Histomorphometric study on testes in rams fed with linseed oil rich in omega-3 and -6 polyunsaturated fatty acids

Abstract

Many researchers have pointed out that the intake of omega-3 and -6 fatty acids is beneficial to animal health and reproductive capacity, but the effect of fatty acids on the histomorphology of ram testes is still unknown. The aim of this study was to investigate the effect of linseed oil containing polyunsaturated fatty acids on the morphology and histomorphometric characteristics of ram testis and epididymis. Testes of 12 Lithuanian local coarse wool rams were investigated histomorphologically and histomorphometrically in the present work. The rams were divided into two groups, an experimental group and a control group following, the principle of analogues – the same origin, age, weight, and condition score. The experimental group (n = 6) received 2% linseed oil sediments while the control group (n = 6) received 1.2% sunflower oil for 9 months. The diameter of testicular tubules and the height of the germinal epithelium, the spermatogenic index and the number of Leydig cells were higher in the experimental group than those in the control group. In addition, the number of degenerated tubules was not significantly lower in the group that received 2% linseed oil sediments. Still, the height of the epididymal epithelium was lower than that in the control group....

Ameliorating Effect of Linseed Oil Against Fluconazole Induced Adverse Effect on Male Fertility of Cocks

“…As showed in the obtained data, linseed oil supplemented group after fluconazole medication showed a slight increase in amount of spermatozoids in epididymal lumen with slightly decreased hyperplastic alteration in the lining epithelium, besides presence of few exfoliated germ cells and round bodies in the epididymal lumen. Similar Advances in Animal and Veterinary Sciences findings were obtained by Vaškas et al (2017) who said that epididymis has normal epithelium lining in linseed supplemented rams. The LH receptor (LHR) is critical for mammalian male sexual development and reproductive function.…”

“…Also, Feng et al (2015) detected that n-3 and n-6 PUFA have increased the spermatogonial development and germ cell layers in testes of supplemented rooster. Moreover, Abu-Heakal et al ( 2016) and Vaškas et al (2017) reported a histopathological improvement in the examined testis of 2% linseed oil treated male rabbits that showed a prominent increased in lumen diameter of seminiferous tubules and thickness of interstitial tissue. Epididymal sections from fluconazole treated group showed marked decrease in diameter of epididymal duct and decreased amount of spermatozoids in lumen with presence of large amount of exfoliated germ cells and round bodies in the lumen.…”