiving no insemination (control), sham-insemination, or semen-insemination at three timepoints (D1, D30, and D90 post-insemination). Bioinformatic and functional annotation analyses were performed using CLC Genomics Workbench, Database for Annotation, Visualization, and Integrated Discovery (DAVID), and Ingenuity Pathway Analysis (IPA). Pairwise comparisons and k-medoids cluster analysis were utilized to decipher differential expression profiles in the treatment groups.Results: The SST transcriptome of the semen inseminated group exhibited the greatest differences within the group, with differences detectable for up to 90 days post insemination, while control and sham-inseminated groups were more similar. In the semen-inseminated samples, upregulation of pathways relating to classical and non-classical reproductive signaling, cytoskeletal remodeling, physiological parameters of the local UVJ environment, and cellular metabolism was observed. In the sham-inseminated samples, upregulation of immune pathways and non-reproductive endocrine hormones was observed.Discussion: This work provides insights into the molecular level changes of the SST in response to insemination as well as to the presence of semen. Results from this study may have direct implications on fertility rates as well as potential strategies for avian semen cryopreservation protocols.

show abstract

Section: Discussionmentioning

confidence: 99%