Histomorphometrical analysis of new bone obtained by distraction osteogenesis and osteogenesis by periosteal distraction in rabbits

Şençimen, Metin; Aydıntuğ, Yavuz Sinan; Ortakoğlu, Kerim; Karslioğlu, Yıldırım; Günhan, Ömer; Günaydin, Yılmaz

doi:10.1016/j.ijom.2006.08.016

Cited by 61 publications

(82 citation statements)

References 33 publications

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“…16,18,27 On the contrary, fat tissue formation has not been observed in rats even when the contribution of periosteum was deliberately severed. 28 A compartment-specific anabolic response has been noticed on the periosteal progenitors from frontal and parietal bone; osteoblastic and adipogenic differentiation in these populations was influenced by embryonic lineage and developmental origin.…”

Section: Discussionmentioning

confidence: 99%

Comparison of two protocols of periosteal distraction osteogenesis in a rabbit calvaria model

et al. 2015

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The regenerative pathways during periosteal distraction osteogenesis may be influenced by the local environment composed by cells, growth factors, nutrition and mechanical load. The aim of the present study was to evaluate the influence of two protocols of periosteal distraction on bone formation. Custom made distraction devices were surgically fixed onto the calvariae of 60 rabbits.After an initial healing period of 7 days, two groups of animals were submitted to distraction rates of 0.25 and 0.5 mm/24h for 10 days, respectively. Six animals per group were sacrificed 10 (middistraction), 17 (end-distraction), 24 (1-week consolidation), 31 (2-week consolidation) and 77 days (2-month consolidation) after surgery. Newly formed bone was assessed by means of micro-CT and histologically. Expression of transcripts encoding tissue-specific genes (BMP-2, RUNX2, ACP5, SPARC, collagen I α1, collagen II α1 and SOX9) was analyzed by quantitative PCR. Two patterns of bone formation were observed, originating from the old bone surface in Group I and from the periosteum in Group II. Bone volume and bone mineral density significantly increased up to the 2-month consolidation period within the groups (p <0.05). Significantly more bone was observed in Group II compared to Group I at the 2-month consolidation period (p <0.001). Expression of transcripts encoding osteogenic genes in bone depended on the time-point of observation (p <0.05).Low level of transcripts reveals an indirect role of periosteum in the osteogenic process. Two protocols of periosteal distraction in the present model resulted in moderate differences in terms of bone formation.

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Section: Discussionmentioning

confidence: 99%

Comparison of two protocols of periosteal distraction osteogenesis in a rabbit calvaria model

et al. 2015

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“…29 In most studies of DO for long bones or mandible lengthening, the authors have reported new bone formation by intramembranous ossification. 29,30 In the present study, it was seen that there was no cartilaginous or fibrocartilaginous tissue in newly formed bone, and the ossification was defined as intramembranous. When α-tocopherol was injected into the area activated by inter-premaxillary suture expansion during the early stage, bone formation in the suture was markedly stimulated in a dose-dependent manner consistent with the previous report testing TGF-β1.…”

Section: Discussionmentioning

confidence: 55%

Stimulation of bone formation in the expanding inter-premaxillary suture by vitamin E, in rat

et al. 2009

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Objective: The aim of this study was to evaluate the effects of vitamin E (α-tocopherol) administration on bone formation in response to expansion of the inter-premaxillary suture, in rats, histomorphometrically. Methods: Thirty 50 -60 day old Wistar rats were separated into five equal groups (one control and four experimental). All groups were subjected to inter-premaxilla expansion with 50-gram of force. Six control animals received saline solution (Group I) and three experimental groups were treated with a single dose of α-tocopherol injected into the inter-premaxillary suture after one day after appliance placement (Group II: 2 mg/kg; Group III: 10 mg/kg; and Group IV: 50 mg/kg). A further group of six animals received three injections of 10 mg/kg α-tocopherol, one each on days 3, 6, and 9 (Group V). Bone formation in the suture was evaluated by bone histomorphometry. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p ＜ 0.05 level. Results: New bone area, bone perimeter, feret's diameter and newly formed bone measurements were significantly higher in the experimental groups than the control (p ＜ 0.001). Bone architecture in α-tocopherol administrated groups was improved, and bone formation during the expansion period was stimulated significantly, in a dose-dependent manner. Conclusions: The application of α-tocopherol during the early stages to orthopedically expanded inter-premaxillary suture areas may stimulate bone formation and shorten the retention period, in rats.

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“…Fracture and dislocation of bone fragments and wound dehiscence have been reported 3) . In contrast, bone augmentation by periosteal distraction has been successful in the mandible and skull of rabbits 6,7) and in the forehead of pigs 8) .…”

Section: Discussionmentioning

confidence: 99%

Image Analysis of Lateral Alveolar Ridge Augmentation using Periosteal Distraction Osteogenesis

Inoue

Kuroiwa

Naitoh

et al. 2014

J. Hard Tissue Biology.

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The aim of this study was to evaluate a novel technique for inducing osteogenesis through original periosteal distraction in a canine mandible model. A periosteal distraction device was rigidly fixed to the mandible in three adult canines (i.e., six sides total). Two sides of 1 dog served as the control. In the experimental group, periosteal distraction was started 4 weeks after placement of the periosteal distraction device. The periosteum was distracted 0.5 mm per day until a height of 3 mm was reached. Distraction continued for 8 weeks. The animals were killed 14 weeks postoperatively. Radiographs were obtained after the devices were fitted and again after distracting the periosteum. Computed tomography (CT) was performed at weeks 1 and 8. Transverse images using OsiriX software were constructed based on the periosteal device to observe the amount of augmentation. Images over time were superimposed on the reference image using Photoshop software. It revealed the changes with time, which were measured. All 6 periosteal distraction devices remained rigidly fixed to the lateral surface of the mandibles. At completion of distraction, all operative sites had healed without evidence of infection or wound dehiscence. The amount of bone augmentation was calculated by comparing CT imagees. The experimental group showed increased width in the area of bone growth and in the bone itself on all six sides. Average increases were 0.7 mm in bone width and 3.31 mm in the area. Bone area and bone width were decreased slightly in the control group. There were significant differences between controls and the experimental group regarding bone width and bone area. We achieved bone augmentation using a custom-made periosteal distraction device. The amount of bone augmentation was calculated by comparing CT images.

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Histomorphometrical analysis of new bone obtained by distraction osteogenesis and osteogenesis by periosteal distraction in rabbits

Cited by 61 publications

References 33 publications

Comparison of two protocols of periosteal distraction osteogenesis in a rabbit calvaria model

Comparison of two protocols of periosteal distraction osteogenesis in a rabbit calvaria model

Stimulation of bone formation in the expanding inter-premaxillary suture by vitamin E, in rat

Image Analysis of Lateral Alveolar Ridge Augmentation using Periosteal Distraction Osteogenesis

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