Histomorphometric comparison of the human, swine, and ovine collecting systems

Abstract

The ovine kidney has been recently determined to be a better model than the swine kidney for the study of collecting system healing after partial nephrectomy. However, there is no histological study comparing the collecting systems of these species. To compare human, swine, and ovine collecting systems using histomorphometry. The collecting systems of 10 kidneys from each species (human, swine, and ovine) were processed for histomorphometry. The thickness of the three layers (mucosal connective tissue, submucosal muscular, and adventitial connective tissue) were measured. The densities of smooth muscle fibers, elastic system fibers, and cells were also measured. Additionally, blood vessel density in the adventitial connective tissue was measured. Analysis of the collecting systems from the three species presented several differences. The adventitial connective tissue from the swine samples was thicker, with more blood vessels and smooth muscle fibers, compared with that from the human and ovine samples. Swine also had higher density of elastic fibers on the submucosal muscular layer. Ovine and human collecting systems shared several similar features, such as blood vessel and elastic fiber density in all layers and the density of cellular and muscular fibers in the submucosal muscular and adventitial connective tissue layers. The collecting system of the ovine kidney is more similar to that of the human kidney compared with that of the swine kidney. This may explain the differences between the healing mechanisms in swine and those in humans and sheep after partial nephrectomy. Anat Rec, 299:967-972, 2016. V C 2016 Wiley Periodicals, Inc.Key words: animal models; collecting system; healing; kidney; partial nephrectomySwine has been considered the best animal model for studying partial nephrectomy, because this species bears anatomical and physiological similarities to humans (Sampaio et al., 1998;Yang et al., 2009;de Souza et al., 2011). However, it has been reported that healing in the swine kidney is quite different from the clinical setting in humans. No urinary leakage was observed in swine after laparoscopic partial nephrectomy without collecting system closure (Ames et al., 2005;de Souza et al., 2011), whereas urinary leakage occurred in up to 5.5% of patients, regardless of the collecting system closure method (Stephenson et al., 2004

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