Histomorphometric, Immunohistochemical and Microtomographic Comparison between Autogenous and Xenogenous Bone Blocks for Mandibular Lateral Augmentation in Rabbits

Silva, Erick Ricardo; Balan, Vitor Ferreira; Botticelli, Daniele; Soldini, Claudio; Okamoto, Roberta; Xavier, Samuel Porfírio

doi:10.3390/ma14206049

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…Similar to the present study, in that experiment, the bone grew within the graft onto the trabeculae, up to the surface. In another rabbit experiment [34], equine block grafts were compared with autogenous block grafts. While the two showed similar percentages of newly formed bone, volume maintenance and graft incorporation were better in the autologous compared to the xenogeneic graft.…”

Section: Discussionmentioning

confidence: 99%

“…As a viable alternative for replacing autogenous bone, the xenogeneic graft has been used in bone volume augmentation procedures [31][32][33][34][35][36], as it presents clinical advantages such as a decrease in surgical morbidity, greater technical ease, and the ability to conform and adapt the block following the anatomy of the recipient bed [31,[37][38][39]. To install the graft in the recipient bed, some surgical techniques can be used, such as bone grafts in onlay blocks, osteogenic distraction, and particulate bone grafts associated with titanium meshes or collagen membranes [39].…”

Section: Introductionmentioning

confidence: 99%

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Histological Comparison of Collagenated Cancellous Equine Bone Blocks Used as Inlay or Onlay for Lateral Bone Augmentation in Rabbits

Sakaguchi,

Xavier,

Morinaga

et al. 2023

Materials

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Background: The conformation of the recipient site for an inlay graft presents an increased contact with the parent bone compared to an onlay graft. This might favor bone growth within the inlay compared to onlay grafts. Hence, the objective of this study was to compare the bone incorporation and remodeling processes of xenogeneic en bloc grafts placed using two bone grafting techniques, i.e., onlay vs. inlay. Methods: In this prospective, randomized, split-mouth study (test and control sides in the same animal), two bone grafting techniques were comparatively evaluated. The lateral aspect of the rabbit mandible was used as the recipient site, bilaterally. On one side of the mandible, the cortical bone was perforated with drills to allow a better bone formation from the bone wound and the marrow spaces. A xenogeneic bone block was fixed in the center of the prepared region, representing the onlay site. On the other side of the mandible, a 7 mm wide and 3 mm deep circumferential defect was prepared using trephines and drills. A xenogeneic bone block was fixed in the center of the defect, representing the inlay site. Two healing periods were applied in the study: 2 and 10 weeks, each represented by 10 rabbits (n = 10 for each period). Results: After 2 weeks of healing, the mean percentage of new bone was 10.4% and 23.3% at the onlay and inlay grafts, respectively (p = 0.022). After 10 weeks of healing, new bone increased to 13.2% at the onlay sites and 25.4% at the inlay sites (p = 0.080). In the 10-week period, the inlay grafts presented a homogeneous growth of new bone in all regions, while in the onlay grafts, low percentages of new bone were observed in the external regions. Conclusion: The percentage of new bone increased faster and was higher in the inlay grafts than in the onlay grafts. This outcome might be related to the self-contained conformation of the recipient site in the inlay group, which offered more sources for new bone formation compared to the one-wall conformation of the recipient sites in the onlay group. The osteoconductive properties of the biomaterial allowed the newly formed bone to reach the most peripheral regions in both groups. The osteoconductive properties of the biomaterial, together with the protection offered by the collagen membrane, allowed marginal closure of the defects by newly formed bone in the inlay group.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histological Comparison of Collagenated Cancellous Equine Bone Blocks Used as Inlay or Onlay for Lateral Bone Augmentation in Rabbits

Sakaguchi,

Xavier,

Morinaga

et al. 2023

Materials

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“…Cele din urmă însă, nu au demonstrat rezultate satisfăcătoare, îndeosebi în cazul unor adiţii mai mari [3]. Aceasta se datorează faptului că materialele respective au doar proprietăţi osteoconductive, care în urma aplicării în situsul operator conduc la o integrare parţială, cu formarea unui ţesut osteoid, format parţial din os, ţesut fibros și particule de grefă incapsulate [4]. Aceste rezultate au condus la dezvoltarea conceptelor de amestec a grefelor xenogene sau alogene cu rumeguș osos autogen (datorită proprietăţilor osteoinductive și osteogenice ale acestuia).…”

Section: Discussionunclassified

“…The latter, however, did not show satisfactory results, especially in the case of larger additions [3]. This is since these materials have only osteoconductive properties, which lead to a partial integration after application, with the formation of an osteoid tissue, partially formed of bone, fibrous tissue and encapsulated graft particles [4]. These results led to the development of concepts for mixing xenogeneic or allogeneic grafts with autogenous bone grafts (due to its osseoinductive and osteogenic properties).…”

Section: Discussionmentioning

confidence: 99%

Guided bone regeneration through autogenous laminated bone blocks

Bolea¹,

Mostovei²

2022

JSM

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Implant–prosthetic rehabilitation of edentulous patients with various forms of bone atrophy is a current challenge in oral implantology. The aim of the study is to evaluate the bone volume obtained after grafting procedures using cortical blocks according to the Khoury technique. Material and methods: The study was focused on 10 patients with atrophies of the alveolar ridges that required procedures to create the bone supply for implant– prosthetic rehabilitation. The latter were performed using the Khoury technique, using laminated blocks installed at a distance from the recipient area, filling the space created with autogenous bone (50% of cases) and in combination with xenograft (50% of cases). Following the analysis of the data calculated on CBCT, the presence of a bone ridge with a thickness of 4.54±0.28mm (at 1mm apical from the top of the ridge) and 6.5±0.4mm (at a depth of 4mm) was determined. In 8 out of 10 patients, the implants were inserted in the same session, due to the possibility of fixing them in the apical part. Results: Following the surgery, the obtained thickness of the ridge was assessed at the same levels determined preoperatively, which constituted on average 9.5±0.26mm (at 1mm subcrestal) and 10.85±0.29mm (at 4mm depth) . At the end of the healing period, at the same levels the width of the ridge decreased by 0.51±0.14mm and 0.63±0.17mm (p> 0.05), and was obtained 8.99±0.28mm and 10.22± 0.26mm. Conclusions: Following the analyzed data, the creation of bone supply through autogenous blocks (Khoury technique) allows to obtain predictable results and a suitable bone bed for the insertion of dental implants. If the alveolar ridge is atrophied (thin) only in the coronary portion (in 8 cases out of 10), and the apical portion is thick enough, the insertion of dental implants simultaneously with the grafting procedure does not affect the graft integration.

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“…Using xenogenous bone blocks from both equine and autogenic sources for onlay mandibular augmentation in rabbits, a recent study compared the incorporation and remodeling processes. Although the bone volume and quality of the grafted area were superior for autogenous bone, the amount of new bone formation after 60 days was similar for autologous and xenogeneic bone blocks [29]. More recently, the healing of collagenated equine bone blocks used as either inlay or onlay for mandibular bone augmentation in rabbits has been assessed [30].…”

Section: Introductionmentioning

confidence: 99%

Implants Placed with a Ring Technique Using Inlay and Onlay Block Xenografts in the Mandible of Rabbits

Kaneko,

Xavier,

Morinaga

et al. 2023

Materials

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Background: Xenogenous bone has been proposed as an alternative to overcome the disadvantages of autogenous grafting. The aim of the present study was to study bone dynamics at inlay and onlay xenografts used for bone augmentation applying a ring technique. Methods: The bone at the lateral surface of the mandibular angle of 12 adult male New Zealand White rabbits was exposed bilaterally. The cortical layer received multiple perforations on one side of the mandible, and a xenograft block of collagenated cancellous equine bone, 7 mm in diameter and 3 mm in width, was fixed on the prepared surface using an implant (onlay group). On the opposite side, a defect 7 mm in diameter and 3 mm in depth was prepared, and the xenograft block was adapted to the defect and fixed with an implant (inlay group). Results: After ten weeks of healing, in the onlay grafts, new bone was mainly formed on the trabeculae surface, reaching in some specimens the most coronal regions of the block. In the inlay grafts, new bone was found arranged on the trabecular surfaces but also occupying the spaces among the trabeculae. The entrance of the defect was often found close to the top of the block by newly formed bone. A higher percentage of new bone was found in the inlay (19.0 ± 9.3%) compared to the onlay (10.4 ± 7.4%) groups (p = 0.031). The mean gain in osseointegration at the implant in relation to the base of the original 3 mm deep defect was 0.95 ± 1.05% in the onlay group and 0.78 ± 0.71% in the inlay group (p = 0.603). Conclusion: The inlay grafts exhibited a higher new bone percentage than the onlay block grafts possibly due to the defect conformation that presented more sources for bone growth. The trabecular conformation and the composition of the grafts made possible the expression of the osteoconductive properties of the material used. This resulted, in several specimens, in the growth of bone on the graft trabeculae toward the most superior regions in both groups and in the closure of the coronal entrance of the defects in the inlay group. The clinical relevance of this experiment is that the ring technique applied as an inlay method could be suitable for bone augmentation.

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Histomorphometric, Immunohistochemical and Microtomographic Comparison between Autogenous and Xenogenous Bone Blocks for Mandibular Lateral Augmentation in Rabbits

Cited by 7 publications

References 28 publications

Histological Comparison of Collagenated Cancellous Equine Bone Blocks Used as Inlay or Onlay for Lateral Bone Augmentation in Rabbits

Histological Comparison of Collagenated Cancellous Equine Bone Blocks Used as Inlay or Onlay for Lateral Bone Augmentation in Rabbits

Guided bone regeneration through autogenous laminated bone blocks

Implants Placed with a Ring Technique Using Inlay and Onlay Block Xenografts in the Mandible of Rabbits

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