Histone deacetylase HDA19 interacts with histone methyltransferase SUVH5 to regulate seed dormancy in Arabidopsis

Abstract

Seed dormancy controls the timing of germination and plays a significant role in adaptation and evolution of seed plants. In this study, a yeast two‐hybrid, pull‐down assay and co‐immunoprecipitation assay were used to ascertain the protein relationship of SUVH5 and HDA19. Both qRT‐PCR and ChIP‐qPCR were used to examine the molecular mechanism of how HDA19 and SUVH5 regulate seed dormancy. The results demonstrated that histone methyltransferase SUVH5 interacted with histone deacetylase HDA19 in vivo and in vitro. In addition, they showed that mutants of HDA19 could deepen seed dormancy, and that SUVH5 had the same effect. The hda19 suvh5 double mutant displayed a higher level of seed dormancy than the single mutants hda19 or suvh5. Moreover, the expression of seed dormancy‐related genes increased in hda19, suvh5 and in hda19 suvh5 double mutant plants, which was associated with increased histone H3 acetylation (H3ac), but decreased histone H3 Lys 9 dimethylation (H3K9me2). ChIP assays proved that HDA19 could directly integrate into the chromatin of genes regulating seed dormancy. Taken together, our results show that HDA19 and SUVH5 work together and have a negative role in seed dormancy.

Genomic imprinted genes in reciprocal hybrid endosperm of Brassica napus

“…BnaA05g08770D, an overlapped imprinted gene with B. rapa, is a homolog of AtSUVH5. AtSUVH5 interacts with AtHDA19 and negatively regulates seed dormancy [70].…”

Poplar acetylome profiling reveals lysine acetylation dynamics in seasonal bud dormancy release

“…Acetylation is generated by KAT-catalysed transfer of an acetyl group from acetyl-CoA to the ε-amino side chain of lysine and is reversed by KDACs (Verdin & Ott, 2015). Kac, especially histone Kac, has been extensively studied and found to exert important functions in various biological processes, including seed germination, organ development, flowering, biotic and abiotic stress response and leaf senescence (Colville et al, 2011;David Law & Suttle, 2004;Hollender & Liu, 2008;Hu, Lu, Zhao, & Zhou, 2019;Kim, Latrasse, Servet, & Zhou, 2013;Park et al, 2019;van Zanten et al, 2014;Yuan, Chen, Chen, Wu, & Huang, 2019;Zheng et al, 2016;Zhou, Yang, Zhang, Luo, & Xie, 2020). Over the past decade, advances in mass spectrometrybased proteomics have vastly provided an unbiased view of the lysine acetylome and revealed new insights into the scope and regulation of non-histone Kac (Choudhary et al, 2014;Narita et al, 2019).…”

Genome-wide analysis of histone acetyltransferase and histone deacetylase families and their expression in fruit development and ripening stage of pepper (Capsicum annuum)

“…It can be learned from Arabidopsis that a light-regulated histone deacetylase HDA15 interacts with PIF1to suppress the light-responsive genes during seed germination in the dark ( Gu et al, 2017 ) and HDA15 also restricts chlorophyll biosynthesis- and photosynthesis-related genes through its interaction with PIF3 ( Liu et al, 2013 ). Likewise, HDA19 interacts with histone methyltransferase SUVH5 and regulates seed dormancy through ABA and GA signaling pathways by modulating overall histone H3 acetylation and H3K9me2 methylation on the promoter of the target genes ABI3 , RGA , and DOG1 ( Zhou et al, 2020 ; Kumar et al, 2021 ). The Arabidopsis MYST histone acetyltransferase HAM1/2 enhances the expression of the negative regulator FLC gene through H4K5 acetylation and results in delayed flowering ( Xiao et al, 2013 ).…”