Histomorphometric, fractal and lacunarity comparative analysis of sheep (Ovis aries), goat (Capra hircus) and roe deer (Capreolus capreolus) compact bone samples

Gudea, Alexandru; Ștefan, Andrei

doi:10.5603/fm.2013.0039

Cited by 12 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 The histological structure of bones of ruminants such as the elk (Cervus canadensis), red deer (Cervus elaphus), and roe deer (Capreolus capreolus), where domestic individuals do not exist, was described in previous studies. [4][5][6] In the present work, the results obtained from a wild subspecies are compared for the first time with those from the corresponding domestic subspecies in order to high- Moreover, the femur of sheep brings on some interest because it represents an excellent experimental model to better understand the development and evolution of some human bone diseases, such as osteoporosis and fracture. [7][8][9][10] Finally, the comparison between mouflon and sheep here proposed may represent an example of recognizing bones from different animals, which may be useful in forensic exam and archaeozoological studies.…”

Section: Introductionmentioning

confidence: 98%

Comparative histology of the femur between mouflon (Ovis aries musimon) and sheep (Ovis aries aries)

et al. 2014

View full text Add to dashboard Cite

Mouflon (Ovis aries musimon) and sheep (Ovis aries aries) are considered as the wild and domestic subspecies of the same species. A comparative study on the microstructure of mouflon and sheep femoral bone diaphysis is here reported. Bone microstructure is described for the first time in the mouflon. More than 200 secondary osteons from both subspecies were analyzed and qualitative evaluation was followed by quantitative determination of perimeter, area, minimum and maximum diameters of secondary osteons and Haversian canals. The basic structural patterns observed in both subspecies can be classified as plexiform and irregular Haversian tissue, in accordance with what reported in the literature for most ruminants. The presence of many secondary osteons in the mouflon means that the bone also consists of dense Haversian bone tissue. Statistical analysis demonstrated that mouflon secondary osteons are larger than in the sheep and made of a greater number of lamellae. Since mouflon and sheep are taxonomically closely related and their body size is very similar, the qualitative and quantitative differences here reported could be primarily explained on account of their different lifestyle. Indeed, the habits of wildlife typical of mouflons may lead to the presence of wide areas of dense Haversian tissue in that subspecies, as mechanical stresses are known to be related to number and size of secondary osteons. Finally, this analysis could provide a useful tool to recognize bones from different species, in forensic exam and archaeozoological studies as well.

show abstract

Section: Introductionmentioning

confidence: 98%

Comparative histology of the femur between mouflon (Ovis aries musimon) and sheep (Ovis aries aries)

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Some parameters of the texture analysis are included in the conventional µCT software's, and others can be acquired using plugins and download free software's, like Image J (NIH). This analysis evaluates local variations in gray levels, the degree of complexity, given by numerical values as how a structure fills up the space; and quantifies the emptiness, by an indicator of the degree of gap distribution over a certain surface (30,31). The most used additional analysis on µCT are the degree of anisotropy, fractal dimension, and lacunarity, disposable in the evaluation software, except for the lacunarity.…”

Section: Additional Analysis and "Unthresholded" Methodsmentioning

confidence: 99%

Use of Micro-Computed Tomography for Bone Evaluation in Dentistry

et al. 2018

View full text Add to dashboard Cite

Micro computed tomography (µCT) follows the same principle of computed-tomography used for patients, however providing higher-resolution. Using a non-destructive approach, samples can be scanned, and each section obtained is used to build a volume using tridimensional reconstruction. For bone analysis, it is possible to obtain information about the tissue's microarchitecture and composition. According to the characteristics of the bone sample (e.g. human or animal origin, long or irregular shape, epiphysis or diaphysis region) the pre-scanning parameters must be defined. The resolution (i.e. voxel size) should be chosen taking into account the features that will be evaluated, and the necessity to identify inner structures (e.g. bone channels and osteocyte lacunae). The region of interest should be delimited, and the threshold that defines the bone tissue set in order to proceed with binarization to separate the voxels representing bone from the other structures (channels, resorption areas, and medullary space). Cancellous bone is evaluated by means of the trabeculae characteristics and their connectivity. The cortex is evaluated in relation to the thickness and porosity. Bone mineral density can also be measured, by the amount of hydroxyapatite. Other parameters such as structure-model-index, anisotropy, and fractal dimension can be assessed. In conclusion, intrinsic and extrinsic determinants of bone quality can be assessed by µCT. In dentistry, this method can be used for evaluating bone loss, alterations in bone metabolism, or the effects of using drugs that impair bone remodeling, and also to assess the success rate of bone repair or surgical procedures.

show abstract

“…During the past 20 years, the fractal concept has improved our understanding of many appearances in biological systems. Fractal analysis is a well-established method for the quantification of geometrical complexity in biological and biophysical systems [15,22,35]. It is widely applied to investigate the metabolic rate, population genetics, tissue organisation and tumour growth.…”

Section: Introductionmentioning

confidence: 99%

Fractal and stereological analyses of insulin-induced rat exocrine pancreas remodelling

Pajevic¹,

Aleksić

Golić

et al. 2018

Folia Morphol

View full text Add to dashboard Cite

show abstract

Histomorphometric, fractal and lacunarity comparative analysis of sheep (Ovis aries), goat (Capra hircus) and roe deer (Capreolus capreolus) compact bone samples

Cited by 12 publications

References 20 publications

Comparative histology of the femur between mouflon (Ovis aries musimon) and sheep (Ovis aries aries)

Comparative histology of the femur between mouflon (Ovis aries musimon) and sheep (Ovis aries aries)

Use of Micro-Computed Tomography for Bone Evaluation in Dentistry

Fractal and stereological analyses of insulin-induced rat exocrine pancreas remodelling

Contact Info

Product

Resources

About