Histone Deacetylase Inhibitor Improves the Development and Acetylation Levels of Cat–Cow Interspecies Cloned Embryos

Wittayarat, Manita; Sato, Yoko; Kim, Lanh Thi; Morita, Yasuhiro; Chatdarong, Kaywalee; Techakumphu, Mongkol; Taniguchi, Masayasu; Otoi, Takeshige

doi:10.1089/cell.2012.0094

Cited by 12 publications

(7 citation statements)

References 33 publications

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“…The present study showed that the high levels of acetylation on H3K9/18/23 were observed in the TSA‐treated iSCNT cat‐cow embryos either at the PN or two‐cell stage, which resembles the bovine IVF embryos in this study and is in agreement with previous studies in mouse IVF (H3K9), sheep IVF (H3K9), cat iSCNT/IVF (H3K9) and miniature pig SCNT/IVF (H3K18) (Hou et al ; Yamanaka et al ; Gómez et al ). The hyper‐acetylation at several lysine residues of histone (H3K9/18/23) by TSA within a limited time (PN to two‐cell stage) in the present study might promote the development to the blastocyst from an iSCNT cat‐cow embryo as we reported previously (Wittayarat et al ), because TSA would re‐establish the embryonic epigenetic characteristics and gene expression (Shi et al ; Wu et al ) for an easier entry of some essential transcriptional factors into the nucleosome to activate gene expression (Yamanaka et al ), stimulating more effective remodelling of the somatic chromatin (Rybouchkin et al ).…”

Section: Discussionsupporting

confidence: 69%

“…Immunofluorescence analysis was processed according to the protocol described in our previous study (Wittayarat et al ). The embryos at 2 h PF/PI, 6 h PF/PI, PN, two‐cell, four‐cell and eight‐cell stages from the 50 nmol/L TSA‐treated iSCNT, TSA‐untreated control iSCNT and IVF groups were subjected to H3K9/18/23 ac immunofluorescence analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The mean intensity in each examined nucleus was recorded. The relative intensity levels of H3K9/18/23 ac and H3K9me2 in each nucleus were calculated using the following formula the same as in Wittayarat et al ().

Relative intensity normalin one nucleus = \frac{\begin{array}{c} center & normalH 3 normalK 9 true/ 18 true/ 23 normala normalc or normalH 3 normalK 9 me 2 true(Mean intensity of nucleus \\ center & ‐ mean intensity of cytoplasm true) \end{array}}{DAPI ((), Mean intensity of nucleus ‐ mean intensity of cytoplasm)}

…”

Section: Methodsmentioning

confidence: 99%

“…The histone deacetylase (HDAC) inhibitors such as trichostatin A (TSA) can cause the accumulation of highly acetylated histone species within cells, resulting in a variety of phenotypic changes (Nakajima et al ). Previously we showed that increasing H3K9ac levels in iSCNT cat‐cow embryos by 50 nmo/L TSA treatment for 24 h after fusion could support embryo development until the blastocyst stage (Wittayarat et al ). However, other covalent histone modifications at preimplantation embryo stages are still not defined.…”

Section: Introductionmentioning

confidence: 93%

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Epigenetic modulation on cat‐cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A

Wittayarat

Sato

Kim

et al. 2016

Animal Science Journal

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This study aimed to determine the acetylation at lysine 9/18/23 of histone H3 (H3K9ac/H3K18ac/H3K23ac; H3K9/18/23 ac) and the di-methylation at lysine 9 of histone H3 (H3K9me2) during early embryogenesis among trichostatin A (TSA)-treated interspecies somatic cell nuclear transfer (iSCNT) cat-cow (TSA-iSCNT) embryos, TSA-untreated iSCNT cat-cow control (control) embryos and bovine in vitro fertilization (IVF) embryos, because TSA-iSCNT embryos can develop to blastocysts. Compared to the control embryos, higher expressions of H3K9/18/23 ac were observed in TSA-iSCNT embryos and IVF embryos at most following stages (2 h post-fusion / post-insemination (PF/PI) to eight-cell stage). At 6 h PF/PI the expression of H3K9/23 ac in TSA-iSCNT embryos and IVF embryos were lower than those in control embryos, and the expression of H3K18ac was no difference among the three groups. The expression of H3K9/23 ac increased in TSA-iSCNT embryos and IVF embryos at pronuclear (PN) stages. The expression of H3K9me2 in TSA-iSCNT embryos resembled that of IVF embryos at 2 h PF/PI to PN stages, and these expression levels were greater than those of control embryos. These results suggest that treatment of iSCNT embryos with TSA modifies the patterns of histone modification at certain lysine residues in a manner that is comparable with that seen in IVF during early embryogenesis.

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Section: Discussionsupporting

confidence: 69%

Section: Methodsmentioning

confidence: 99%

Relative intensity normalin one nucleus = \frac{\begin{array}{c} center & normalH 3 normalK 9 true/ 18 true/ 23 normala normalc or normalH 3 normalK 9 me 2 true(Mean intensity of nucleus \\ center & ‐ mean intensity of cytoplasm true) \end{array}}{DAPI ((), Mean intensity of nucleus ‐ mean intensity of cytoplasm)}

…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

See 2 more Smart Citations

Epigenetic modulation on cat‐cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A

Wittayarat

Sato

Kim

et al. 2016

Animal Science Journal

Self Cite

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“…Moreover, a previous study has shown that acH3K9 in SCNT embryos was increased with TSA treatment and reached similar levels to that of the IVF embryos [15]. As for acH4K5, it is found at low levels in bovine cloned embryos and can also be enhanced by TSA treatment [16].…”

Section: Discussionmentioning

confidence: 71%

Valproic Acid Improves Porcine Parthenogenetic Embryo Development Through Transient Remodeling of Histone Modifiers

Huang

Yuan

et al. 2015

Cell Physiol Biochem

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Background/Aims: Parthenogenetic embryos are useful in many applications, such as being an alternative source of embryonic stem cells that would avoid ethical problems. Aberrance in epigenetic reprogramming is considered the major reason for the developmental failure of parthenogenetic embryos. Many histone deacetylase inhibitors have been shown to improve the reprogramming of stem cells and embryos. Here, the relationship between histone modification and parthenogenetic embryonic development was explored. Methods: Valproic acid (VPA) treatment was applied during the culture of parthenogenetic embryos. The abundance of histone modifiers was examined by immunofluorescence and quantified by Image-pro software. Results: The acH3K9 level in in vitro fertilized embryos was significantly higher than parthenogenetic embryos. VPA treatment improved both the blastocyst formation rate and the acH3K9 level in parthenogenetic embryos. The signal intensities of acH4K5 and H3K4me2 were also enhanced in VPA treated embryos. The H3K27me2 level was decreased in the VPA treated embryos at the 2-cell stage. However, the enhancement in the acH3K9, acH4K5 and H3K4me2 level, or the decrease in the H3K27me2 level disappeared shortly after VPA withdrawal. Conclusion: Optimizing histone modifications for a short time following activation was sufficient to enhance the in vitro development of parthenogenetic embryos.

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Interspecies nuclear transfer using fibroblasts from leopard, tiger, and lion ear piece collected postmortem as donor cells and rabbit oocytes as recipients

Mahesh

Suman

Katari

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

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Skin fibroblast cells were obtained from a small piece of an ear of leopard, lion, and tiger collected postmortem and attempts were made to synchronize the skin fibroblasts at G0/G1 of cell cycle using three different approaches. Efficiency of the approaches was tested following interspecies nuclear transfer with rabbit oocytes as recipient cytoplasm. Fluorescence-activated cell sorting revealed that the proportion of G0/G1 cells increased significantly (P < 0.05) when cells subjected to serum starvation, contact inhibition, and 3 mM sodium butyrate (NaBu) treatment when compared with cycling cells. However, 3 mM NaBu treatment caused alterations in cell morphology and increase in dead cells. Thus, interspecies nuclear transfer was carried out using fibroblast cells subjected to contact inhibition for 72 h, serum starvation for 48 h, and cells treated with 1.0 mM NaBu for 48 h. The fusion rates, the proportion of fused couplets that cleaved to two-cell and developed to blastocyst, were highest in all three species when the donor cells were treated with 1.0 mM NaBu for 48 h. But, the blastocyst percentage of interspecies nuclear embryos (5-6%) was significantly lower when compared with rabbit-rabbit nuclear transfer embryos (22.9%). In conclusion, fibroblast cells of leopard, lion, and tiger were successfully synchronized and used for the development of blastocysts using rabbit oocytes as recipient cytoplasm.

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Histone Deacetylase Inhibitor Improves the Development and Acetylation Levels of Cat–Cow Interspecies Cloned Embryos

Cited by 12 publications

References 33 publications

Epigenetic modulation on cat‐cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A

Epigenetic modulation on cat‐cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A

Valproic Acid Improves Porcine Parthenogenetic Embryo Development Through Transient Remodeling of Histone Modifiers

Interspecies nuclear transfer using fibroblasts from leopard, tiger, and lion ear piece collected postmortem as donor cells and rabbit oocytes as recipients

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