Histone H2A mobility is regulated by its tails and acetylation of core histone tails

Higashi, Taishi; Matsunaga, Sachihiro; Isobe, Keisuke; Morimoto, Akira; Shimada, Toshio; Kataoka, Shogo; Watanabe, Wataru; Uchiyama, Susumu; Itoh, Kazuyoshi; Fukui, Kiichi

doi:10.1016/j.bbrc.2007.03.203

Cited by 31 publications

(37 citation statements)

References 39 publications

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“…In agreement with our global analysis (supplementary material Fig. S1C), and consistent with previous biochemical and FRAP (fluorescence recovery after photobleaching) analyses in human cells (Bönisch et al, 2012;Higashi et al, 2007;Jackson and Chalkley, 1985;Kimura et al, 2006), within the detection range of our method both variants showed a comparable extensive deposition of newly synthesized histones in euchromatin. However, the situation proved to be different at the PHC domains, where the distribution of newly synthesized histones relative to euchromatin could be categorized into one of three distinct patterns -exclusion, even distribution or enrichment (Fig.…”

Section: Different Dynamics Of H2a and H2az Deposition At Phcsupporting

confidence: 93%

“…In contrast to CENP-A, the deposition dynamics of H2A variants at centromeres remain unknown. Notably, H2A variants constantly exchange at a rapid rate throughout the nucleus (Bönisch et al, 2012;Higashi et al, 2007;Jackson and Chalkley, 1985;Kimura et al, 2006), raising an important question regarding their stable maintenance at a given locus. Furthermore, given that H2A.Z displays a broader localization across the genome than CENP-A, its dynamics might be distinct in different genomic compartments.…”

Section: Introductionmentioning

confidence: 99%

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Pericentric heterochromatin state during the cell cycle controls the histone variant composition of centromeres

Boyarchuk¹,

Filipescu²,

Vassias³

et al. 2014

Journal of Cell Science

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BSTRACTCorrect chromosome segregation requires a unique chromatin environment at centromeres and in their vicinity. Here, we address how the deposition of canonical H2A and H2A.Z histone variants is controlled at pericentric heterochromatin (PHC). Whereas in euchromatin newly synthesized H2A and H2A.Z are deposited throughout the cell cycle, we reveal two discrete waves of deposition at PHC -during mid to late S phase in a replicationdependent manner for H2A and during G1 phase for H2A.Z. This G1 cell cycle restriction is lost when heterochromatin features are altered, leading to the accumulation of H2A.Z at the domain. Interestingly, compromising PHC integrity also impacts upon neighboring centric chromatin, increasing the amount of centromeric CENP-A without changing the timing of its deposition. We conclude that the higher-order chromatin structure at the pericentric domain influences dynamics at the nucleosomal level within centromeric chromatin. The two different modes of rearrangement of the PHC during the cell cycle provide distinct opportunities to replenish one or the other H2A variant, highlighting PHC integrity as a potential signal to regulate the deposition timing and stoichiometry of histone variants at the centromere.

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Section: Different Dynamics Of H2a and H2az Deposition At Phcsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Pericentric heterochromatin state during the cell cycle controls the histone variant composition of centromeres

Boyarchuk¹,

Filipescu²,

Vassias³

et al. 2014

Journal of Cell Science

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“…Histone H2A and its variants are actively exchanged (Higashi et al, 2007), and the equilibrium between their removal and incorporation determines their abundance in chromatin. The decrease in H2A, H2A.Z and macroH2A deposition in the nucleus after fertilization might have been caused by a decrease in the incorporation rate of these proteins into chromatin; H2A.Z and macroH2A were almost completely lost from the nucleus owing to their weak incorporation, and a low level of H2A was present in the nucleus because it was still incorporated in chromatin at a low level.…”

Section: Discussionmentioning

confidence: 99%

“…Because the Flag antibody was used to detect all these proteins, it was certain that the amount of incorporated H2A.X was much higher than those of other proteins. The replacement of H2A on chromatin is very fast: ~10% of the protein is replaced in 20 minutes (Higashi et al, 2007). Therefore, the levels of incorporation would reflect those of deposition in the nucleus, suggesting that H2A.X is abundant in the nuclei of early pre-implantation embryos.…”

Section: Developmentmentioning

confidence: 99%

Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice

et al. 2010

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SUMMARYHistone H2A has several variants, and changes in chromatin composition associated with their replacement might involve chromatin structure remodeling. We examined the dynamics of the canonical histone H2A and its three variants, H2A.X, H2A.Z and macroH2A, in the mouse during oogenesis and pre-implantation development when genome remodeling occurs. Immunocytochemistry with specific antibodies revealed that, although H2A and all variants were deposited in the nuclei of fullgrown oocytes, only histone H2A.X was abundant in the pronuclei of one-cell embryos after fertilization, in contrast with the low abundance of histone H2A and the absence of H2A.Z. The decline in H2A and the depletion of H2A.Z and macroH2A after fertilization were confirmed using Flag epitope-tagged H2A, H2A.Z and macroH2A transgenic mouse lines. Microinjection experiments with mRNA encoding the Flag-tagged proteins revealed a similar pattern of nuclear incorporation of the H2A variants. Fusion protein experiments using H2A, H2A.Z and macroH2A fused with the C-terminal 23 amino acids of H2A.X showed that the C-terminal amino acids of H2A.X function specifically to target this variant histone into chromatin in embryos after fertilization and that the absence of H2A.Z and macroH2A from the chromatin is required for normal development. These results suggest that global changes in the composition of histone H2A variants in chromatin play a role in genome remodeling after fertilization.

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“…To analyze chromatin dynamics, live imaging methods were developed for visualization of chromatins (Bystricky 2015). Various chromatin proteins including histones, condensins and a proliferating cell nuclear antigen can be fused with fluorescent proteins for chromatin visualization (Kanda et al 1998, Kimura and Cook 2001, Boisnard-Lorig et al 2001, Gerlich et al 2003, Fang and Spector 2005, Fujimoto et al 2005, Lermontova et al 2006, Higashi et al 2007, Takata et al 2007, Matsunaga and Umeda 2011, Nozaki et al 2014, Yokoyama et al 2016. However, these protein-based chromatin visualization methods label whole or partial chromatins and are not available for the detection of specific genomic loci.…”

mentioning

confidence: 99%

Chromatin Live Imaging with Genome Editing Techniques: Switching from Scissors to a Lamp

Fujimoto

Matsunaga

2016

CYTOLOGIA

Self Cite

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Summary Chromatin live imaging integrated with programmable DNA binding proteins derived from genome editing methods opens up the era of spatio-temporal analyses of chromatin dynamics. The DNA binding proteins, including a transcription activator-like effector (TALE) or a nuclease-dead CRISPR-associated protein 9 (dCas9), can be freely designed to bind a specific DNA sequence. Instead of a nuclease for DNA cleavage, a fluorescent protein is fused to TALE or dCAS9 for visualization of chromatin. They enable us to observe the endogenous genomic loci without the fixation of cells, the denaturation of DNA for hybridization and the insertion of exogenous DNA sequences into targeted loci. Using these live imaging systems dependent on the interaction between cis endogenous DNA sequence and trans DNA binding proteins, we can analyze chromatin dynamics in living cells. Multicolor chromatin imaging based on different dCas9 proteins will be a powerful cytogenetical technique instead of multicolor FISH and chromosome painting.

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Histone H2A mobility is regulated by its tails and acetylation of core histone tails

Cited by 31 publications

References 39 publications

Pericentric heterochromatin state during the cell cycle controls the histone variant composition of centromeres

Pericentric heterochromatin state during the cell cycle controls the histone variant composition of centromeres

Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice

Chromatin Live Imaging with Genome Editing Techniques: Switching from Scissors to a Lamp

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