Histone acetylation in the olfactory bulb of young rats facilitates aversive olfactory learning and synaptic plasticity

Wang, Y.-J.; Okutani, Fumino; Murata, Yuzo; Taniguchi, Masaru; Namba, Toshiharu; Kaba, Hideto

doi:10.1016/j.neuroscience.2012.12.015

Cited by 13 publications

(9 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells with immunoreactivity above this threshold were counted as Ac‐H3 immuno‐positive cells as classically carried out in other studies on epigenetics (Wang et al . ). Ac‐H3 immuno‐positive cells were counted in each of the brain regions recorded and then normalized in areas of 100 000 µm 2 .…”

Section: Methodsmentioning

confidence: 97%

The histone deacetylase inhibitor sodium butyrate decreases excessive ethanol intake in dependent animals

et al. 2014

View full text Add to dashboard Cite

Converging evidence indicates that epigenetic mechanisms are involved in drug addiction, and that enzymes involved in chromatin remodeling may represent interesting targets in addiction treatment. No study has addressed whether histone deacetylase (HDAC) inhibitors (HDACi) can reduce excessive ethanol intake or prevent relapse in alcohol-dependent animals. Here, we assessed the effects of two HDACi, sodium butyrate (NaB) and MS-275, in the operant ethanol self-administration paradigm in dependent and non-dependent rats. To characterize some of the epigenetic mechanisms associated with alcohol dependence and NaB treatment, we measured the levels of histone H3 acetylation in different brain areas of dependent and non-dependent rats, submitted or not to NaB treatment. Our results demonstrated that (1) NaB and MS-275 strongly decreased excessive alcohol intake of dependent rats in the operant ethanol self-administration paradigm but not of non-dependent rats; (2) NaB reduced excessive drinking and prevented the escalation of ethanol intake in the intermittent access to 20% ethanol paradigm; and (3) NaB completely blocked the increase of ethanol consumption induced by an alcohol deprivation, thus demonstrating a preventive effect of NaB on relapse. The mapping of cerebral histone H3 acetylation revealed a hyperacetylation in the amygdala and cortical areas in dependent rats. Interestingly, NaB did not exacerbate the hyperacetylation observed in these regions, but instead restored it, specifically in cortical areas. Altogether, our results clearly demonstrated the efficacy of NaB in preventing excessive ethanol intake and relapse and support the hypothesis that HDACi may have a potential use in alcohol addiction treatment.

show abstract

Section: Methodsmentioning

confidence: 97%

The histone deacetylase inhibitor sodium butyrate decreases excessive ethanol intake in dependent animals

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In our experiments, we established a model in which early odor preference memory was extended for at least 9 d after one trial training on PND 6 consisting of 10 min odor + stroking training combined with intrabulbar infusion of TSA prior to training. Wang et al (2013) had previously reported that odor + intrabulbar TSA at the same dose on PND11 produced odor aversion even without footshock. This suggested that intrabulbar TSA in older pups readily induced aversion.…”

Section: Summary Of Major Findingsmentioning

confidence: 94%

“…We also found that prolonged memory was specific to the paired odor consistent with a selective role of TSA in enhancing normal memory pathways. A pairing control was not used in the TSA aversive pup learning model (Wang et al 2013) and may be important to examine since aversive odor learning established using intrabulbar disinhibition as the unconditioned stimulus produces nonpairing specific odor aversion (Okutani et al 1999).…”

Section: Summary Of Major Findingsmentioning

confidence: 99%

“…TSA (Cedarlane) was dissolved in 100% dimethyl sulfoxide (DMSO, EMD Chemicals Inc.) to prepare a stock solution (stock concentration 1 µg/µL) and aliquoted as 10 µL in each vial to store at −20°C. To make the experimental concentration, 10 µL TSA was diluted with 190 µL of 10% DMSO to make 200 µL total volume (working concentration 0.05 µg/µL) as described previously (Wang et al 2013). One microliter of 0.05 µg tubacin (Sigma-Aldrich) was prepared in the same manner as TSA and in the same concentration as TSA.…”

Section: Drug Preparationmentioning

confidence: 99%

“…Their work suggested that CBP-CREB interactions and histone acetylation provide regulatory mechanisms for enhancing memory and synaptic plasticity (Korzus et al 2004;Vecsey et al 2007). Recent findings also show that intrabulbar TSA infusion facilitates the duration of aversive olfactory learning in young rats (Wang et al 2013). However, all of these TSA studies are related to aversive learning.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Histone deacetylase inhibition induces odor preference memory extension and maintains enhanced AMPA receptor expression in the rat pup model

et al. 2017

View full text Add to dashboard Cite

Histone deacetylase (HDAC) plays a role in synaptic plasticity and long-term memory formation. We hypothesized that trichostatin-A (TSA), an HDAC inhibitor, would promote long-term odor preference memory and maintain enhanced GluA1 receptor levels that have been hypothesized to support memory. We used an early odor preference learning model in neonate rat pups that normally produces only 24-h memory to test behavior and examine receptor protein expression. Our behavioral studies showed that intrabulbar infusion of TSA, prior to pairing of the conditioned stimulus (peppermint odor) with the unconditioned stimulus (tactile stimulation), prolonged 24-h odor preference memory for at least 9 d. The prolonged odor preference memory was selective for the paired odor and was also observed using a specific HDAC6 inhibitor, tubacin, supporting a role for histone acetylation in associative memory. Immunoblot analysis showed that GluA1 receptor membrane expression in the olfactory bulbs of the TSA-treated group was significantly increased at 48 h unlike control rats without TSA. Immunohistochemistry revealed significant increase of GluA1 expression in olfactory bulb glomeruli 5 d after training. These results extend previous evidence for a close relationship between enhanced GluA1 receptor membrane expression and memory expression. Together, these findings provide a new single-trial appetitive model for understanding the support and maintenance of memories of varying duration.

show abstract

Signaling mechanisms underlying activity‐dependent integration of adult‐born neurons in the mouse olfactory bulb

Bao,

Romero,

Belfort

et al. 2024

Genesis

View full text Add to dashboard Cite

SummaryAdult neurogenesis has fascinated the field of neuroscience for decades given the prospects of harnessing mechanisms that facilitate the rewiring and/or replacement of adult brain tissue. The subgranular zone of the hippocampus and the subventricular zone of the lateral ventricle are the two main areas in the brain that exhibit ongoing neurogenesis. Of these, adult‐born neurons within the olfactory bulb have proven to be a powerful model for studying circuit plasticity, providing a broad and accessible avenue into neuron development, migration, and continued circuit integration within adult brain tissue. This review focuses on some of the recognized molecular and signaling mechanisms underlying activity‐dependent adult‐born neuron development. Notably, olfactory activity and behavioral states contribute to adult‐born neuron plasticity through sensory and centrifugal inputs, in which calcium‐dependent transcriptional programs, local translation, and neuropeptide signaling play important roles. This review also highlights areas of needed continued investigation to better understand the remarkable phenomenon of adult‐born neuron integration.

show abstract

Histone acetylation in the olfactory bulb of young rats facilitates aversive olfactory learning and synaptic plasticity

Cited by 13 publications

References 47 publications

The histone deacetylase inhibitor sodium butyrate decreases excessive ethanol intake in dependent animals

The histone deacetylase inhibitor sodium butyrate decreases excessive ethanol intake in dependent animals

Histone deacetylase inhibition induces odor preference memory extension and maintains enhanced AMPA receptor expression in the rat pup model

Signaling mechanisms underlying activity‐dependent integration of adult‐born neurons in the mouse olfactory bulb

Contact Info

Product

Resources

About