Histone Deacetylase, Xanthine Oxidase and Urease Inhibitory Activities of Eremurus spectabilis M. Bieb. Extracts

Abstract

Objective: Natural ingredients derived from plants (especially edible ones) continue to be investigated for understanding whether they have therapeutic/preventive effects on certain ailments, such as cancer and gastrointestinal disorders. The main goal of the current work is to investigate the inhibitory potential of Eremurus spectabilis M. Bieb. extracts on histone deacetylase (HDAC), xanthine oxidase (XO) and urease activities.Material and Method: Fresh E. spectabilis leaves were obtained from a local market in Eyup/Istanbul. Inhibitory activities of ethanolic and ethyl acetate extracts on HDAC, XO and urease were examined.Results: According to our findings, ethanolic and ethyl acetate extracts of E. spectabili both have revealed apparent inhibitory effects against all examined enzymes. Ethanolic extract showed a better inhibitory effect on HDAC than ethyl acetate extract. On the other hand, the inhibitory effects of ethyl acetate extract against both XO and urease were higher than the ethanolic extract. Conclusion:Consequently, due to the inhibitory effects of the plant being studied, it might be suggested that it can be used for medicinal purposes, in raw extract form, or as a source of bioactive compounds.

Bazı etanolik bitki ekstrelerinin histon deasetilaz inhibitör ve antioksidan aktiviteleri: Kanser için yeni bir terapötik yaklaşım

“…For the 70% ethanol extracts preparation, 20 grams of plant material were extracted by Soxhlet apparatus with 200 mL 70% ethanol for 8 h until fully colorless. Ethanol was removed by rotary evaporation (Bibby, USA) under reduced pressure at 50°C 17 . Extracts were concentrated, dried and kept in the dark at -20°C for further experiments.…”

Antioxidant Activities of Eremurus spectabilis M. Bieb. Extracts and Sulfur Compounds

“…In a recent study, it was shown that ethanolic and ethyl acetate extracts of E. spectabilis M. Bieb. have an inhibitive action against histone deacetylase, xanthine oxidase, and urease enzymes (9). In addition, the aqueous extract of this plant exhibits inhibitory effects on diabetes mellitus and skinassociated enzymes (i.e., α-amylase, α-glucosidase, elastase, hyaluronidase, and tyrosinase, respectively) (10).…”