Histomorphometric analysis of the phenotypical differentiation of recruited macrophages following subcutaneous implantation of an allogenous acellular dermal matrix

Mueller, Cornelia Katharina; Schultze‐Mosgau, Stefan

doi:10.1016/j.ijom.2010.10.025

Cited by 27 publications

(21 citation statements)

References 32 publications

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“…We did note that morphologically, ED2+ cells were usually larger and more elongated than ED1+ cells, and this is consistent with their maturation from smaller, rounder and more monocyte-like ED1+ cells. Recently, ED2 has also been used to identify M2 activation in vivo [Badylak et al, 2008;Brown et al, 2009;Mueller and Schultze-Mosgau, 2011]. Although its target antigen CD163 has been shown to be clearly upregulated in vitro with M2-inducing stimuli, including IL-10 and glucocorticoids [Gordon, 2003], data supporting its use as an M2 marker in vivo is lacking.…”

Section: Discussionmentioning

confidence: 99%

“…Since a handful of studies in the 1990s and early 2000s where macrophage responses to several synthetic materials in the rat were investigated using ED1 and/or ED2 antibodies [Vince et al, 1991;Mohanty et al, 1992;Rosengren et al, 1997;Hagerty et al, 2000;van Luyn et al, 2001;Kidd et al, 2002], little has been published using phenotypic markers identified more recently as understanding of macrophage heterogeneity has increased. Most recent work in rat implant models using multiple markers to characterise M1 and M2 macrophage responses has been on naturally derived scaffolds [Badylak et al, 2008;Brown et al, 2009;Mueller and Schultze-Mosgau, 2011;Brown et al, 2012a;Keane et al, 2012].…”

Section: Introductionmentioning

confidence: 99%

“…M1 macrophages, also designated as pro-inflammatory, or 'classically activated', are well-defined and characterised, and have been labelled in the rat using markers such as CD80, CCR7 and inducible nitric oxide synthase (iNOS) [Badylak et al, 2008;Brown et al, 2009;Mueller and Schultze-Mosgau, 2011;Brown et al, 2012a;Keane et al, 2012;van Putten et al, 2013]. The classification and characterisation of M2, or non-classically activated macrophages, on the other hand is still evolving.…”

Section: Introductionmentioning

confidence: 99%

“…Although M2 and alternative terms have often been used interchangeably, it has been well argued [Gordon, 2003;Stout, 2010] that the latter should be reserved for the M2a subset as coined by Stein et al [1992] to describe the IL-4-driven phenotype, and we will use this nomenclature here. 'M2 markers' in rat in vivo studies have included CD163, arginase-1 and mannose receptor (MR)/ CD206 [Badylak et al, 2008;Brown et al, 2009;Mueller and Schultze-Mosgau, 2011;Ahn et al, 2012;Brown et al, 2012a;Keane et al, 2012;van Putten et al, 2013]. It is widely acknowledged that although the M1/M2 distinction provides a useful conceptual framework for macrophage polarisation [Mantovani et al, 2004], segregation of phenotypes into two distinct subcategories is a major oversimplification [Brown et al, 2012b].…”

Section: Introductionmentioning

confidence: 99%

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Macrophage Phenotype in Response to Implanted Synthetic Scaffolds: An Immunohistochemical Study in the Rat

Palmer

Abberton

Mitchell

et al. 2014

Cells Tissues Organs

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Macrophages predominate among the cells that directly interact with biomaterials and are key orchestrators of host-biomaterial interactions. However, the macrophage response to synthetic scaffolds in particular has not been well studied. The aim of this study was therefore to characterise the macrophage response to several synthetic scaffolds in the rat using immunohistological techniques for a panel of markers of macrophage subclass or activation, including ED1 (CD68), ED2 (CD163), CD80, mannose receptor and inducible nitric oxide synthase (iNOS). Materials were implanted subcutaneously and collected after 6-8 weeks during the chronic phase of the host response. Unmodified polycaprolactone scaffolds uniquely demonstrated a total lack of both macrophage adherence to surfaces and a wider foreign body response compared to scaffolds composed of poly(lactic-co-glycolic acid) (PLGA) and polyurethanes (PURs), with those macrophages present having a clear M2 (MR+, CD80-, iNOS-) phenotype. PLGA scaffolds displayed an M1-dominant (CD80+, iNOS+, MR-) response with substantial foreign body giant cell (FBGC) formation, whilst PUR scaffold FBGCs had a more mixed M1 (CD80+, iNOS+) and M2 (MR+) phenotype. The study also identified that the use of the ED1 antibody in the rat as a pan-macrophage marker is problematic as there is a separate and substantial ED2-positive macrophage population that it does not label, both in response to biomaterials and in normal tissues. The biomaterial-dependent nature of activation for both macrophages and FBGCs was confirmed, and nuanced M1/M2 phenotypes were described.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Macrophage Phenotype in Response to Implanted Synthetic Scaffolds: An Immunohistochemical Study in the Rat

Palmer

Abberton

Mitchell

et al. 2014

Cells Tissues Organs

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show abstract

“…M1 M s participate in the Th1 in ammatory responses primarily through production of pro-inflammatory mediators and up-regulation of cell surface molecules necessary for antigen presentation to activate Th1 cells and enhance the ability to phagocytose pathogenic material. (19,20) M2-type M s, typically induced by Th2 cytokines, mediate resistance to parasites, mediate immune regulation and tissue repair. (4,21) M2 M s release high levels of IL-10 and low levels of IL-12 and IL-23.…”

Section: Origin Subpopulation and Polarization Of Tammentioning

confidence: 99%

Targeting tumor-associated macrophages by anti-tumor Chinese materia medica

Sun

Gao

et al. 2017

Chin. J. Integr. Med.

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Infiltration of the tumor microenvironment (TME) by immune and inflammatory cells is involved in promoting tumorigenesis, tumor progression, local invasion and metastasis.(1) Tumor-associated macrophages (TAMs), a prominent and abundant inflammatory component of solid and hematological malignancies, express a pro-and anti-tumor effect in all stages of carcinogenesis. Asian region. According to the theory of Chinese medicine (CM) and its practice, some Asian region. According to the theory of Chinese medicine (CM) and its practice, some prescriptions of CM regulate the body's internal environment possibly including the prescriptions of CM regulate the body's internal environment possibly including the remodeling the tumor microenvironment (TME). Here we brie y summarize the pivotal remodeling the tumor microenvironment (TME). Here we brie y summarize the pivotal effects of TAMs in shaping the TME and promoting tumorigenesis, invasion, metastasis effects of TAMs in shaping the TME and promoting tumorigenesis, invasion, metastasis and immunosuppression. Furthermore, we illustrate the effects and mechanisms of CMM targeting TAMs in antiand immunosuppression. Furthermore, we illustrate the effects and mechanisms of CMM targeting TAMs in antitumor therapy. Finally, we reveal the CMM's dual-regulatory and multi-targeting functions on regulating TAMs, tumor therapy. Finally, we reveal the CMM's dual-regulatory and multi-targeting functions on regulating TAMs, and hopefully, provide the theoretical basis for CMM clinical practice related to cancer therapy. and hopefully, provide the theoretical basis for CMM clinical practice related to cancer therapy.

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Fucoidan inhibits tooth movement by promoting restorative macrophage polarization through the STAT3 pathway

Zhang

Jin

et al. 2020

Journal Cellular Physiology

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Retention after treatment and effective anchorage control are two essential factors in orthodontics. Our study aimed to explore the effects of fucoidan on orthodontic tooth movement (OTM) and the involvement of macrophages. We established a murine OTM model to test the effect of fucoidan administration. We found that mice injected with fucoidan had a deceleration in OTM and a higher bone mineral density.Moreover, fucoidan increased the proportion of F4/80 + CD206 + macrophages and promoted the messenger RNA expression of Arg-1, CD206, and IL-10 at both in vivo and in vitro levels. In addition, macrophages showed lower expression of TNF-α, IL-1β, and IL-6 and a decrease in F4/80 + CD11c + cells. Mechanistically, the level of phosphorylated STAT3 was elevated in unpolarized and restorative macrophages after treatment with fucoidan. Taken together, our findings suggest that fucoidan treatment inhibits OTM and enhances the stability of teeth after movement by promoting restorative macrophages through the STAT3 pathway.

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Histomorphometric analysis of the phenotypical differentiation of recruited macrophages following subcutaneous implantation of an allogenous acellular dermal matrix

Cited by 27 publications

References 32 publications

Macrophage Phenotype in Response to Implanted Synthetic Scaffolds: An Immunohistochemical Study in the Rat

Macrophage Phenotype in Response to Implanted Synthetic Scaffolds: An Immunohistochemical Study in the Rat

Targeting tumor-associated macrophages by anti-tumor Chinese materia medica

Fucoidan inhibits tooth movement by promoting restorative macrophage polarization through the STAT3 pathway

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